HAESA, an Arabidopsis leucine-rich repeat receptor kinase, controls floral organ abscission

Tsung Luo Jinn, Julie M Stone, John C. Walker

Research output: Contribution to journalArticle

251 Citations (Scopus)

Abstract

Abcission, the natural shedding of leaves, flowers and fruits, is a fundamental component of plant development. Abscission is a highly regulated process that occurs at distinct zones of cells that undergo enlargement and subsequent separation. Although some components of abscission, including accumulation of the hormone ethylene and cell wall-degrading enzymes, have been described, the regulatory pathways remain largely unknown. In this paper we described a critical component required for floral organ abscission in Arabidopsis thaliana, the receptor-like protein kinase HAESA. Histochemical analysis of transgenic plants harboring a HAESA promoter:: β-glucuronidase reporter gene and in situ RNA hybridization experiments show HAESA expression in the abscission zones where the sepals, petals, and stamens attach to the receptacle, at the base of pedicels, and at the base of petioles where leaves attach to the stem Immunodetection, immunoprecipitation, and protein kinase activity assays reveal HAESA is a plasma membrane serine/threonine protein kinase. The reduction of function of HAESA in transgenic plants harboring an antisense construct results in delayed abscission of floral organs, and the severity of the phenotype is directly correlated with the level of HAESA protein. These results demonstrate that HAESA functions in developmentally regulated floral organ abscission.

Original languageEnglish (US)
Pages (from-to)108-117
Number of pages10
JournalGenes and Development
Volume14
Issue number1
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Genetically Modified Plants
Arabidopsis
Leucine
Protein Kinases
Phosphotransferases
Cell Enlargement
Plant Development
Protein-Serine-Threonine Kinases
Glucuronidase
Reporter Genes
Immunoprecipitation
Cell Wall
In Situ Hybridization
Fruit
Cell Membrane
Hormones
RNA
Phenotype
Enzymes
Proteins

Keywords

  • Abscission
  • Arabidopsis
  • HAE
  • HAESA
  • RLK
  • Receptor kinase

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

HAESA, an Arabidopsis leucine-rich repeat receptor kinase, controls floral organ abscission. / Jinn, Tsung Luo; Stone, Julie M; Walker, John C.

In: Genes and Development, Vol. 14, No. 1, 01.01.2000, p. 108-117.

Research output: Contribution to journalArticle

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