Growth hormone and lactogenic hormones can reduce the leptin mRNA expression in bovine mammary epithelial cells

Shinichi Yonekura, Kazuhito Sakamoto, Tokushi Komatsu, Akihiko Hagino, Kazuo Katoh, Yoshiaki Obara

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Leptin mRNA is expressed in not only adipocytes but also mammary epithelial cells and leptin protein is present in milk. Although milk leptin is thought to influence metabolism or the immune system in neonates, there is little information about the regulation of leptin expression in mammary epithelial cells. We examined the effect of growth hormone (GH) and/or lactogenic hormone complex (DIP; dexamethasone, insulin and prolactin) on leptin mRNA expression in mammary epithelial cells. We used a bovine mammary epithelial cell (BMEC) clonal line, which was established from a 26-day pregnant Holstein heifer. We confirmed that the mRNA was expressed in BMECs and the expression was significantly reduced by GH and/or DIP, when the cells were cultured on both plastic plates and cell culture inserts at days 2 and 7 after stimulation with lactogenic hormones. GH and/or DIP significantly increased level of α-casein mRNA in BMECs after 7 days on the cell culture inserts, but no mRNA expression was detected at day 2. GH and DIP significantly stimulated the secretion of α-casein from BMEC on cell culture inserts at 3.5 and 7 days. However, neither α-casein mRNA expression nor secretion was observed in the BMECs cultured on plastic dishes, even in the presence of GH or/and DIP. These results indicate that GH and DIP can directly reduce leptin mRNA expression in both undifferentiated and functionally differentiated bovine mammary epithelial cell.

Original languageEnglish (US)
Pages (from-to)88-96
Number of pages9
JournalDomestic Animal Endocrinology
Volume31
Issue number1
DOIs
StatePublished - Jul 1 2006

Fingerprint

Leptin
prolactin
leptin
somatotropin
Growth Hormone
breasts
Breast
epithelial cells
Epithelial Cells
Hormones
Messenger RNA
cattle
Caseins
casein
cell culture
Cell Culture Techniques
Plastics
plastics
secretion
Milk

Keywords

  • Bovine mammary epithelial cell
  • Differentiation
  • GH
  • Lactogenic hormone
  • Leptin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Endocrinology

Cite this

Growth hormone and lactogenic hormones can reduce the leptin mRNA expression in bovine mammary epithelial cells. / Yonekura, Shinichi; Sakamoto, Kazuhito; Komatsu, Tokushi; Hagino, Akihiko; Katoh, Kazuo; Obara, Yoshiaki.

In: Domestic Animal Endocrinology, Vol. 31, No. 1, 01.07.2006, p. 88-96.

Research output: Contribution to journalArticle

Yonekura, Shinichi ; Sakamoto, Kazuhito ; Komatsu, Tokushi ; Hagino, Akihiko ; Katoh, Kazuo ; Obara, Yoshiaki. / Growth hormone and lactogenic hormones can reduce the leptin mRNA expression in bovine mammary epithelial cells. In: Domestic Animal Endocrinology. 2006 ; Vol. 31, No. 1. pp. 88-96.
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