Growth stimulation by transfection of intestinal epithelial cells with an antisense insulin-like growth factor-binding protein-2 construct

M. R. Corkins, J. A. Vanderhoof, D. H. Slentz, Richard G MacDonald, J. H Y Park

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

IEC-6 cells, an intestinal epithelial cell line, produce insulin-like growth factor (IGF)-II and IGF-binding protein-2 (IGFBP-2). Exogenous IGFs stimulate and IGFBP-2 attenuates DNA synthesis. To investigate whether endogenously secreted IGFBP-2 inhibits proliferation, IEC-6 cells were transfected with a full-length rat IGFBP-2 cDNA antisense expression construct. The steady-state level of IGFBP-2 mRNA decreased by 54% in the antisense cDNA-transfected cells (denoted revBP2) compared with vector-transfected controls. Moreover, revBP2 cells secreted less IGFBP-2 than controls (maximally a 68% decrease). In serum-containing medium, revBP2 cells exhibited a 35% increase in log-phase proliferation rate and growth-arrested at a maximum density 14% higher than controls. We conclude that endogenous IGFBP-2 inhibits proliferation of IEC-6 cells, probably by sequestering IGFs.

Original languageEnglish (US)
Pages (from-to)707-713
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume211
Issue number3
DOIs
StatePublished - Jun 26 1995

Fingerprint

Insulin-Like Growth Factor Binding Protein 2
Transfection
Epithelial Cells
Growth
Complementary DNA
Insulin-Like Growth Factor II
Rats
Cell Line
Messenger RNA
DNA
Serum

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Growth stimulation by transfection of intestinal epithelial cells with an antisense insulin-like growth factor-binding protein-2 construct. / Corkins, M. R.; Vanderhoof, J. A.; Slentz, D. H.; MacDonald, Richard G; Park, J. H Y.

In: Biochemical and Biophysical Research Communications, Vol. 211, No. 3, 26.06.1995, p. 707-713.

Research output: Contribution to journalArticle

@article{212b25db01ab4a8e9d66f2b43adffb9c,
title = "Growth stimulation by transfection of intestinal epithelial cells with an antisense insulin-like growth factor-binding protein-2 construct",
abstract = "IEC-6 cells, an intestinal epithelial cell line, produce insulin-like growth factor (IGF)-II and IGF-binding protein-2 (IGFBP-2). Exogenous IGFs stimulate and IGFBP-2 attenuates DNA synthesis. To investigate whether endogenously secreted IGFBP-2 inhibits proliferation, IEC-6 cells were transfected with a full-length rat IGFBP-2 cDNA antisense expression construct. The steady-state level of IGFBP-2 mRNA decreased by 54{\%} in the antisense cDNA-transfected cells (denoted revBP2) compared with vector-transfected controls. Moreover, revBP2 cells secreted less IGFBP-2 than controls (maximally a 68{\%} decrease). In serum-containing medium, revBP2 cells exhibited a 35{\%} increase in log-phase proliferation rate and growth-arrested at a maximum density 14{\%} higher than controls. We conclude that endogenous IGFBP-2 inhibits proliferation of IEC-6 cells, probably by sequestering IGFs.",
author = "Corkins, {M. R.} and Vanderhoof, {J. A.} and Slentz, {D. H.} and MacDonald, {Richard G} and Park, {J. H Y}",
year = "1995",
month = "6",
day = "26",
doi = "10.1006/bbrc.1995.1870",
language = "English (US)",
volume = "211",
pages = "707--713",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Growth stimulation by transfection of intestinal epithelial cells with an antisense insulin-like growth factor-binding protein-2 construct

AU - Corkins, M. R.

AU - Vanderhoof, J. A.

AU - Slentz, D. H.

AU - MacDonald, Richard G

AU - Park, J. H Y

PY - 1995/6/26

Y1 - 1995/6/26

N2 - IEC-6 cells, an intestinal epithelial cell line, produce insulin-like growth factor (IGF)-II and IGF-binding protein-2 (IGFBP-2). Exogenous IGFs stimulate and IGFBP-2 attenuates DNA synthesis. To investigate whether endogenously secreted IGFBP-2 inhibits proliferation, IEC-6 cells were transfected with a full-length rat IGFBP-2 cDNA antisense expression construct. The steady-state level of IGFBP-2 mRNA decreased by 54% in the antisense cDNA-transfected cells (denoted revBP2) compared with vector-transfected controls. Moreover, revBP2 cells secreted less IGFBP-2 than controls (maximally a 68% decrease). In serum-containing medium, revBP2 cells exhibited a 35% increase in log-phase proliferation rate and growth-arrested at a maximum density 14% higher than controls. We conclude that endogenous IGFBP-2 inhibits proliferation of IEC-6 cells, probably by sequestering IGFs.

AB - IEC-6 cells, an intestinal epithelial cell line, produce insulin-like growth factor (IGF)-II and IGF-binding protein-2 (IGFBP-2). Exogenous IGFs stimulate and IGFBP-2 attenuates DNA synthesis. To investigate whether endogenously secreted IGFBP-2 inhibits proliferation, IEC-6 cells were transfected with a full-length rat IGFBP-2 cDNA antisense expression construct. The steady-state level of IGFBP-2 mRNA decreased by 54% in the antisense cDNA-transfected cells (denoted revBP2) compared with vector-transfected controls. Moreover, revBP2 cells secreted less IGFBP-2 than controls (maximally a 68% decrease). In serum-containing medium, revBP2 cells exhibited a 35% increase in log-phase proliferation rate and growth-arrested at a maximum density 14% higher than controls. We conclude that endogenous IGFBP-2 inhibits proliferation of IEC-6 cells, probably by sequestering IGFs.

UR - http://www.scopus.com/inward/record.url?scp=0029017697&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029017697&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1995.1870

DO - 10.1006/bbrc.1995.1870

M3 - Article

VL - 211

SP - 707

EP - 713

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -