Hepatic protein expression networks associated with masculinization in the female fathead minnow (Pimephales promelas)

Christopher J. Martyniuk, Sophie Alvarez, Bonnie P. Lo, James R. Elphick, Vicki L. Marlatt

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Endocrine disruptors that act via the androgen receptor (AR) are less well studied than environmental estrogens, and there is evidence that treatment with AR agonists can result in masculinization of female fish. In this study, female fathead minnows (FHM) were exposed to the model nonaromatizable androgen 5-alpha dihydrotestosterone (DHT) (100 μg/L), the ureic-based herbicide linuron (LIN) (100 μg/L), and a mixture of DHT and LIN (100 μg/L each) to better characterize androgen action in females. LIN was used because of reports that this chemical has an antiandrogenic mode of action in fish. After 21d, DHT and LIN treatments resulted in a significant depression of plasma vitellogenin (Vtg) and DHT and DHT + LIN increased the prevalence of nuptial tubercles in female FHMs indicating masculinization. Using iTRAQ and an LTQ Orbitrap Velos, ∼2000 proteins were identified in the FHM liver and the number of proteins quantified after exposures was >1200. Proteins that significantly and consistently changed in abundance across biological replicates included prostaglandin E synthase 3, programmed cell death 4a, glutathione S transferases, canopy, selenoprotein U, and ribosomal proteins. Subnetwork enrichment analysis identified that interferon and epidermal growth factor signaling were regulated by DHT and LIN, suggesting that these signaling pathways are correlated to depressed plasma vitellogenin. These data provide novel insight into hepatic protein networks that are associated with the process of masculinization in teleosts.

Original languageEnglish (US)
Pages (from-to)4147-4161
Number of pages15
JournalJournal of proteome research
Volume11
Issue number8
DOIs
StatePublished - Aug 3 2012

Fingerprint

Linuron
Cyprinidae
Dihydrotestosterone
Liver
Androgens
Vitellogenins
Proteins
Fish
Fishes
Selenoproteins
Plasmas
Endocrine Disruptors
Ribosomal Proteins
Androgen Receptors
Herbicides
Cell death
Prostaglandins E
Glutathione Transferase
Epidermal Growth Factor
Interferons

Keywords

  • androgens
  • ecotoxicoproteomics
  • iTRAQ
  • subnetwork enrichment analysis
  • vitellogenin

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Hepatic protein expression networks associated with masculinization in the female fathead minnow (Pimephales promelas). / Martyniuk, Christopher J.; Alvarez, Sophie; Lo, Bonnie P.; Elphick, James R.; Marlatt, Vicki L.

In: Journal of proteome research, Vol. 11, No. 8, 03.08.2012, p. 4147-4161.

Research output: Contribution to journalArticle

Martyniuk, Christopher J. ; Alvarez, Sophie ; Lo, Bonnie P. ; Elphick, James R. ; Marlatt, Vicki L. / Hepatic protein expression networks associated with masculinization in the female fathead minnow (Pimephales promelas). In: Journal of proteome research. 2012 ; Vol. 11, No. 8. pp. 4147-4161.
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