Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

The "omics" era began with transcriptomics and this progressed into proteomics. While useful, these approaches provide only circumstantial information about carbon flow, metabolic status, redox poise, etc. To more directly address these metabolic concerns, researchers have turned to the emerging field of metabolomics. In our laboratories, we frequently use NMR metabolomics to acquire a snapshot of bacterial metabolomes during stressful or transition events. Irrespective of the "omics" method of choice, the experimental outcome depends on the proper cultivation and preparation of bacterial samples. In addition, the integration of these large datasets requires that these cultivation conditions be clearly defined [1].

Original languageEnglish (US)
Title of host publicationStaphylococcus Epidermidis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages71-91
Number of pages21
ISBN (Print)9781627037358
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1106
ISSN (Print)1064-3745

Fingerprint

Metabolomics
Staphylococcus epidermidis
Metabolome
Growth
Proteomics
Oxidation-Reduction
Carbon
Research Personnel
Datasets

Keywords

  • Bacterial growth
  • Growth conditions
  • Metabolism
  • Metabolomics
  • NMR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Somerville, G. A., & Powers, R. (2014). Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis. In Staphylococcus Epidermidis: Methods and Protocols (pp. 71-91). (Methods in Molecular Biology; Vol. 1106). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-736-5_6

Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis. / Somerville, Greg A; Powers, Robert.

Staphylococcus Epidermidis: Methods and Protocols. Humana Press Inc., 2014. p. 71-91 (Methods in Molecular Biology; Vol. 1106).

Research output: Chapter in Book/Report/Conference proceedingChapter

Somerville, GA & Powers, R 2014, Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis. in Staphylococcus Epidermidis: Methods and Protocols. Methods in Molecular Biology, vol. 1106, Humana Press Inc., pp. 71-91. https://doi.org/10.1007/978-1-62703-736-5_6
Somerville GA, Powers R. Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis. In Staphylococcus Epidermidis: Methods and Protocols. Humana Press Inc. 2014. p. 71-91. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-736-5_6
Somerville, Greg A ; Powers, Robert. / Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis. Staphylococcus Epidermidis: Methods and Protocols. Humana Press Inc., 2014. pp. 71-91 (Methods in Molecular Biology).
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