Hepatic ultrastructural changes induced by the toxin microcystin-LR (MCLR) in mice

S. J. Hermansky, Rodney Smith Markin, E. H. Fowler, S. J. Stohs

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Microcystin-LR (MCLR) is a cyclic heptapeptide produced by the blue-green algae Microcystis aeruginosa. It is highly toxic and causes death in rodents due to hypovolemic shock with associated intrahepatic hemorrhage. The molecular mechanism of toxicity is unknown. In order to provide additional information regarding the toxicity of MCLR, the ultrastructural changes present in livers of mice following the administration of 100 μg MCLR/kg intraperitoneally (i.p.) were examined. Time-dependent changes were observed. Disruption of cell to cell contact occurred with infiltration of erythrocytes 60 min after MCLR treatment. Hepatocyte distortion, mitochondrial aggregation, and the prominent accumulation of large areas of endoplasmic reticulum were observed. No detectable hepatic changes in sinusoidal endothelial cells were present. The results suggest that MCLR preferentially affects hepatocytes, although the observations do not preclude the involvement of hepatic vasculature in the toxicity of MCLR.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalJournal of Environmental Pathology, Toxicology and Oncology
Volume12
Issue number2
StatePublished - Jan 1 1993

Fingerprint

toxin
toxicity
Liver
Toxicity
rodent
cyanobacterium
Hepatocytes
infiltration
Microcystis
Poisons
Endothelial cells
Cyanobacteria
Infiltration
Endoplasmic Reticulum
toxin-LR
cyanoginosin LR
Cause of Death
Rodentia
Shock
Agglomeration

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Hepatic ultrastructural changes induced by the toxin microcystin-LR (MCLR) in mice. / Hermansky, S. J.; Markin, Rodney Smith; Fowler, E. H.; Stohs, S. J.

In: Journal of Environmental Pathology, Toxicology and Oncology, Vol. 12, No. 2, 01.01.1993, p. 101-106.

Research output: Contribution to journalArticle

@article{8ce49657146d4062b92c2160d436bb36,
title = "Hepatic ultrastructural changes induced by the toxin microcystin-LR (MCLR) in mice",
abstract = "Microcystin-LR (MCLR) is a cyclic heptapeptide produced by the blue-green algae Microcystis aeruginosa. It is highly toxic and causes death in rodents due to hypovolemic shock with associated intrahepatic hemorrhage. The molecular mechanism of toxicity is unknown. In order to provide additional information regarding the toxicity of MCLR, the ultrastructural changes present in livers of mice following the administration of 100 μg MCLR/kg intraperitoneally (i.p.) were examined. Time-dependent changes were observed. Disruption of cell to cell contact occurred with infiltration of erythrocytes 60 min after MCLR treatment. Hepatocyte distortion, mitochondrial aggregation, and the prominent accumulation of large areas of endoplasmic reticulum were observed. No detectable hepatic changes in sinusoidal endothelial cells were present. The results suggest that MCLR preferentially affects hepatocytes, although the observations do not preclude the involvement of hepatic vasculature in the toxicity of MCLR.",
author = "Hermansky, {S. J.} and Markin, {Rodney Smith} and Fowler, {E. H.} and Stohs, {S. J.}",
year = "1993",
month = "1",
day = "1",
language = "English (US)",
volume = "12",
pages = "101--106",
journal = "Journal of Environmental Pathology, Toxicology and Oncology",
issn = "0731-8898",
publisher = "Begell House Inc.",
number = "2",

}

TY - JOUR

T1 - Hepatic ultrastructural changes induced by the toxin microcystin-LR (MCLR) in mice

AU - Hermansky, S. J.

AU - Markin, Rodney Smith

AU - Fowler, E. H.

AU - Stohs, S. J.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Microcystin-LR (MCLR) is a cyclic heptapeptide produced by the blue-green algae Microcystis aeruginosa. It is highly toxic and causes death in rodents due to hypovolemic shock with associated intrahepatic hemorrhage. The molecular mechanism of toxicity is unknown. In order to provide additional information regarding the toxicity of MCLR, the ultrastructural changes present in livers of mice following the administration of 100 μg MCLR/kg intraperitoneally (i.p.) were examined. Time-dependent changes were observed. Disruption of cell to cell contact occurred with infiltration of erythrocytes 60 min after MCLR treatment. Hepatocyte distortion, mitochondrial aggregation, and the prominent accumulation of large areas of endoplasmic reticulum were observed. No detectable hepatic changes in sinusoidal endothelial cells were present. The results suggest that MCLR preferentially affects hepatocytes, although the observations do not preclude the involvement of hepatic vasculature in the toxicity of MCLR.

AB - Microcystin-LR (MCLR) is a cyclic heptapeptide produced by the blue-green algae Microcystis aeruginosa. It is highly toxic and causes death in rodents due to hypovolemic shock with associated intrahepatic hemorrhage. The molecular mechanism of toxicity is unknown. In order to provide additional information regarding the toxicity of MCLR, the ultrastructural changes present in livers of mice following the administration of 100 μg MCLR/kg intraperitoneally (i.p.) were examined. Time-dependent changes were observed. Disruption of cell to cell contact occurred with infiltration of erythrocytes 60 min after MCLR treatment. Hepatocyte distortion, mitochondrial aggregation, and the prominent accumulation of large areas of endoplasmic reticulum were observed. No detectable hepatic changes in sinusoidal endothelial cells were present. The results suggest that MCLR preferentially affects hepatocytes, although the observations do not preclude the involvement of hepatic vasculature in the toxicity of MCLR.

UR - http://www.scopus.com/inward/record.url?scp=0027218245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027218245&partnerID=8YFLogxK

M3 - Article

VL - 12

SP - 101

EP - 106

JO - Journal of Environmental Pathology, Toxicology and Oncology

JF - Journal of Environmental Pathology, Toxicology and Oncology

SN - 0731-8898

IS - 2

ER -