Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in frozen human plasma

John A. Bowden, Alan Heckert, Candice Z. Ulmer, Christina M. Jones, Jeremy P. Koelmel, Laila Abdullah, Linda Ahonen, Yazen Alnouti, Aaron M. Armando, John M. Asara, Takeshi Bamba, John R. Barr, Jonas Bergquist, Christoph H. Borchers, Joost Brandsma, Susanne B. Breitkopf, Tomas Cajka, Amaury Cazenave-Gassiot, Antonio Checa, Michelle A. CinelRomain A. Colas, Serge Cremers, Edward A. Dennis, James E. Evans, Alexander Fauland, Oliver Fiehn, Michael S. Gardner, Timothy J. Garrett, Katherine H. Gotlinger, Jun Han, Yingying Huang, Aveline Huipeng Neo, Tuulia Hyötyläinen, Yoshihiro Izumi, Hongfeng Jiang, Houli Jiang, Jiang Jiang, Maureen Kachman, Reiko Kiyonami, Kristaps Klavins, Christian Klose, Harald C. Köfeler, Johan Kolmert, Therese Koal, Grielof Koster, Zsuzsanna Kuklenyik, Irwin J. Kurland, Michael Leadley, Karen Lin, Krishna Rao Maddipati, Danielle McDougall, Peter J. Meikle, Natalie A. Mellett, Cian Monnin, M. Arthur Moseley, Renu Nandakumar, Matej Oresic, Rainey Patterson, David Peake, Jason S. Pierce, Martin Post, Anthony D. Postle, Rebecca Pugh, Yunping Qiu, Oswald Quehenberger, Parsram Ramrup, Jon Rees, Barbara Rembiesa, Denis Reynaud, Mary R. Roth, Susanne Sales, Kai Schuhmann, Michal Laniado Schwartzman, Charles N. Serhan, Andrej Shevchenko, Stephen E. Somerville, Lisa St John-Williams, Michal A. Surma, Hiroaki Takeda, Rhishikesh Thakare, J. Will Thompson, Federico Torta, Alexander Triebl, Martin Trötzmüller, S. J.Kumari Ubhayasekera, Dajana Vuckovic, Jacquelyn M. Weir, Ruth Welti, Markus R. Wenk, Craig E. Wheelock, Libin Yao, Min Yuan, Xueqing Heather Zhao, Senlin Zhou

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950- Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra-And interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium. While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.

Original languageEnglish (US)
Pages (from-to)2275-2288
Number of pages14
JournalJournal of Lipid Research
Volume58
Issue number12
DOIs
StatePublished - Jan 1 2017

Fingerprint

Plasma (human)
Metabolites
Lipids
Workflow
Consensus
Benchmarking
Diagnostic Self Evaluation
Quality Control
Uncertainty
Quality control
Chemical analysis

Keywords

  • Fatty acyls
  • Glycerolipids
  • Lipids
  • National institute of standards and technology
  • Phospholipids
  • Quality control
  • Quantitation
  • Sphingolipids
  • Standard reference material 1950
  • Sterols

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Bowden, J. A., Heckert, A., Ulmer, C. Z., Jones, C. M., Koelmel, J. P., Abdullah, L., ... Zhou, S. (2017). Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in frozen human plasma. Journal of Lipid Research, 58(12), 2275-2288. https://doi.org/10.1194/jlr.M079012

Harmonizing lipidomics : NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in frozen human plasma. / Bowden, John A.; Heckert, Alan; Ulmer, Candice Z.; Jones, Christina M.; Koelmel, Jeremy P.; Abdullah, Laila; Ahonen, Linda; Alnouti, Yazen; Armando, Aaron M.; Asara, John M.; Bamba, Takeshi; Barr, John R.; Bergquist, Jonas; Borchers, Christoph H.; Brandsma, Joost; Breitkopf, Susanne B.; Cajka, Tomas; Cazenave-Gassiot, Amaury; Checa, Antonio; Cinel, Michelle A.; Colas, Romain A.; Cremers, Serge; Dennis, Edward A.; Evans, James E.; Fauland, Alexander; Fiehn, Oliver; Gardner, Michael S.; Garrett, Timothy J.; Gotlinger, Katherine H.; Han, Jun; Huang, Yingying; Neo, Aveline Huipeng; Hyötyläinen, Tuulia; Izumi, Yoshihiro; Jiang, Hongfeng; Jiang, Houli; Jiang, Jiang; Kachman, Maureen; Kiyonami, Reiko; Klavins, Kristaps; Klose, Christian; Köfeler, Harald C.; Kolmert, Johan; Koal, Therese; Koster, Grielof; Kuklenyik, Zsuzsanna; Kurland, Irwin J.; Leadley, Michael; Lin, Karen; Maddipati, Krishna Rao; McDougall, Danielle; Meikle, Peter J.; Mellett, Natalie A.; Monnin, Cian; Moseley, M. Arthur; Nandakumar, Renu; Oresic, Matej; Patterson, Rainey; Peake, David; Pierce, Jason S.; Post, Martin; Postle, Anthony D.; Pugh, Rebecca; Qiu, Yunping; Quehenberger, Oswald; Ramrup, Parsram; Rees, Jon; Rembiesa, Barbara; Reynaud, Denis; Roth, Mary R.; Sales, Susanne; Schuhmann, Kai; Schwartzman, Michal Laniado; Serhan, Charles N.; Shevchenko, Andrej; Somerville, Stephen E.; St John-Williams, Lisa; Surma, Michal A.; Takeda, Hiroaki; Thakare, Rhishikesh; Thompson, J. Will; Torta, Federico; Triebl, Alexander; Trötzmüller, Martin; Ubhayasekera, S. J.Kumari; Vuckovic, Dajana; Weir, Jacquelyn M.; Welti, Ruth; Wenk, Markus R.; Wheelock, Craig E.; Yao, Libin; Yuan, Min; Zhao, Xueqing Heather; Zhou, Senlin.

