Greater importance of Ca2+-calmodulin in maintenance of ang II-and K+-mediated aldosterone secretion

Lesser role of protein kinase C

Arunabha Ganguly, Shirley Chiou, Naomi S. Fineberg, John S Davis

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In this study we have investigated various components of the stimulus-secretion coupling process leading to aldosterone secretion from the calf adrenal glomerulosa cells as evoked by angiotensin II (AII) and potassium (K+). The roles of Ca2+, calmodulin and protein kinase C in the sustained phase rather than initiation of aldosterone secretion were of special interest. Our investigations revealed that the reduction of extracellular Ca2+ by EGTA or interruption of Ca2+ influx by nitrendipine at various time points after stimulation with either AII or K+ markedly compromised aldosterone secretion. Calmodulin inhibitors, calmidazolium and W-7 reduced aldosterone secretion profoundly. Agonists of protein kinase C, phorbol ester or diacylglycerol analogues failed to stimulate aldosterone secretion while the protein kinase C inhibitor, H-7, only partially inhibited aldosterone secretion at a concentration which completely inhibited protein kinase C activity. Calmodulin inhibitors produced significantly greater inhibition of aldosterone secretion than inhibitors of protein kinase C.

Original languageEnglish (US)
Pages (from-to)254-261
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number1
DOIs
StatePublished - Jan 15 1992

Fingerprint

Calmodulin
Aldosterone
Protein Kinase C
Maintenance
Angiotensin II
calmidazolium
Zona Glomerulosa
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Nitrendipine
Calcium-Calmodulin-Dependent Protein Kinases
Protein C Inhibitor
Secretory Pathway
Egtazic Acid
Diglycerides
Phorbol Esters
Protein Kinase Inhibitors
Potassium

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Greater importance of Ca2+-calmodulin in maintenance of ang II-and K+-mediated aldosterone secretion : Lesser role of protein kinase C. / Ganguly, Arunabha; Chiou, Shirley; Fineberg, Naomi S.; Davis, John S.

In: Biochemical and Biophysical Research Communications, Vol. 182, No. 1, 15.01.1992, p. 254-261.

Research output: Contribution to journalArticle

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