Halo-free Phase Contrast Microscopy

Tan H. Nguyen, Mikhail Kandel, Haadi M. Shakir, Catherine Best-Popescu, Jyothi Arikkath, Minh N. Do, Gabriel Popescu

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Abstract

We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Acquiring four independent intensity images, our approach first measures haloed phase maps of the sample. We solve for the halo-free sample transmission function by using a physical model of the image formation under partial spatial coherence. Using this halo-free sample transmission, we can numerically generate artifact-free PC images. Furthermore, this transmission can be further used to obtain quantitative information about the sample, e.g., the thickness with known refractive indices, dry mass of live cells during their cycles. We tested our hfPC method on various control samples, e.g., beads, pillars and validated its potential for biological investigation by imaging live HeLa cells, red blood cells, and neurons.

Original languageEnglish (US)
Article number44034
JournalScientific reports
Volume7
DOIs
Publication statusPublished - Mar 24 2017

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Nguyen, T. H., Kandel, M., Shakir, H. M., Best-Popescu, C., Arikkath, J., Do, M. N., & Popescu, G. (2017). Halo-free Phase Contrast Microscopy. Scientific reports, 7, [44034]. https://doi.org/10.1038/srep44034