Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae

James L. Van Etten, Dwight E. Burbank, Yuannan Xia, Russel H. Meints

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Abstract

The growth and purification of milligram quantities of a large double-stranded DNA virus, PBCV-1, which replicates in a Chlorella-like alga is described. The virus had an adsorption rate constant of ca. 5 × 10-9 ml/min, a latent period of 150 to 180 min, and a burst size of 200 to 350 PFUs when the host was actively growing in the light. The eclipse period was 30 to 50 min shorter than the latent period. PBCV-1 also replicated in dark grown Chlorella but the burst size was reduced ca. 50%. The photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, had no effect on viral replication. Thus viral replication does not require host photosynthesis. Viral infection rapidly inhibited both growth and CO2 fixation of the host Chlorella.

Original languageEnglish (US)
Pages (from-to)117-125
Number of pages9
JournalVirology
Volume126
Issue number1
DOIs
StatePublished - Apr 15 1983

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Chlorella
Viruses
Growth
Diuron
DNA Viruses
Photosynthesis
Virus Diseases
Adsorption
Light
DNA

ASJC Scopus subject areas

  • Virology

Cite this

Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae. / Van Etten, James L.; Burbank, Dwight E.; Xia, Yuannan; Meints, Russel H.

In: Virology, Vol. 126, No. 1, 15.04.1983, p. 117-125.

Research output: Contribution to journalArticle

Van Etten, James L. ; Burbank, Dwight E. ; Xia, Yuannan ; Meints, Russel H. / Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae. In: Virology. 1983 ; Vol. 126, No. 1. pp. 117-125.
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