Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5 −/− mice

Yan Li, Roberto Tinoco, Lisa Elmén, Igor Segota, Yibo Xian, Yu Fujita, Avinash Sahu, Raphy Zarecki, Kerrie Marie, Yongmei Feng, Ali Khateb, Dennie T. Frederick, Shiri K. Ashkenazi, Hyungsoo Kim, Eva Guijarro Perez, Chi Ping Day, Rafael S. Segura Muñoz, Robert Schmaltz, Shibu Yooseph, Miguel A. TamTongwu Zhang, Emily Avitan-Hersh, Lihi Tzur, Shoshana Roizman, Ilanit Boyango, Gil Bar-Sela, Amir Orian, Randal J. Kaufman, Marcus Bosenberg, Colin R. Goding, Bas Baaten, Mitchell P. Levesque, Reinhard Dummer, Kevin Brown, Glenn Merlino, Eytan Ruppin, Keith Flaherty, Amanda Ramer-Tait, Tao Long, Scott N. Peterson, Linda M. Bradley, Ze’ev A. Ronai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Accumulating evidence points to an important role for the gut microbiome in anti-tumor immunity. Here, we show that altered intestinal microbiota contributes to anti-tumor immunity, limiting tumor expansion. Mice lacking the ubiquitin ligase RNF5 exhibit attenuated activation of the unfolded protein response (UPR) components, which coincides with increased expression of inflammasome components, recruitment and activation of dendritic cells and reduced expression of antimicrobial peptides in intestinal epithelial cells. Reduced UPR expression is also seen in murine and human melanoma tumor specimens that responded to immune checkpoint therapy. Co-housing of Rnf5 −/− and WT mice abolishes the anti-tumor immunity and tumor inhibition phenotype, whereas transfer of 11 bacterial strains, including B. rodentium, enriched in Rnf5 −/− mice, establishes anti-tumor immunity and restricts melanoma growth in germ-free WT mice. Altered UPR signaling, exemplified in Rnf5 −/− mice, coincides with altered gut microbiota composition and anti-tumor immunity to control melanoma growth.

Original languageEnglish (US)
Article number1492
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

immunity
mice
Tumors
Immunity
Melanoma
tumors
Growth
Unfolded Protein Response
Neoplasms
proteins
Chemical activation
activation
Inflammasomes
Proteins
phenotype
Gastrointestinal Microbiome
Ligases
Ubiquitin
Dendritic Cells
peptides

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Li, Y., Tinoco, R., Elmén, L., Segota, I., Xian, Y., Fujita, Y., ... Ronai, Z. A. (2019). Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5 −/− mice Nature communications, 10(1), [1492]. https://doi.org/10.1038/s41467-019-09525-y

Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5 −/− mice . / Li, Yan; Tinoco, Roberto; Elmén, Lisa; Segota, Igor; Xian, Yibo; Fujita, Yu; Sahu, Avinash; Zarecki, Raphy; Marie, Kerrie; Feng, Yongmei; Khateb, Ali; Frederick, Dennie T.; Ashkenazi, Shiri K.; Kim, Hyungsoo; Perez, Eva Guijarro; Day, Chi Ping; Segura Muñoz, Rafael S.; Schmaltz, Robert; Yooseph, Shibu; Tam, Miguel A.; Zhang, Tongwu; Avitan-Hersh, Emily; Tzur, Lihi; Roizman, Shoshana; Boyango, Ilanit; Bar-Sela, Gil; Orian, Amir; Kaufman, Randal J.; Bosenberg, Marcus; Goding, Colin R.; Baaten, Bas; Levesque, Mitchell P.; Dummer, Reinhard; Brown, Kevin; Merlino, Glenn; Ruppin, Eytan; Flaherty, Keith; Ramer-Tait, Amanda; Long, Tao; Peterson, Scott N.; Bradley, Linda M.; Ronai, Ze’ev A.

In: Nature communications, Vol. 10, No. 1, 1492, 01.12.2019.

Research output: Contribution to journalArticle

Li, Y, Tinoco, R, Elmén, L, Segota, I, Xian, Y, Fujita, Y, Sahu, A, Zarecki, R, Marie, K, Feng, Y, Khateb, A, Frederick, DT, Ashkenazi, SK, Kim, H, Perez, EG, Day, CP, Segura Muñoz, RS, Schmaltz, R, Yooseph, S, Tam, MA, Zhang, T, Avitan-Hersh, E, Tzur, L, Roizman, S, Boyango, I, Bar-Sela, G, Orian, A, Kaufman, RJ, Bosenberg, M, Goding, CR, Baaten, B, Levesque, MP, Dummer, R, Brown, K, Merlino, G, Ruppin, E, Flaherty, K, Ramer-Tait, A, Long, T, Peterson, SN, Bradley, LM & Ronai, ZA 2019, ' Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5 −/− mice ', Nature communications, vol. 10, no. 1, 1492. https://doi.org/10.1038/s41467-019-09525-y
Li, Yan ; Tinoco, Roberto ; Elmén, Lisa ; Segota, Igor ; Xian, Yibo ; Fujita, Yu ; Sahu, Avinash ; Zarecki, Raphy ; Marie, Kerrie ; Feng, Yongmei ; Khateb, Ali ; Frederick, Dennie T. ; Ashkenazi, Shiri K. ; Kim, Hyungsoo ; Perez, Eva Guijarro ; Day, Chi Ping ; Segura Muñoz, Rafael S. ; Schmaltz, Robert ; Yooseph, Shibu ; Tam, Miguel A. ; Zhang, Tongwu ; Avitan-Hersh, Emily ; Tzur, Lihi ; Roizman, Shoshana ; Boyango, Ilanit ; Bar-Sela, Gil ; Orian, Amir ; Kaufman, Randal J. ; Bosenberg, Marcus ; Goding, Colin R. ; Baaten, Bas ; Levesque, Mitchell P. ; Dummer, Reinhard ; Brown, Kevin ; Merlino, Glenn ; Ruppin, Eytan ; Flaherty, Keith ; Ramer-Tait, Amanda ; Long, Tao ; Peterson, Scott N. ; Bradley, Linda M. ; Ronai, Ze’ev A. / Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5 −/− mice In: Nature communications. 2019 ; Vol. 10, No. 1.
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AU - Sahu, Avinash

AU - Zarecki, Raphy

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AU - Feng, Yongmei

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AU - Yooseph, Shibu

AU - Tam, Miguel A.

AU - Zhang, Tongwu

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AU - Levesque, Mitchell P.

AU - Dummer, Reinhard

AU - Brown, Kevin

AU - Merlino, Glenn

AU - Ruppin, Eytan

AU - Flaherty, Keith

AU - Ramer-Tait, Amanda

AU - Long, Tao

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