Growth/cell cycle regulation of Sp1 phosphorylation

Adrian R. Black, David Jensen, Shiaw Yih Lin, Jane Clifford Azizkhan

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Sp1 sites can mediate growth/cell cycle induction of dihydrofolate reductase in late G1 (Jensen, D. E., Black, A. R. Swick, A. G., and Azizkhan, J. C. (1997) J. Cell. Biochem. 67, 24-31). To investigate mechanisms underlying this induction, effects of serum stimulation on regulation of Sp1 were examined. In Balb/c 3T3 cells, serum stimulation did not affect Sp1 synthesis or the relative binding of Sp1 family members to DNA; however, it did result in a rapid, ~-fold increase in Sp1 levels and an ~3-fold increase in specific Sp1 phosphorylation in midG1. In normal human diploid fibroblasts, serum stimulation also increased Sp1 phosphorylation in mid-G1 but did not affect Sp1 levels. Therefore, Sp1 phosphorylation is regulated in a growth/cell cycle-dependent manner which correlates temporally with induction of dihydrofolate reductase transcription. Further studies revealed a kinase activity specifically associated with Sp1 in a growth- regulated manner. This activity is distinct from purified kinases previously shown to phosphorylate Sp1 in vitro and phosphorylates Sp1 between amino acids 612 and 678 in its C terminus, a region also phosphorylated in mid-G1 in vivo. Therefore, this study indicates that phosphorylation of the C terminus of Sp1 may play a role in the cell cycle regulation of its transcriptional activity.

Original languageEnglish (US)
Pages (from-to)1207-1215
Number of pages9
JournalJournal of Biological Chemistry
Volume274
Issue number3
DOIs
StatePublished - Jan 15 1999

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Phosphorylation
Cell growth
Cell Cycle
Tetrahydrofolate Dehydrogenase
Growth
Phosphotransferases
Serum
3T3 Cells
Transcription
Fibroblasts
Diploidy
Cells
Amino Acids
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Growth/cell cycle regulation of Sp1 phosphorylation. / Black, Adrian R.; Jensen, David; Lin, Shiaw Yih; Azizkhan, Jane Clifford.

In: Journal of Biological Chemistry, Vol. 274, No. 3, 15.01.1999, p. 1207-1215.

Research output: Contribution to journalArticle

Black, Adrian R. ; Jensen, David ; Lin, Shiaw Yih ; Azizkhan, Jane Clifford. / Growth/cell cycle regulation of Sp1 phosphorylation. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 3. pp. 1207-1215.
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