Hemagglutinin-based polyanhydride nanovaccines against H5N1 infuenza elicit protective virus neutralizing titers and cell-mediated immunity

Kathleen A. Ross, Hyelee Lyod, Wuwei Wu, Lucas Huntimer, Shaheen Ahmed, Anthony R. Sambol, Sccott Broderick, Zachary Flickinger, Krishna Rajan, Tatiana K Bronich, Surya Mallapragada, Michael J. Wannemuehler, Susan Carpenter, Balaji Narasimhan

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Abstract: H5N1 avian infuenza is a significant global concern with the potential to become the next pandemic threat. Recombinant subunit vaccines are an attractive alternative for pandemic vaccines compared to traditional vaccine technologies. In particular, polyanhydride nanoparticles encapsulating subunit proteins have been shown to enhance humoral and cellmediated immunity and provide protection upon lethal challenge. In this work, a recombinant H5 hemagglutinin trimer (H53) was produced and encapsulated into polyanhydride nanoparticles. The studies performed indicated that the recombinant H53 antigen was a robust immunogen. Immunizing mice with H53 encapsulated into polyanhydride nanoparticles induced high neutralizing antibody titers and enhanced CD4+ T cell recall responses in mice. Finally, the H53-based polyanhydride nanovaccine induced protective immunity against a low-pathogenic H5N1 viral challenge. Informatics analyses indicated that mice receiving the nanovaccine formulations and subsequently challenged with virus were similar to naç mice that were not challenged. The current studies provide a basis to further exploit the advantages of polyanhydride nanovaccines in pandemic scenarios.

Original languageEnglish (US)
Pages (from-to)229-243
Number of pages15
JournalInternational journal of nanomedicine
Volume10
DOIs
StatePublished - Jan 1 2015

Fingerprint

Polyanhydrides
Vaccines
Hemagglutinins
Viral Load
Viruses
Cellular Immunity
Nanoparticles
Pandemics
T-cells
Antigens
Antibodies
Synthetic Vaccines
Subunit Vaccines
Informatics
Proteins
Protein Subunits
Humoral Immunity
Neutralizing Antibodies
Immunity
Technology

Keywords

  • Nanoparticle
  • Neutralizing antibody
  • Polymer
  • Subunit
  • Vaccine

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

Hemagglutinin-based polyanhydride nanovaccines against H5N1 infuenza elicit protective virus neutralizing titers and cell-mediated immunity. / Ross, Kathleen A.; Lyod, Hyelee; Wu, Wuwei; Huntimer, Lucas; Ahmed, Shaheen; Sambol, Anthony R.; Broderick, Sccott; Flickinger, Zachary; Rajan, Krishna; Bronich, Tatiana K; Mallapragada, Surya; Wannemuehler, Michael J.; Carpenter, Susan; Narasimhan, Balaji.

In: International journal of nanomedicine, Vol. 10, 01.01.2015, p. 229-243.

Research output: Contribution to journalArticle

Ross, KA, Lyod, H, Wu, W, Huntimer, L, Ahmed, S, Sambol, AR, Broderick, S, Flickinger, Z, Rajan, K, Bronich, TK, Mallapragada, S, Wannemuehler, MJ, Carpenter, S & Narasimhan, B 2015, 'Hemagglutinin-based polyanhydride nanovaccines against H5N1 infuenza elicit protective virus neutralizing titers and cell-mediated immunity', International journal of nanomedicine, vol. 10, pp. 229-243. https://doi.org/10.2147/IJN.S72264
Ross, Kathleen A. ; Lyod, Hyelee ; Wu, Wuwei ; Huntimer, Lucas ; Ahmed, Shaheen ; Sambol, Anthony R. ; Broderick, Sccott ; Flickinger, Zachary ; Rajan, Krishna ; Bronich, Tatiana K ; Mallapragada, Surya ; Wannemuehler, Michael J. ; Carpenter, Susan ; Narasimhan, Balaji. / Hemagglutinin-based polyanhydride nanovaccines against H5N1 infuenza elicit protective virus neutralizing titers and cell-mediated immunity. In: International journal of nanomedicine. 2015 ; Vol. 10. pp. 229-243.
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