Grain dusts and grain plant components vary in their ability to recruit neutrophils

Susanna G Von Essen, D. P. O'Neill, S. A. Olenchok, R. A. Robbins, S. I. Rennard

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Occupational exposure to grain dusts can cause bronchitis, particularly to grain sorghum dust. Bronchitis is associated with the presence of increased numbers of neutrophils. To determine how grain dusts could cause neutrophil recruitment to the airways, extract of whole-grain sorghum, corn, and soybean dusts and of pulverized components of these plants were made with Hanks balanced salt solution (HBSS) and used in direct neutrophil chemotaxis experiments. The glume of the grain sorghum plant, the structure holding the seeds in place, caused the migration of the greatest number of neutrophils compared to HBSS [132 ±7 vs. 60 cells/high-power field (hpf) ± 2 SEM, p <.001J, followed by whole- grain sorghum dust (121 ±5 cells/hpf). Next, bovine bronchial epithelial cells (BBECs) were obtained from fresh lungs and grown to near confluence before challenge with a 10% solution of grain dust and grain plant extracts. The grain sorghum glume and whole-grain sorghum dusts caused release of the greatest amount of neutrophil chemotactic activity (NCA) from BBECs compared to the medium M199 negative control (141 ± 6 and 15 ± 7, respectively, vs. 64 cells/hpf ± 3 SEM, p <.001). The ability to cause neutrophils to migrate by direct and indirect means did not correlate with levels in the grain dusts of endotoxin, which is known to cause release of NCA from bronchial epithelial cells. Therefore, this article describes additional mechanisms by which grain dusts can cause pulmonary inflammation and that are independent of endotoxin levels.

Original languageEnglish (US)
Pages (from-to)425-441
Number of pages17
JournalJournal of Toxicology and Environmental Health
Volume46
Issue number4
DOIs
StatePublished - Dec 1995

Fingerprint

Plant Structures
Aptitude
Dust
Neutrophils
Sorghum
Bronchitis
Epithelial Cells
Endotoxins
Neutrophil Infiltration
Plant Extracts
Chemotaxis
Plant extracts
Occupational Exposure
Salts
Soybeans
Scanning electron microscopy
Zea mays
Seeds
Pneumonia
Seed

ASJC Scopus subject areas

  • Toxicology
  • Pollution

Cite this

Grain dusts and grain plant components vary in their ability to recruit neutrophils. / Von Essen, Susanna G; O'Neill, D. P.; Olenchok, S. A.; Robbins, R. A.; Rennard, S. I.

In: Journal of Toxicology and Environmental Health, Vol. 46, No. 4, 12.1995, p. 425-441.

Research output: Contribution to journalArticle

Von Essen, Susanna G ; O'Neill, D. P. ; Olenchok, S. A. ; Robbins, R. A. ; Rennard, S. I. / Grain dusts and grain plant components vary in their ability to recruit neutrophils. In: Journal of Toxicology and Environmental Health. 1995 ; Vol. 46, No. 4. pp. 425-441.
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