Hematopathologic features of Epstein-Barr virus-induced human B-lymphoproliferation in mice with severe combined immunodeficiency

A model of lymphoproliferative diseases in immunocompromised patients

H. Nakamine, M. Okano, Y. Taguchi, Samuel Jay Pirruccello, J. R. Davis, K. W. Beisel, K. Kleveland, Warren G Sanger, R. R. Fordyce, D. T. Purtilo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The anatomical distribution, morphology, and clonality, of 'non-Hodgkin's lymphomas' in immunocompromised patients are usually distinctly different from NHL occurring in the general population. Mosier DE, Gulizia RJ, Baird SM, Wilson DB: Nature (London) 335:256, 1988 have described lymphoproliferative disease (LPD) of human B cell origin in mice with severe combined immunodeficiency (scid mice) after transfer of human peripheral blood mononuclear cells from Epstein-Barr virus-seropositive individuals. Reported herein is detailed information regarding the morphology, phenotypes, and clonality of LPD lesions in 10 of 18 scid mice that had developed LPD after transfer of peripheral blood mononuclear cells. These lesions were diffuse and monomorphic proliferations of immunoblastoid cells. They were invasive in their growth and often necrotic. Human B cell-related and activation-associated antigens were found on the LPD lesions, although the numbers of cells with the latter antigens were relatively small. Immunofixation electrophoresis for human immunoglobulins in sera of the majority of mice revealed oligoclonal populations, however, phenotypic and cytogenetic analyses showed no definite monoclonality. This scid mouse model is beneficial for understanding the early phases in the pathogenesis of LPD in immunocompromised patients.

Original languageEnglish (US)
Pages (from-to)389-399
Number of pages11
JournalLaboratory Investigation
Volume65
Issue number4
StatePublished - Jan 1 1991

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Severe Combined Immunodeficiency
Immunocompromised Host
Human Herpesvirus 4
Blood Cells
B-Lymphocytes
Antigens
Cytogenetic Analysis
Non-Hodgkin's Lymphoma
Population
Electrophoresis
Immunoglobulins
Cell Count
Cell Proliferation
Phenotype
Growth
Serum

Keywords

  • Cell transfer
  • In vivo model
  • Scid mouse

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Hematopathologic features of Epstein-Barr virus-induced human B-lymphoproliferation in mice with severe combined immunodeficiency : A model of lymphoproliferative diseases in immunocompromised patients. / Nakamine, H.; Okano, M.; Taguchi, Y.; Pirruccello, Samuel Jay; Davis, J. R.; Beisel, K. W.; Kleveland, K.; Sanger, Warren G; Fordyce, R. R.; Purtilo, D. T.

In: Laboratory Investigation, Vol. 65, No. 4, 01.01.1991, p. 389-399.

Research output: Contribution to journalArticle

Nakamine, H. ; Okano, M. ; Taguchi, Y. ; Pirruccello, Samuel Jay ; Davis, J. R. ; Beisel, K. W. ; Kleveland, K. ; Sanger, Warren G ; Fordyce, R. R. ; Purtilo, D. T. / Hematopathologic features of Epstein-Barr virus-induced human B-lymphoproliferation in mice with severe combined immunodeficiency : A model of lymphoproliferative diseases in immunocompromised patients. In: Laboratory Investigation. 1991 ; Vol. 65, No. 4. pp. 389-399.
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abstract = "The anatomical distribution, morphology, and clonality, of 'non-Hodgkin's lymphomas' in immunocompromised patients are usually distinctly different from NHL occurring in the general population. Mosier DE, Gulizia RJ, Baird SM, Wilson DB: Nature (London) 335:256, 1988 have described lymphoproliferative disease (LPD) of human B cell origin in mice with severe combined immunodeficiency (scid mice) after transfer of human peripheral blood mononuclear cells from Epstein-Barr virus-seropositive individuals. Reported herein is detailed information regarding the morphology, phenotypes, and clonality of LPD lesions in 10 of 18 scid mice that had developed LPD after transfer of peripheral blood mononuclear cells. These lesions were diffuse and monomorphic proliferations of immunoblastoid cells. They were invasive in their growth and often necrotic. Human B cell-related and activation-associated antigens were found on the LPD lesions, although the numbers of cells with the latter antigens were relatively small. Immunofixation electrophoresis for human immunoglobulins in sera of the majority of mice revealed oligoclonal populations, however, phenotypic and cytogenetic analyses showed no definite monoclonality. This scid mouse model is beneficial for understanding the early phases in the pathogenesis of LPD in immunocompromised patients.",
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