Healthcare workers occupationally exposed to ionizing radiation exhibit altered levels of inflammatory cytokines and redox parameters

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Abstract

Studies have shown an increased risk for a variety of cancers, specifically brain cancer, in healthcare workers occupationally exposed to ionizing radiation. Although the mechanisms mediating these phenomena are not fully understood, ionizing radiation-mediated elevated levels of reactive oxygen species (ROS), oxidative DNA damage, and immune modulation are likely involved. A group of 20 radiation exposed workers and 40 sex-and age-matched non-exposed control subjects were recruited for the study. We measured superoxide (O2) levels in whole blood of healthcare workers and all other measurements of cytokines, oxidative DNA damage, extracellular superoxide dismutase (EcSOD) activity and reduced/oxidized glutathione ratio (GSH/GSSG) in plasma. Levels of O2 were significantly higher in radiation exposed workers compared to control. Similarly, a significant increase in the levels of interleukin (IL)-6, IL-1α and macrophage inflammatory protein (MIP)-1α in radiation exposed workers compared to control was observed, while there was no significance difference in the other 27 screened cytokines. A significant positive correlation was found between MIP-1α and O2 levels with no correlation in either IL-6 or IL-1α. Further, a dose-dependent relationship with significant O2 production and immune alterations in radiation exposed workers was demonstrated. There was no statistical difference between the groups in terms of oxidative DNA damage, GSH/GSSG levels, or EcSOD activity. Although the biologic significance of cytokines alterations in radiation exposed workers is unclear, further studies are needed for determining the underlying mechanism of their elevation.

Original languageEnglish (US)
Article number12
JournalAntioxidants
Volume8
Issue number1
DOIs
StatePublished - Jan 1 2019

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Ionizing radiation
Ionizing Radiation
Oxidation-Reduction
Glutathione Disulfide
Radiation
Cytokines
Delivery of Health Care
DNA Damage
Macrophage Inflammatory Proteins
Interleukin-1
Brain Neoplasms
Superoxide Dismutase
Interleukin-6
DNA
Sex Workers
Superoxides
Glutathione
Reactive Oxygen Species
Brain
Blood

Keywords

  • DNA oxidation
  • EcSOD
  • Glutathione
  • Inflammatory cytokines
  • O
  • Oxidative stress

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

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title = "Healthcare workers occupationally exposed to ionizing radiation exhibit altered levels of inflammatory cytokines and redox parameters",
abstract = "Studies have shown an increased risk for a variety of cancers, specifically brain cancer, in healthcare workers occupationally exposed to ionizing radiation. Although the mechanisms mediating these phenomena are not fully understood, ionizing radiation-mediated elevated levels of reactive oxygen species (ROS), oxidative DNA damage, and immune modulation are likely involved. A group of 20 radiation exposed workers and 40 sex-and age-matched non-exposed control subjects were recruited for the study. We measured superoxide (O2 •−) levels in whole blood of healthcare workers and all other measurements of cytokines, oxidative DNA damage, extracellular superoxide dismutase (EcSOD) activity and reduced/oxidized glutathione ratio (GSH/GSSG) in plasma. Levels of O2 •− were significantly higher in radiation exposed workers compared to control. Similarly, a significant increase in the levels of interleukin (IL)-6, IL-1α and macrophage inflammatory protein (MIP)-1α in radiation exposed workers compared to control was observed, while there was no significance difference in the other 27 screened cytokines. A significant positive correlation was found between MIP-1α and O2 •− levels with no correlation in either IL-6 or IL-1α. Further, a dose-dependent relationship with significant O2 •− production and immune alterations in radiation exposed workers was demonstrated. There was no statistical difference between the groups in terms of oxidative DNA damage, GSH/GSSG levels, or EcSOD activity. Although the biologic significance of cytokines alterations in radiation exposed workers is unclear, further studies are needed for determining the underlying mechanism of their elevation.",
keywords = "DNA oxidation, EcSOD, Glutathione, Inflammatory cytokines, O, Oxidative stress",
author = "Ahmad, {Iman M} and Abdalla, {Maher Y} and Moore, {Tiffany A} and Bartenhagen, {Lisa Ann} and Adam Case and Zimmerman, {Matthew C}",
year = "2019",
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doi = "10.3390/antiox8010012",
language = "English (US)",
volume = "8",
journal = "Antioxidants",
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T1 - Healthcare workers occupationally exposed to ionizing radiation exhibit altered levels of inflammatory cytokines and redox parameters

AU - Ahmad, Iman M

AU - Abdalla, Maher Y

AU - Moore, Tiffany A

AU - Bartenhagen, Lisa Ann

AU - Case, Adam

AU - Zimmerman, Matthew C

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Studies have shown an increased risk for a variety of cancers, specifically brain cancer, in healthcare workers occupationally exposed to ionizing radiation. Although the mechanisms mediating these phenomena are not fully understood, ionizing radiation-mediated elevated levels of reactive oxygen species (ROS), oxidative DNA damage, and immune modulation are likely involved. A group of 20 radiation exposed workers and 40 sex-and age-matched non-exposed control subjects were recruited for the study. We measured superoxide (O2 •−) levels in whole blood of healthcare workers and all other measurements of cytokines, oxidative DNA damage, extracellular superoxide dismutase (EcSOD) activity and reduced/oxidized glutathione ratio (GSH/GSSG) in plasma. Levels of O2 •− were significantly higher in radiation exposed workers compared to control. Similarly, a significant increase in the levels of interleukin (IL)-6, IL-1α and macrophage inflammatory protein (MIP)-1α in radiation exposed workers compared to control was observed, while there was no significance difference in the other 27 screened cytokines. A significant positive correlation was found between MIP-1α and O2 •− levels with no correlation in either IL-6 or IL-1α. Further, a dose-dependent relationship with significant O2 •− production and immune alterations in radiation exposed workers was demonstrated. There was no statistical difference between the groups in terms of oxidative DNA damage, GSH/GSSG levels, or EcSOD activity. Although the biologic significance of cytokines alterations in radiation exposed workers is unclear, further studies are needed for determining the underlying mechanism of their elevation.

AB - Studies have shown an increased risk for a variety of cancers, specifically brain cancer, in healthcare workers occupationally exposed to ionizing radiation. Although the mechanisms mediating these phenomena are not fully understood, ionizing radiation-mediated elevated levels of reactive oxygen species (ROS), oxidative DNA damage, and immune modulation are likely involved. A group of 20 radiation exposed workers and 40 sex-and age-matched non-exposed control subjects were recruited for the study. We measured superoxide (O2 •−) levels in whole blood of healthcare workers and all other measurements of cytokines, oxidative DNA damage, extracellular superoxide dismutase (EcSOD) activity and reduced/oxidized glutathione ratio (GSH/GSSG) in plasma. Levels of O2 •− were significantly higher in radiation exposed workers compared to control. Similarly, a significant increase in the levels of interleukin (IL)-6, IL-1α and macrophage inflammatory protein (MIP)-1α in radiation exposed workers compared to control was observed, while there was no significance difference in the other 27 screened cytokines. A significant positive correlation was found between MIP-1α and O2 •− levels with no correlation in either IL-6 or IL-1α. Further, a dose-dependent relationship with significant O2 •− production and immune alterations in radiation exposed workers was demonstrated. There was no statistical difference between the groups in terms of oxidative DNA damage, GSH/GSSG levels, or EcSOD activity. Although the biologic significance of cytokines alterations in radiation exposed workers is unclear, further studies are needed for determining the underlying mechanism of their elevation.

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KW - Inflammatory cytokines

KW - O

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