Half-life of chlorpyrifos oxon and other organophosphorus esters in aqueous solution

Research output: Contribution to journalArticle

Abstract

Aqueous solutions of chlorpyrifos oxon are used to study the ability of chlorpyrifos oxon to catalyze protein crosslinking. Assays for protein crosslinking can avoid artifacts by using information on the stability of chlorpyrifos oxon in solution. We undertook to determine the half-life of chlorpyrifos oxon in aqueous solution because literature values do not exist. The rate of conversion of chlorpyrifos oxon to 3,5,6-trichloro-2-pyridinol was measured at 23 °C in 20 mM TrisCl pH 8 and pH 9 by recording loss of absorbance at 290 nm for chlorpyrifos oxon and increase in absorbance at 320 nm for 3,5,6-trichloro-2-pyridinol. The half-life of chlorpyrifos oxon was 20.9 days at pH 8 and 6.7 days at pH 9. Literature reports for the stability of other organophosphorus toxicants were summarized because our current studies suggest that other organophosphorus toxicants are also crosslinking agents.

Original languageEnglish (US)
Article number108788
JournalChemico-Biological Interactions
Volume311
DOIs
StatePublished - Sep 25 2019

Fingerprint

Half-Life
Esters
Crosslinking
O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate
Artifacts
Assays
Proteins

Keywords

  • Absorbance spectra
  • Chlorpyrifos oxon
  • Extinction coefficient
  • Half-life
  • Stability

ASJC Scopus subject areas

  • Toxicology

Cite this

Half-life of chlorpyrifos oxon and other organophosphorus esters in aqueous solution. / Lockridge, Oksana; Verdier, Laurent; Schopfer, Lawrence M.

In: Chemico-Biological Interactions, Vol. 311, 108788, 25.09.2019.

Research output: Contribution to journalArticle

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