Helicobacter bilis colonization enhances susceptibility to typhlocolitis following an inflammatory trigger

Zhiping Liu, Amanda Ramer-Tait, Abigail L. Henderson, Cumhur Yusuf Demirkale, Dan Nettleton, Chong Wang, Jesse M. Hostetter, Albert E. Jergens, Michael J. Wannemuehler

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Aberrant mucosal immune responses to antigens of the resident microbiota are a significant cause of inflammatory bowel diseases (IBD), as are genetic and environmental factors. Previous work from our laboratory demonstrated that Helicobacter bilis colonization of immunocompetent, defined microbiota mice induced antigen-specific immune responses to the resident microbiota, yet these mice failed to develop colitis, suggesting that the immunological provocation induced by H. bilis alone was insufficient to induce disease. Aim: The purpose of this study was to test the hypothesis that the introduction of a bacterial provocateur such as H. bilis enhances the host's susceptibility to IBD following an inflammatory event. Methods: Defined microbiota (DM) mice colonized with H. bilis were administered low dose (1.5%) dextran sodium sulfate (DSS) in drinking water for 5 days followed by a 4-day restitution period. Severity of lesions was assessed grossly and microscopically. Differential expression of select mucosal genes and histopathologic lesions was characterized. Results: Helicobacter bilis colonization increased the severity of intestinal inflammation induced by an inflammatory trigger in the form of low-dose DSS. An analysis of the molecular and cellular mechanisms associated with H. bilis colonization revealed significant increases in expression of mucosal genes associated with lymphocyte activation and inflammatory cell chemotaxis as well as increased infiltration of mucosal macrophages and T cells in mice colonized with H. bilis prior to DSS treatment versus DSS treatment alone. Conclusions: These results indicate that prior colonization with H. bilis heightens the host's sensitivity to enteric inflammation by altering mucosal homeostasis and initiating immune cell activation and migration.

Original languageEnglish (US)
Pages (from-to)2838-2848
Number of pages11
JournalDigestive Diseases and Sciences
Volume56
Issue number10
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

Helicobacter
Dextran Sulfate
Microbiota
Histocompatibility Antigens Class II
Inflammatory Bowel Diseases
Inflammation
Mucosal Immunity
Chemotaxis
Colitis
Lymphocyte Activation
Drinking Water
Cell Movement
Homeostasis
Macrophages
T-Lymphocytes
Gene Expression
Therapeutics
Genes

Keywords

  • Activation
  • Chemotaxis
  • Colitis
  • Helicobacter bilis
  • Immune cells

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Helicobacter bilis colonization enhances susceptibility to typhlocolitis following an inflammatory trigger. / Liu, Zhiping; Ramer-Tait, Amanda; Henderson, Abigail L.; Demirkale, Cumhur Yusuf; Nettleton, Dan; Wang, Chong; Hostetter, Jesse M.; Jergens, Albert E.; Wannemuehler, Michael J.

In: Digestive Diseases and Sciences, Vol. 56, No. 10, 2011, p. 2838-2848.

Research output: Contribution to journalArticle

Liu, Z, Ramer-Tait, A, Henderson, AL, Demirkale, CY, Nettleton, D, Wang, C, Hostetter, JM, Jergens, AE & Wannemuehler, MJ 2011, 'Helicobacter bilis colonization enhances susceptibility to typhlocolitis following an inflammatory trigger', Digestive Diseases and Sciences, vol. 56, no. 10, pp. 2838-2848. https://doi.org/10.1007/s10620-011-1701-3
Liu, Zhiping ; Ramer-Tait, Amanda ; Henderson, Abigail L. ; Demirkale, Cumhur Yusuf ; Nettleton, Dan ; Wang, Chong ; Hostetter, Jesse M. ; Jergens, Albert E. ; Wannemuehler, Michael J. / Helicobacter bilis colonization enhances susceptibility to typhlocolitis following an inflammatory trigger. In: Digestive Diseases and Sciences. 2011 ; Vol. 56, No. 10. pp. 2838-2848.
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AU - Ramer-Tait, Amanda

AU - Henderson, Abigail L.

AU - Demirkale, Cumhur Yusuf

AU - Nettleton, Dan

AU - Wang, Chong

AU - Hostetter, Jesse M.

AU - Jergens, Albert E.

AU - Wannemuehler, Michael J.

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N2 - Background: Aberrant mucosal immune responses to antigens of the resident microbiota are a significant cause of inflammatory bowel diseases (IBD), as are genetic and environmental factors. Previous work from our laboratory demonstrated that Helicobacter bilis colonization of immunocompetent, defined microbiota mice induced antigen-specific immune responses to the resident microbiota, yet these mice failed to develop colitis, suggesting that the immunological provocation induced by H. bilis alone was insufficient to induce disease. Aim: The purpose of this study was to test the hypothesis that the introduction of a bacterial provocateur such as H. bilis enhances the host's susceptibility to IBD following an inflammatory event. Methods: Defined microbiota (DM) mice colonized with H. bilis were administered low dose (1.5%) dextran sodium sulfate (DSS) in drinking water for 5 days followed by a 4-day restitution period. Severity of lesions was assessed grossly and microscopically. Differential expression of select mucosal genes and histopathologic lesions was characterized. Results: Helicobacter bilis colonization increased the severity of intestinal inflammation induced by an inflammatory trigger in the form of low-dose DSS. An analysis of the molecular and cellular mechanisms associated with H. bilis colonization revealed significant increases in expression of mucosal genes associated with lymphocyte activation and inflammatory cell chemotaxis as well as increased infiltration of mucosal macrophages and T cells in mice colonized with H. bilis prior to DSS treatment versus DSS treatment alone. Conclusions: These results indicate that prior colonization with H. bilis heightens the host's sensitivity to enteric inflammation by altering mucosal homeostasis and initiating immune cell activation and migration.

AB - Background: Aberrant mucosal immune responses to antigens of the resident microbiota are a significant cause of inflammatory bowel diseases (IBD), as are genetic and environmental factors. Previous work from our laboratory demonstrated that Helicobacter bilis colonization of immunocompetent, defined microbiota mice induced antigen-specific immune responses to the resident microbiota, yet these mice failed to develop colitis, suggesting that the immunological provocation induced by H. bilis alone was insufficient to induce disease. Aim: The purpose of this study was to test the hypothesis that the introduction of a bacterial provocateur such as H. bilis enhances the host's susceptibility to IBD following an inflammatory event. Methods: Defined microbiota (DM) mice colonized with H. bilis were administered low dose (1.5%) dextran sodium sulfate (DSS) in drinking water for 5 days followed by a 4-day restitution period. Severity of lesions was assessed grossly and microscopically. Differential expression of select mucosal genes and histopathologic lesions was characterized. Results: Helicobacter bilis colonization increased the severity of intestinal inflammation induced by an inflammatory trigger in the form of low-dose DSS. An analysis of the molecular and cellular mechanisms associated with H. bilis colonization revealed significant increases in expression of mucosal genes associated with lymphocyte activation and inflammatory cell chemotaxis as well as increased infiltration of mucosal macrophages and T cells in mice colonized with H. bilis prior to DSS treatment versus DSS treatment alone. Conclusions: These results indicate that prior colonization with H. bilis heightens the host's sensitivity to enteric inflammation by altering mucosal homeostasis and initiating immune cell activation and migration.

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