Halo-free Phase Contrast Microscopy

Tan H. Nguyen, Mikhail Kandel, Haadi M. Shakir, Catherine Best-Popescu, Jyothi Arikkath, Minh N. Do, Gabriel Popescu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Acquiring four independent intensity images, our approach first measures haloed phase maps of the sample. We solve for the halo-free sample transmission function by using a physical model of the image formation under partial spatial coherence. Using this halo-free sample transmission, we can numerically generate artifact-free PC images. Furthermore, this transmission can be further used to obtain quantitative information about the sample, e.g., the thickness with known refractive indices, dry mass of live cells during their cycles. We tested our hfPC method on various control samples, e.g., beads, pillars and validated its potential for biological investigation by imaging live HeLa cells, red blood cells, and neurons.

Original languageEnglish (US)
Article number44034
JournalScientific reports
Volume7
DOIs
StatePublished - Mar 24 2017

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phase contrast
halos
microscopy
artifacts
upgrading
reversing
erythrocytes
cells
neurons
beads
modules
microscopes
refractivity
cycles

ASJC Scopus subject areas

  • General

Cite this

Nguyen, T. H., Kandel, M., Shakir, H. M., Best-Popescu, C., Arikkath, J., Do, M. N., & Popescu, G. (2017). Halo-free Phase Contrast Microscopy. Scientific reports, 7, [44034]. https://doi.org/10.1038/srep44034

Halo-free Phase Contrast Microscopy. / Nguyen, Tan H.; Kandel, Mikhail; Shakir, Haadi M.; Best-Popescu, Catherine; Arikkath, Jyothi; Do, Minh N.; Popescu, Gabriel.

In: Scientific reports, Vol. 7, 44034, 24.03.2017.

Research output: Contribution to journalArticle

Nguyen, TH, Kandel, M, Shakir, HM, Best-Popescu, C, Arikkath, J, Do, MN & Popescu, G 2017, 'Halo-free Phase Contrast Microscopy', Scientific reports, vol. 7, 44034. https://doi.org/10.1038/srep44034
Nguyen TH, Kandel M, Shakir HM, Best-Popescu C, Arikkath J, Do MN et al. Halo-free Phase Contrast Microscopy. Scientific reports. 2017 Mar 24;7. 44034. https://doi.org/10.1038/srep44034
Nguyen, Tan H. ; Kandel, Mikhail ; Shakir, Haadi M. ; Best-Popescu, Catherine ; Arikkath, Jyothi ; Do, Minh N. ; Popescu, Gabriel. / Halo-free Phase Contrast Microscopy. In: Scientific reports. 2017 ; Vol. 7.
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