Heat-stress induced synthesis of chloroplast protein synthesis elongation factor (EF-Tu) in a heat-tolerant maize line

Shailendra K. Bhadula, Thomas E Elthon, Jeffrey E. Habben, Timothy G. Helentjaris, Shuping Jiao, Zoran Ristic

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

A heat-tolerant maize (Zea mays L.) line, ZPBL 1304, synthesizes a unique set of five heat-shock polypeptides of 45 kDa. Previous studies suggested that these polypeptides might play a role in the development of thermotolerance in maize (Ristic et al., 1996, J. Plant Physiol. 149:424-432; Ristic et al., 1998, J. Plant Physiol. 153:497-505). In the present study, we isolated these polypeptides, sequenced them, and investigated their subcellular distribution and origin. Of the five polypeptides of 45 kDa, three polypeptides, including the two most abundant ones, yielded amino acid sequences similar to the chloroplast and bacterial protein synthesis elongation factor (EF-Tu). This was further confirmed using an antibody raised against maize EF-Tu, which showed a very strong reaction with the 45-kDa heat-shock protein(s). Studies on subcellular distribution and origin revealed that the 45-kDa polypeptides were localized to the chloroplasts, and were likely of nuclear origin. A full-length maize EF-Tu cDNA (Zmeftul), previously isolated from the B73 line of maize, was used as a probe for northern blot analysis of RNA extracted from the ZPBL 1304 maize line (the nucleotide and deduced amino acid sequences of Zmeftul are 88% identical to the rice EF-Tu sequence). Northern blots showed a 1.85-fold increase in steady-state levels of EF-Tu mRNA during heat stress. An increase in EF-Tu transcript levels during heat stress was accompanied by increased levels of the EF-Tu protein. Isolated chloroplasts from heat-stressed plants also had higher levels of EF-Tu as compared to control chloroplasts. The maize EF-Tu polypeptides showed > 80% sequence similarity with the bacterial EF-Tu, which has recently been shown to function as a molecular chaperone and to play a role in the protection of other proteins from thermal denaturatìon (Caldas et al., 1998, J. Biol. Chem. 273:11478-11482). It is hypothesized that chloroplast EF-Tu of the ZPBL 1304 maize line plays an important role in the development of thermotolerance.

Original languageEnglish (US)
Pages (from-to)359-366
Number of pages8
JournalPlanta
Volume212
Issue number3
DOIs
StatePublished - Mar 12 2001

Fingerprint

Peptide Elongation Factor Tu
Chloroplast Proteins
Zea mays
heat stress
chloroplasts
protein synthesis
Hot Temperature
polypeptides
heat
synthesis
corn
Peptides
Chloroplasts
heat tolerance
Northern blotting
amino acid sequences
Northern Blotting
bacterial proteins
molecular chaperones
Amino Acid Sequence

Keywords

  • Chloroplast protein synthesis elongation factor (EF-Tu)
  • Heat stress
  • Heat tolerance
  • Heat-shock protein
  • Zea mays (heat stress)

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

Bhadula, S. K., Elthon, T. E., Habben, J. E., Helentjaris, T. G., Jiao, S., & Ristic, Z. (2001). Heat-stress induced synthesis of chloroplast protein synthesis elongation factor (EF-Tu) in a heat-tolerant maize line. Planta, 212(3), 359-366. https://doi.org/10.1007/s004250000416

Heat-stress induced synthesis of chloroplast protein synthesis elongation factor (EF-Tu) in a heat-tolerant maize line. / Bhadula, Shailendra K.; Elthon, Thomas E; Habben, Jeffrey E.; Helentjaris, Timothy G.; Jiao, Shuping; Ristic, Zoran.

In: Planta, Vol. 212, No. 3, 12.03.2001, p. 359-366.

Research output: Contribution to journalArticle

Bhadula, SK, Elthon, TE, Habben, JE, Helentjaris, TG, Jiao, S & Ristic, Z 2001, 'Heat-stress induced synthesis of chloroplast protein synthesis elongation factor (EF-Tu) in a heat-tolerant maize line', Planta, vol. 212, no. 3, pp. 359-366. https://doi.org/10.1007/s004250000416
Bhadula, Shailendra K. ; Elthon, Thomas E ; Habben, Jeffrey E. ; Helentjaris, Timothy G. ; Jiao, Shuping ; Ristic, Zoran. / Heat-stress induced synthesis of chloroplast protein synthesis elongation factor (EF-Tu) in a heat-tolerant maize line. In: Planta. 2001 ; Vol. 212, No. 3. pp. 359-366.
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abstract = "A heat-tolerant maize (Zea mays L.) line, ZPBL 1304, synthesizes a unique set of five heat-shock polypeptides of 45 kDa. Previous studies suggested that these polypeptides might play a role in the development of thermotolerance in maize (Ristic et al., 1996, J. Plant Physiol. 149:424-432; Ristic et al., 1998, J. Plant Physiol. 153:497-505). In the present study, we isolated these polypeptides, sequenced them, and investigated their subcellular distribution and origin. Of the five polypeptides of 45 kDa, three polypeptides, including the two most abundant ones, yielded amino acid sequences similar to the chloroplast and bacterial protein synthesis elongation factor (EF-Tu). This was further confirmed using an antibody raised against maize EF-Tu, which showed a very strong reaction with the 45-kDa heat-shock protein(s). Studies on subcellular distribution and origin revealed that the 45-kDa polypeptides were localized to the chloroplasts, and were likely of nuclear origin. A full-length maize EF-Tu cDNA (Zmeftul), previously isolated from the B73 line of maize, was used as a probe for northern blot analysis of RNA extracted from the ZPBL 1304 maize line (the nucleotide and deduced amino acid sequences of Zmeftul are 88{\%} identical to the rice EF-Tu sequence). Northern blots showed a 1.85-fold increase in steady-state levels of EF-Tu mRNA during heat stress. An increase in EF-Tu transcript levels during heat stress was accompanied by increased levels of the EF-Tu protein. Isolated chloroplasts from heat-stressed plants also had higher levels of EF-Tu as compared to control chloroplasts. The maize EF-Tu polypeptides showed > 80{\%} sequence similarity with the bacterial EF-Tu, which has recently been shown to function as a molecular chaperone and to play a role in the protection of other proteins from thermal denaturat{\`i}on (Caldas et al., 1998, J. Biol. Chem. 273:11478-11482). It is hypothesized that chloroplast EF-Tu of the ZPBL 1304 maize line plays an important role in the development of thermotolerance.",
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