GSH protects against oxidative stress and toxicity in VL-17A cells exposed to high glucose

S. Mathan Kumar, Kavitha Swaminathan, Dahn L Clemens, Aparajita Dey

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: The deficiency of glutathione (GSH) has been linked to several diseases. The study investigated the role of GSH as a protective factor against hyperglycemia-mediated injury in VL-17A cells treated with 50 mM glucose. Methods: The cell viability and different oxidative stress parameters including glyoxalase I activity were measured. Results: GSH supplementation with 2 mM N-acetyl cysteine (NAC) or 0.1 mM ursodeoxycholic acid (UDCA) increased the viability, GSH level and the GSH-dependent glyoxalase I activity in 50 mM glucose-treated VL-17A cells. Further, pretreatment of 50 mM glucose-treated VL-17A cells with NAC or UDCA decreased oxidative stress (levels of reactive oxygen species and protein carbonylation), apoptosis (caspase 3 activity and annexin V–propidium iodide positive cells) and glutathionylated protein formation, a measure of oxidative stress. GSH depletion with 0.4 mM buthionine sulfoximine (BSO) or 1 mM diethyl maleate (DEM) potentiated the decrease in viability, glyoxalase I activity and increase in oxidative stress and apoptosis, with decreased GSH levels in 50 mM glucose-treated VL-17A cells. Conclusion: Thus, changes in GSH levels with exogenous agents such as NAC, UDCA, BSO or DEM modulate hyperglycemia-mediated injury in a cell model of VL-17A liver cells.

Original languageEnglish (US)
Pages (from-to)223-234
Number of pages12
JournalEuropean Journal of Nutrition
Volume54
Issue number2
DOIs
StatePublished - Jan 1 2015

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Oxidative Stress
diethyl maleate
Glucose
Lactoylglutathione Lyase
Ursodeoxycholic Acid
Buthionine Sulfoximine
Cysteine
Hyperglycemia
Protein Carbonylation
Apoptosis
Annexins
Wounds and Injuries
Iodides
Caspase 3
Glutathione
Reactive Oxygen Species
Cell Survival
Liver
Proteins

Keywords

  • Glutathione
  • Hyperglycemia
  • Injury
  • Liver
  • Reactive oxygen species

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

GSH protects against oxidative stress and toxicity in VL-17A cells exposed to high glucose. / Kumar, S. Mathan; Swaminathan, Kavitha; Clemens, Dahn L; Dey, Aparajita.

In: European Journal of Nutrition, Vol. 54, No. 2, 01.01.2015, p. 223-234.

Research output: Contribution to journalArticle

Kumar, S. Mathan ; Swaminathan, Kavitha ; Clemens, Dahn L ; Dey, Aparajita. / GSH protects against oxidative stress and toxicity in VL-17A cells exposed to high glucose. In: European Journal of Nutrition. 2015 ; Vol. 54, No. 2. pp. 223-234.
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