Group B coxsackievirus myocarditis and pancreatitis: Connection between viral virulence phenotypes in mice

Steven Tracy, Katja Höfling, Samuel Jay Pirruccello, Pascale H. Lane, Sara M. Reyna, Charles J. Gauntt

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The group B coxsackieviruses (CVB) induce experimental pancreatitis and myocarditis in mice and are established agents of human myocarditis, especially in children. We tested the hypothesis that the development of CVB-induced myocarditis is linked to CVB-induced pancreatitis by studying the replication of different CVB strains in mice. Eight of nine genotypically different type 3 CVB (CVB3) strains induced acute pancreatitis in mice; of these, three viruses also induced acute myocarditis. One CVB3 strain was avirulent for both organs. Myocarditis was not observed in the absence of pancreatitis. The results obtained by inoculation of mice with strains of other CVB serotypes were consistent with these data. Infectious virus titers were measured in serum, pancreas, and heart as a function of time after inoculation of mice with three CVB3 strains. Each strain was representative of one of the three viral virulence phenotypes: avirulent, pancreovirulent only, and cardiovirulent. All strains replicated well and persisted in the pancreas through 8 days post-inoculation, but the cardiovirulent CVB3 strain tended to replicate to higher titer earlier and persist longer in sera, pancreatic, and cardiac tissues than the noncardiovirulent strains. Replication of the CVB3 strains were studied in two human pancreatic tumor lines and in primary human endothelial cell cultures derived from cardiac artery. Cardiovirulent strains, both individually and as a group, tended to replicate to titers as high as, or higher than, noncardiovirulent strains did in cell culture. The data are consistent with the possibility of an etiologic link between CVB-induced pancreatic and heart disease. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)70-81
Number of pages12
JournalJournal of Medical Virology
Volume62
Issue number1
DOIs
StatePublished - Jan 1 2000

Fingerprint

Human Enterovirus B
Myocarditis
Pancreatitis
Virulence
Phenotype
Pancreas
Cell Culture Techniques
Pancreatic Diseases
Viral Load
Serum
Heart Diseases
Endothelial Cells
Arteries
Viruses
Neoplasms

Keywords

  • Group B CVB
  • Myocarditis
  • Pancreatitis

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Group B coxsackievirus myocarditis and pancreatitis : Connection between viral virulence phenotypes in mice. / Tracy, Steven; Höfling, Katja; Pirruccello, Samuel Jay; Lane, Pascale H.; Reyna, Sara M.; Gauntt, Charles J.

In: Journal of Medical Virology, Vol. 62, No. 1, 01.01.2000, p. 70-81.

Research output: Contribution to journalArticle

Tracy, Steven ; Höfling, Katja ; Pirruccello, Samuel Jay ; Lane, Pascale H. ; Reyna, Sara M. ; Gauntt, Charles J. / Group B coxsackievirus myocarditis and pancreatitis : Connection between viral virulence phenotypes in mice. In: Journal of Medical Virology. 2000 ; Vol. 62, No. 1. pp. 70-81.
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