Growth inhibition and differentiation of the human colon carcinoma cell line, Caco-2, by constitutive expression of insulin-like growth factor binding protein-3

Richard G MacDonald, Beverly S. Schaffer, Il Jun Kang, Soon M. Hong, Eun J. Kim, Jung H Y Park

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The human colon carcinoma cell line, Caco-2, produces insulin-like growth factor binding protein-3 (IGFBP-3), the secretion of which correlates with markers of enterocyte differentiation. To investigate whether IGFBP-3 inhibits proliferation or induces differentiation, Caco-2 cells were stably transfected with an IGFBP-3 cDNA expression construct or pcDNA3 vector as a control. Accumulation of IGFBP-3 mRNA and secretion of the protein into conditioned medium 9 days after plating were readily detected in the transfected cells, whereas these parameters were undetectable in pcDNA3- transfected cells. Insulin-like growth factor binding protein-3-expressing cells grew at a rate similar to the controls for 6 days after plating, but achieved a much lower final density between days 10 and 12. By day 9 of culture, accumulation of sucrase-isomaltase mRNA, a marker of enterocytic differentiation of Caco-2 cells, was evident in the IGFBP-3-expressing cells, but was undetectable in the controls. These results indicate that IGFBP-3 may inhibit proliferation and induce early differentiation of Caco-2 cells.

Original languageEnglish (US)
Pages (from-to)72-78
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume14
Issue number1
DOIs
StatePublished - Jan 1999

Fingerprint

Insulin-Like Growth Factor Binding Protein 3
Colon
Carcinoma
Cell Line
Growth
Caco-2 Cells
Differentiation Antigens
Oligo-1,6-Glucosidase
Sucrase
Messenger RNA
Enterocytes
Conditioned Culture Medium
Complementary DNA

Keywords

  • Caco-2
  • Colon carcinoma
  • Differentiation
  • Insulin-like growth factor binding protein-3
  • Insulin-like growth factors
  • Markers of intestinal differentiation
  • Proliferation

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Growth inhibition and differentiation of the human colon carcinoma cell line, Caco-2, by constitutive expression of insulin-like growth factor binding protein-3. / MacDonald, Richard G; Schaffer, Beverly S.; Kang, Il Jun; Hong, Soon M.; Kim, Eun J.; Park, Jung H Y.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 14, No. 1, 01.1999, p. 72-78.

Research output: Contribution to journalArticle

@article{5f8578fb8bae49d7b90d40b117ac7b60,
title = "Growth inhibition and differentiation of the human colon carcinoma cell line, Caco-2, by constitutive expression of insulin-like growth factor binding protein-3",
abstract = "The human colon carcinoma cell line, Caco-2, produces insulin-like growth factor binding protein-3 (IGFBP-3), the secretion of which correlates with markers of enterocyte differentiation. To investigate whether IGFBP-3 inhibits proliferation or induces differentiation, Caco-2 cells were stably transfected with an IGFBP-3 cDNA expression construct or pcDNA3 vector as a control. Accumulation of IGFBP-3 mRNA and secretion of the protein into conditioned medium 9 days after plating were readily detected in the transfected cells, whereas these parameters were undetectable in pcDNA3- transfected cells. Insulin-like growth factor binding protein-3-expressing cells grew at a rate similar to the controls for 6 days after plating, but achieved a much lower final density between days 10 and 12. By day 9 of culture, accumulation of sucrase-isomaltase mRNA, a marker of enterocytic differentiation of Caco-2 cells, was evident in the IGFBP-3-expressing cells, but was undetectable in the controls. These results indicate that IGFBP-3 may inhibit proliferation and induce early differentiation of Caco-2 cells.",
keywords = "Caco-2, Colon carcinoma, Differentiation, Insulin-like growth factor binding protein-3, Insulin-like growth factors, Markers of intestinal differentiation, Proliferation",
author = "MacDonald, {Richard G} and Schaffer, {Beverly S.} and Kang, {Il Jun} and Hong, {Soon M.} and Kim, {Eun J.} and Park, {Jung H Y}",
year = "1999",
month = "1",
doi = "10.1046/j.1440-1746.1999.01803.x",
language = "English (US)",
volume = "14",
pages = "72--78",
journal = "Journal of Gastroenterology and Hepatology (Australia)",
issn = "0815-9319",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Growth inhibition and differentiation of the human colon carcinoma cell line, Caco-2, by constitutive expression of insulin-like growth factor binding protein-3

AU - MacDonald, Richard G

AU - Schaffer, Beverly S.

AU - Kang, Il Jun

AU - Hong, Soon M.

AU - Kim, Eun J.

AU - Park, Jung H Y

PY - 1999/1

Y1 - 1999/1

N2 - The human colon carcinoma cell line, Caco-2, produces insulin-like growth factor binding protein-3 (IGFBP-3), the secretion of which correlates with markers of enterocyte differentiation. To investigate whether IGFBP-3 inhibits proliferation or induces differentiation, Caco-2 cells were stably transfected with an IGFBP-3 cDNA expression construct or pcDNA3 vector as a control. Accumulation of IGFBP-3 mRNA and secretion of the protein into conditioned medium 9 days after plating were readily detected in the transfected cells, whereas these parameters were undetectable in pcDNA3- transfected cells. Insulin-like growth factor binding protein-3-expressing cells grew at a rate similar to the controls for 6 days after plating, but achieved a much lower final density between days 10 and 12. By day 9 of culture, accumulation of sucrase-isomaltase mRNA, a marker of enterocytic differentiation of Caco-2 cells, was evident in the IGFBP-3-expressing cells, but was undetectable in the controls. These results indicate that IGFBP-3 may inhibit proliferation and induce early differentiation of Caco-2 cells.

AB - The human colon carcinoma cell line, Caco-2, produces insulin-like growth factor binding protein-3 (IGFBP-3), the secretion of which correlates with markers of enterocyte differentiation. To investigate whether IGFBP-3 inhibits proliferation or induces differentiation, Caco-2 cells were stably transfected with an IGFBP-3 cDNA expression construct or pcDNA3 vector as a control. Accumulation of IGFBP-3 mRNA and secretion of the protein into conditioned medium 9 days after plating were readily detected in the transfected cells, whereas these parameters were undetectable in pcDNA3- transfected cells. Insulin-like growth factor binding protein-3-expressing cells grew at a rate similar to the controls for 6 days after plating, but achieved a much lower final density between days 10 and 12. By day 9 of culture, accumulation of sucrase-isomaltase mRNA, a marker of enterocytic differentiation of Caco-2 cells, was evident in the IGFBP-3-expressing cells, but was undetectable in the controls. These results indicate that IGFBP-3 may inhibit proliferation and induce early differentiation of Caco-2 cells.

KW - Caco-2

KW - Colon carcinoma

KW - Differentiation

KW - Insulin-like growth factor binding protein-3

KW - Insulin-like growth factors

KW - Markers of intestinal differentiation

KW - Proliferation

UR - http://www.scopus.com/inward/record.url?scp=0033020510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033020510&partnerID=8YFLogxK

U2 - 10.1046/j.1440-1746.1999.01803.x

DO - 10.1046/j.1440-1746.1999.01803.x

M3 - Article

C2 - 10029281

AN - SCOPUS:0033020510

VL - 14

SP - 72

EP - 78

JO - Journal of Gastroenterology and Hepatology (Australia)

JF - Journal of Gastroenterology and Hepatology (Australia)

SN - 0815-9319

IS - 1

ER -