In: Journal of Lipid Research, Vol. 58, No. 12, 01.01.2017, p. 2275-2288.

Research output: Contribution to journalArticle

Bowden, JA, Heckert, A, Ulmer, CZ, Jones, CM, Koelmel, JP, Abdullah, L, Ahonen, L, Alnouti, Y, Armando, AM, Asara, JM, Bamba, T, Barr, JR, Bergquist, J, Borchers, CH, Brandsma, J, Breitkopf, SB, Cajka, T, Cazenave-Gassiot, A, Checa, A, Cinel, MA, Colas, RA, Cremers, S, Dennis, EA, Evans, JE, Fauland, A, Fiehn, O, Gardner, MS, Garrett, TJ, Gotlinger, KH, Han, J, Huang, Y, Neo, AH, Hyötyläinen, T, Izumi, Y, Jiang, H, Jiang, H, Jiang, J, Kachman, M, Kiyonami, R, Klavins, K, Klose, C, Köfeler, HC, Kolmert, J, Koal, T, Koster, G, Kuklenyik, Z, Kurland, IJ, Leadley, M, Lin, K, Maddipati, KR, McDougall, D, Meikle, PJ, Mellett, NA, Monnin, C, Moseley, MA, Nandakumar, R, Oresic, M, Patterson, R, Peake, D, Pierce, JS, Post, M, Postle, AD, Pugh, R, Qiu, Y, Quehenberger, O, Ramrup, P, Rees, J, Rembiesa, B, Reynaud, D, Roth, MR, Sales, S, Schuhmann, K, Schwartzman, ML, Serhan, CN, Shevchenko, A, Somerville, SE, St John-Williams, L, Surma, MA, Takeda, H, Thakare, R, Thompson, JW, Torta, F, Triebl, A, Trötzmüller, M, Ubhayasekera, SJK, Vuckovic, D, Weir, JM, Welti, R, Wenk, MR, Wheelock, CE, Yao, L, Yuan, M, Zhao, XH & Zhou, S 2017, 'Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in frozen human plasma', Journal of Lipid Research, vol. 58, no. 12, pp. 2275-2288. https://doi.org/10.1194/jlr.M079012
Bowden, John A. ; Heckert, Alan ; Ulmer, Candice Z. ; Jones, Christina M. ; Koelmel, Jeremy P. ; Abdullah, Laila ; Ahonen, Linda ; Alnouti, Yazen ; Armando, Aaron M. ; Asara, John M. ; Bamba, Takeshi ; Barr, John R. ; Bergquist, Jonas ; Borchers, Christoph H. ; Brandsma, Joost ; Breitkopf, Susanne B. ; Cajka, Tomas ; Cazenave-Gassiot, Amaury ; Checa, Antonio ; Cinel, Michelle A. ; Colas, Romain A. ; Cremers, Serge ; Dennis, Edward A. ; Evans, James E. ; Fauland, Alexander ; Fiehn, Oliver ; Gardner, Michael S. ; Garrett, Timothy J. ; Gotlinger, Katherine H. ; Han, Jun ; Huang, Yingying ; Neo, Aveline Huipeng ; Hyötyläinen, Tuulia ; Izumi, Yoshihiro ; Jiang, Hongfeng ; Jiang, Houli ; Jiang, Jiang ; Kachman, Maureen ; Kiyonami, Reiko ; Klavins, Kristaps ; Klose, Christian ; Köfeler, Harald C. ; Kolmert, Johan ; Koal, Therese ; Koster, Grielof ; Kuklenyik, Zsuzsanna ; Kurland, Irwin J. ; Leadley, Michael ; Lin, Karen ; Maddipati, Krishna Rao ; McDougall, Danielle ; Meikle, Peter J. ; Mellett, Natalie A. ; Monnin, Cian ; Moseley, M. Arthur ; Nandakumar, Renu ; Oresic, Matej ; Patterson, Rainey ; Peake, David ; Pierce, Jason S. ; Post, Martin ; Postle, Anthony D. ; Pugh, Rebecca ; Qiu, Yunping ; Quehenberger, Oswald ; Ramrup, Parsram ; Rees, Jon ; Rembiesa, Barbara ; Reynaud, Denis ; Roth, Mary R. ; Sales, Susanne ; Schuhmann, Kai ; Schwartzman, Michal Laniado ; Serhan, Charles N. ; Shevchenko, Andrej ; Somerville, Stephen E. ; St John-Williams, Lisa ; Surma, Michal A. ; Takeda, Hiroaki ; Thakare, Rhishikesh ; Thompson, J. Will ; Torta, Federico ; Triebl, Alexander ; Trötzmüller, Martin ; Ubhayasekera, S. J.Kumari ; Vuckovic, Dajana ; Weir, Jacquelyn M. ; Welti, Ruth ; Wenk, Markus R. ; Wheelock, Craig E. ; Yao, Libin ; Yuan, Min ; Zhao, Xueqing Heather ; Zhou, Senlin. / Harmonizing lipidomics : NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in frozen human plasma. In: Journal of Lipid Research. 2017 ; Vol. 58, No. 12. pp. 2275-2288.
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T1 - Harmonizing lipidomics

T2 - NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in frozen human plasma

AU - Bowden, John A.

AU - Heckert, Alan

AU - Ulmer, Candice Z.

AU - Jones, Christina M.

AU - Koelmel, Jeremy P.

AU - Abdullah, Laila

AU - Ahonen, Linda

AU - Alnouti, Yazen

AU - Armando, Aaron M.

AU - Asara, John M.

AU - Bamba, Takeshi

AU - Barr, John R.

AU - Bergquist, Jonas

AU - Borchers, Christoph H.

AU - Brandsma, Joost

AU - Breitkopf, Susanne B.

AU - Cajka, Tomas

AU - Cazenave-Gassiot, Amaury

AU - Checa, Antonio

AU - Cinel, Michelle A.

AU - Colas, Romain A.

AU - Cremers, Serge

AU - Dennis, Edward A.

AU - Evans, James E.

AU - Fauland, Alexander

AU - Fiehn, Oliver

AU - Gardner, Michael S.

AU - Garrett, Timothy J.

AU - Gotlinger, Katherine H.

AU - Han, Jun

AU - Huang, Yingying

AU - Neo, Aveline Huipeng

AU - Hyötyläinen, Tuulia

AU - Izumi, Yoshihiro

AU - Jiang, Hongfeng

AU - Jiang, Houli

AU - Jiang, Jiang

AU - Kachman, Maureen

AU - Kiyonami, Reiko

AU - Klavins, Kristaps

AU - Klose, Christian

AU - Köfeler, Harald C.

AU - Kolmert, Johan

AU - Koal, Therese

AU - Koster, Grielof

AU - Kuklenyik, Zsuzsanna

AU - Kurland, Irwin J.

AU - Leadley, Michael

AU - Lin, Karen

AU - Maddipati, Krishna Rao

AU - McDougall, Danielle

AU - Meikle, Peter J.

AU - Mellett, Natalie A.

AU - Monnin, Cian

AU - Moseley, M. Arthur

AU - Nandakumar, Renu

AU - Oresic, Matej

AU - Patterson, Rainey

AU - Peake, David

AU - Pierce, Jason S.

AU - Post, Martin

AU - Postle, Anthony D.

AU - Pugh, Rebecca

AU - Qiu, Yunping

AU - Quehenberger, Oswald

AU - Ramrup, Parsram

AU - Rees, Jon

AU - Rembiesa, Barbara

AU - Reynaud, Denis

AU - Roth, Mary R.

AU - Sales, Susanne

AU - Schuhmann, Kai

AU - Schwartzman, Michal Laniado

AU - Serhan, Charles N.

AU - Shevchenko, Andrej

AU - Somerville, Stephen E.

AU - St John-Williams, Lisa

AU - Surma, Michal A.

AU - Takeda, Hiroaki

AU - Thakare, Rhishikesh

AU - Thompson, J. Will

AU - Torta, Federico

AU - Triebl, Alexander

AU - Trötzmüller, Martin

AU - Ubhayasekera, S. J.Kumari

AU - Vuckovic, Dajana

AU - Weir, Jacquelyn M.

AU - Welti, Ruth

AU - Wenk, Markus R.

AU - Wheelock, Craig E.

AU - Yao, Libin

AU - Yuan, Min

AU - Zhao, Xueqing Heather

AU - Zhou, Senlin

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KW - Glycerolipids

KW - Lipids

KW - National institute of standards and technology

KW - Phospholipids

KW - Quality control

KW - Quantitation

KW - Sphingolipids

KW - Standard reference material 1950

KW - Sterols

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