Guiding Mesenchymal Stem Cells into Myelinating Schwann Cell-Like Phenotypes by Using Electrospun Core-Sheath Nanoyarns

Shaohua Wu, Shilei Ni, Xiping Jiang, Mitchell A. Kuss, Han Jun Wang, Bin Duan

Research output: Contribution to journalArticle

Abstract

Nerve guidance conduit (NGC)-infilling substrates have been reported to facilitate the regeneration of injured peripheral nerves (PNs), especially for large nerve gaps. In this study, longitudinally oriented electrospun core-sheath nanoyarns (csNYs), consisting of a polylactic acid microfiber core and an electrospun nanofiber sheath, were fabricated for potential PN tissue engineering applications. Our novel csNY displayed a well-aligned nanofibrous surface topography, resembling the ultrastructure of axons and fascicles of a native PN system, and it also provided a mechanically stable structure. The biological results showed that the csNY significantly enhanced the attachment, growth, and proliferation of human adipose derived mesenchymal stem cells (hADMSC) and also promoted the migration, proliferation, and phenotype maintenance of rabbit Schwann cells (rSCs). Our csNY notably increased the differentiation capability of hADMSC into SC-like cells (hADMSC-SC), in comparison with a 2D tissue culture polystyrene plate. More importantly, when combined with the appropriate induction medium, our csNY promoted hADMSC-SC to express high levels of myelination-associated markers. Overall, this study demonstrates that our csNYs have great potential to serve as not only ideal in vitro culture models for understanding SC-axon interaction and SC myelination but also as promising NGC-infilling substrates for PN regeneration applications.

Original languageEnglish (US)
Pages (from-to)5284-5294
Number of pages11
JournalACS Biomaterials Science and Engineering
Volume5
Issue number10
DOIs
StatePublished - Oct 14 2019

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Stem cells
Cells
Tissue culture
Polystyrenes
Bioelectric potentials
Surface topography
Substrates
Nanofibers
Tissue engineering
Acids
Axons

Keywords

  • Schwann cell-like differentiation
  • cell migration
  • core-sheath yarn
  • myelination
  • nerve regeneration

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Guiding Mesenchymal Stem Cells into Myelinating Schwann Cell-Like Phenotypes by Using Electrospun Core-Sheath Nanoyarns. / Wu, Shaohua; Ni, Shilei; Jiang, Xiping; Kuss, Mitchell A.; Wang, Han Jun; Duan, Bin.

In: ACS Biomaterials Science and Engineering, Vol. 5, No. 10, 14.10.2019, p. 5284-5294.

Research output: Contribution to journalArticle

Wu, Shaohua ; Ni, Shilei ; Jiang, Xiping ; Kuss, Mitchell A. ; Wang, Han Jun ; Duan, Bin. / Guiding Mesenchymal Stem Cells into Myelinating Schwann Cell-Like Phenotypes by Using Electrospun Core-Sheath Nanoyarns. In: ACS Biomaterials Science and Engineering. 2019 ; Vol. 5, No. 10. pp. 5284-5294.
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