Heart failure-induced changes of voltage-gated Ca2+ channels and cell excitability in rat cardiac postganglionic neurons

Huiyin Tu, Jinxu Liu, Dongze Zhang, Hong Zheng, Kaushik P Patel, Kurtis G. Cornish, Wei Zhong Wang, Robert Leo Muelleman, Yulong Li

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Chronic heart failure (CHF) is characterized by decreased cardiac parasympathetic and increased cardiac sympathetic nerve activity. This autonomic imbalance increases the risk of arrhythmias and sudden death in patients with CHF. We hypothesized that the molecular and cellular alterations of cardiac postganglionic parasympathetic (CPP) neurons located in the intracardiac ganglia and sympathetic (CPS) neurons located in the stellate ganglia (SG) possibly link to the cardiac autonomic imbalance in CHF. Rat CHF was induced by left coronary artery ligation. Single-cell realtime PCR and immunofluorescent data showed that L (Cav1.2 and Cav1.3), P/Q (Cav2.1), N (Cav2.2), and R (Cav2.3) types of Ca2+ channels were expressed in CPP and CPS neurons, but CHF decreased the mRNA and protein expression of only the N-type Ca2+ channels in CPP neurons, and it did not affect mRNA and protein expression of all Ca2+ channel subtypes in the CPS neurons. Patch-clamp recording confirmed that CHF reduced N-type Ca2+ currents and cell excitability in the CPP neurons and enhanced N-type Ca2+ currents and cell excitability in the CPS neurons. N-type Ca2+ channel blocker (1 μM ω-conotoxin GVIA) lowered Ca2+ currents and cell excitability in the CPP and CPS neurons from sham-operated and CHF rats. These results suggest that CHF reduces the N-type Ca2+ channel currents and cell excitability in the CPP neurons and enhances the N-type Ca2+ currents and cell excitability in the CPS neurons, which may contribute to the cardiac autonomic imbalance in CHF.

Original languageEnglish (US)
Pages (from-to)C132-C142
JournalAmerican Journal of Physiology - Cell Physiology
Volume306
Issue number2
DOIs
StatePublished - Jan 15 2014

Fingerprint

Heart Failure
Neurons
Stellate Ganglion
Messenger RNA
Sympathetic Ganglia
Sudden Death
Ligation
Cardiac Arrhythmias
Coronary Vessels
Proteins
Polymerase Chain Reaction

Keywords

  • Action potential
  • Autonomic dysfunction
  • Cardiac ganglia
  • Ion channels

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Heart failure-induced changes of voltage-gated Ca2+ channels and cell excitability in rat cardiac postganglionic neurons. / Tu, Huiyin; Liu, Jinxu; Zhang, Dongze; Zheng, Hong; Patel, Kaushik P; Cornish, Kurtis G.; Wang, Wei Zhong; Muelleman, Robert Leo; Li, Yulong.

In: American Journal of Physiology - Cell Physiology, Vol. 306, No. 2, 15.01.2014, p. C132-C142.

Research output: Contribution to journalArticle

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