Heat shock applied early in sporulation affects heat resistance of Bacillus megaterium spores

M. Sedlak, V. Vinter, J. Adamec, J. Vohradsky, Z. Voburka, J. Chaloupka

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Cells of Bacillus megaterium 27 were challenged by a 30-min heat shock at 45°C during various sporulation stages and then shifted back to a temperature permissive for sporulation (27°C), at which they developed spores. Heat shock applied at 120 min after the end of the exponential phase induced synthesis of heat shock proteins (HSPs) in the sporangia and delayed the inactivation of spores at 85°C. Several HSPs, mainly HSP 70, could be detected in the cytoplasm of these spores. An analogous HSP, the main HSP induced by increased temperature during growth, belongs to the GroEL group according to its N-terminal sequence. The identity of this protein was confirmed by Western blot (immunoblot) analysis with polyclonal antibodies against B. subtilis GroEL. Sporangia treated by heat shock immediately or 240 min after exponential phase also synthesized HSPs, but none of them could be detected in the spores in an appreciable amount. These spores showed only a slightly increased heat resistance.

Original languageEnglish (US)
Pages (from-to)8049-8052
Number of pages4
JournalJournal of bacteriology
Volume175
Issue number24
DOIs
StatePublished - Jan 1 1993

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Bacillus megaterium
Heat-Shock Proteins
Spores
Shock
Hot Temperature
Sporangia
Western Blotting
Temperature
HSP70 Heat-Shock Proteins
Cytoplasm
Antibodies
Growth
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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Heat shock applied early in sporulation affects heat resistance of Bacillus megaterium spores. / Sedlak, M.; Vinter, V.; Adamec, J.; Vohradsky, J.; Voburka, Z.; Chaloupka, J.

In: Journal of bacteriology, Vol. 175, No. 24, 01.01.1993, p. 8049-8052.

Research output: Contribution to journalArticle

Sedlak, M. ; Vinter, V. ; Adamec, J. ; Vohradsky, J. ; Voburka, Z. ; Chaloupka, J. / Heat shock applied early in sporulation affects heat resistance of Bacillus megaterium spores. In: Journal of bacteriology. 1993 ; Vol. 175, No. 24. pp. 8049-8052.
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