Heat-Stress Response of Maize Mitochondria

Adrian A. Lund, Paul H. Blum, Dinakar Bhattramakki, Thomas E. Elthon

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We have identified maize (Zea mays L. inbred B73) mitochondrial homologs of the Escherichia coli molecular chaperones DnaK (HSP70) and GroEL (cpn60) using two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblots. During heat stress (42°C for 4 h), levels of HSP70 and cpn60 proteins did not change significantly. In contrast, levels of two 22-kD proteins increased dramatically (HSP22). Monoclonal antibodies were developed to maize HSP70, cpn60, and HSP22. The monoclonal antibodies were characterized with regard to their cross-reactivity to chloroplastic, cytosolic, and mitochondrial fractions, and to different plant species. Expression of mitochondrial HSP22 was evaluated with regard to induction temperature, time required for induction, and time required for degradation upon relief of stress. Maximal HSP22 expression occurred in etiolated seedling mitochondria after 5 h of a +13°C heat stress. Upon relief of heat stress, the HSP22 proteins disappeared with a half-life of about 4 h and were undetectable after 21 h of recovery. Under continuous heat-stress conditions, the level of HSP22 remained high. A cDNA for maize mitochondrial HSP22 was cloned and extended to full length with sequences from an expressed sequence tag database. Sequence analysis indicated that HSP22 is a member of the plant small heat-shock protein superfamily.

Original languageEnglish (US)
Pages (from-to)1097-1110
Number of pages14
JournalPlant physiology
Volume116
Issue number3
DOIs
StatePublished - Mar 1998

Fingerprint

Heat-Shock Response
Zea mays
heat stress
Mitochondria
mitochondria
Hot Temperature
corn
monoclonal antibodies
Monoclonal Antibodies
Small Heat-Shock Proteins
Plant Proteins
molecular chaperones
Molecular Chaperones
proteins
Expressed Sequence Tags
two-dimensional gel electrophoresis
Heat-Shock Proteins
expressed sequence tags
Seedlings
heat shock proteins

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Lund, A. A., Blum, P. H., Bhattramakki, D., & Elthon, T. E. (1998). Heat-Stress Response of Maize Mitochondria. Plant physiology, 116(3), 1097-1110. https://doi.org/10.1104/pp.116.3.1097

Heat-Stress Response of Maize Mitochondria. / Lund, Adrian A.; Blum, Paul H.; Bhattramakki, Dinakar; Elthon, Thomas E.

In: Plant physiology, Vol. 116, No. 3, 03.1998, p. 1097-1110.

Research output: Contribution to journalArticle

Lund, AA, Blum, PH, Bhattramakki, D & Elthon, TE 1998, 'Heat-Stress Response of Maize Mitochondria', Plant physiology, vol. 116, no. 3, pp. 1097-1110. https://doi.org/10.1104/pp.116.3.1097
Lund, Adrian A. ; Blum, Paul H. ; Bhattramakki, Dinakar ; Elthon, Thomas E. / Heat-Stress Response of Maize Mitochondria. In: Plant physiology. 1998 ; Vol. 116, No. 3. pp. 1097-1110.
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