Hepatic irradiation augments engraftment of donor cells following hepatocyte transplantation

Kosho Yamanouchi, Hongchao Zhou, Namita Roy-Chowdhury, Frank Macaluso, Liping Liu, Toshiyuki Yamamoto, Govardhana Rao Yannam, Charles Arthur Enke, Timothy D. Solberg, Anthony B. Adelson, Jeffrey L. Platt, Ira J. Fox, Jayanta Roy-Chowdhury, Chandan Guha

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Abstract

Engraftment of donor hepatocytes is a critical step that determines the success of hepatocyte transplantation. Rapid and efficient integration of donor cells would enable prompt liver repopulation of these cells in response to selective proliferative stimuli offered by a preparative regimen. We have earlier demonstrated that hepatic irradiation (HIR) in combination with a variety of hepatotrophic growth signals, such as partial hepatectomy and hepatocyte growth factor, can be used as a preparative regimen for liver repopulation of transplanted hepatocytes. In this study, we investigated the effects of HIR on engraftment of transplanted dipeptidyl peptidase IV (DPPIV)-positive hepatocytes in congeneic DPPIV-deficient rats. HIR-induced apoptosis of hepatic sinusoidal endothelial cells (SEC) within 6 hours of HIR resulted in dehiscence of the SEC lining in 24 hours. Although there was no change of the number of Kupffer cells after HIR, colloidal carbon clearance decreased 24 hours post HIR, indicating a suppression of phagocytic function. DPPIV + donor cells were transplanted 24 hours after HIR (0-50 Gy). There was an HIR dose-dependent increase in the donor hepatocyte mass engrafted in the liver parenchyma. The number of viable transplanted hepatocytes present in hepatic sinusoids or integrated in the parenchyma was greater in the HIR-treated group at 3 and 7 days after transplantation compared with the sham controls. Finally, we validated these rodent studies in cynomolgus monkeys, demonstrating that a single 10-Gy dose of HIR was sufficient to enhance engraftment of donor porcine hepatocytes. These data indicate that transient disruption of the SEC barrier and inhibition of the phagocytic function of Kupffer cells by HIR enhances hepatocyte engraftment and the integrated donor cell mass. Thus, preparative HIR could be potentially useful to augment hepatocyte transplantation.

Original languageEnglish (US)
Pages (from-to)258-267
Number of pages10
JournalHepatology
Volume49
Issue number1
DOIs
StatePublished - Mar 9 2009

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Hepatocytes
Transplantation
Liver
Dipeptidyl Peptidase 4
Kupffer Cells
Endothelial Cells
Hepatocyte Growth Factor
Macaca fascicularis
Hepatectomy
Rodentia
Swine
Carbon

ASJC Scopus subject areas

  • Hepatology

Cite this

Yamanouchi, K., Zhou, H., Roy-Chowdhury, N., Macaluso, F., Liu, L., Yamamoto, T., ... Guha, C. (2009). Hepatic irradiation augments engraftment of donor cells following hepatocyte transplantation. Hepatology, 49(1), 258-267. https://doi.org/10.1002/hep.22573

Hepatic irradiation augments engraftment of donor cells following hepatocyte transplantation. / Yamanouchi, Kosho; Zhou, Hongchao; Roy-Chowdhury, Namita; Macaluso, Frank; Liu, Liping; Yamamoto, Toshiyuki; Yannam, Govardhana Rao; Enke, Charles Arthur; Solberg, Timothy D.; Adelson, Anthony B.; Platt, Jeffrey L.; Fox, Ira J.; Roy-Chowdhury, Jayanta; Guha, Chandan.

In: Hepatology, Vol. 49, No. 1, 09.03.2009, p. 258-267.

Research output: Contribution to journalArticle

Yamanouchi, K, Zhou, H, Roy-Chowdhury, N, Macaluso, F, Liu, L, Yamamoto, T, Yannam, GR, Enke, CA, Solberg, TD, Adelson, AB, Platt, JL, Fox, IJ, Roy-Chowdhury, J & Guha, C 2009, 'Hepatic irradiation augments engraftment of donor cells following hepatocyte transplantation', Hepatology, vol. 49, no. 1, pp. 258-267. https://doi.org/10.1002/hep.22573
Yamanouchi K, Zhou H, Roy-Chowdhury N, Macaluso F, Liu L, Yamamoto T et al. Hepatic irradiation augments engraftment of donor cells following hepatocyte transplantation. Hepatology. 2009 Mar 9;49(1):258-267. https://doi.org/10.1002/hep.22573
Yamanouchi, Kosho ; Zhou, Hongchao ; Roy-Chowdhury, Namita ; Macaluso, Frank ; Liu, Liping ; Yamamoto, Toshiyuki ; Yannam, Govardhana Rao ; Enke, Charles Arthur ; Solberg, Timothy D. ; Adelson, Anthony B. ; Platt, Jeffrey L. ; Fox, Ira J. ; Roy-Chowdhury, Jayanta ; Guha, Chandan. / Hepatic irradiation augments engraftment of donor cells following hepatocyte transplantation. In: Hepatology. 2009 ; Vol. 49, No. 1. pp. 258-267.
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abstract = "Engraftment of donor hepatocytes is a critical step that determines the success of hepatocyte transplantation. Rapid and efficient integration of donor cells would enable prompt liver repopulation of these cells in response to selective proliferative stimuli offered by a preparative regimen. We have earlier demonstrated that hepatic irradiation (HIR) in combination with a variety of hepatotrophic growth signals, such as partial hepatectomy and hepatocyte growth factor, can be used as a preparative regimen for liver repopulation of transplanted hepatocytes. In this study, we investigated the effects of HIR on engraftment of transplanted dipeptidyl peptidase IV (DPPIV)-positive hepatocytes in congeneic DPPIV-deficient rats. HIR-induced apoptosis of hepatic sinusoidal endothelial cells (SEC) within 6 hours of HIR resulted in dehiscence of the SEC lining in 24 hours. Although there was no change of the number of Kupffer cells after HIR, colloidal carbon clearance decreased 24 hours post HIR, indicating a suppression of phagocytic function. DPPIV + donor cells were transplanted 24 hours after HIR (0-50 Gy). There was an HIR dose-dependent increase in the donor hepatocyte mass engrafted in the liver parenchyma. The number of viable transplanted hepatocytes present in hepatic sinusoids or integrated in the parenchyma was greater in the HIR-treated group at 3 and 7 days after transplantation compared with the sham controls. Finally, we validated these rodent studies in cynomolgus monkeys, demonstrating that a single 10-Gy dose of HIR was sufficient to enhance engraftment of donor porcine hepatocytes. These data indicate that transient disruption of the SEC barrier and inhibition of the phagocytic function of Kupffer cells by HIR enhances hepatocyte engraftment and the integrated donor cell mass. Thus, preparative HIR could be potentially useful to augment hepatocyte transplantation.",
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