Gradient temperature hybridization using a thermocycler for RNase protection assays

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8 Citations (Scopus)

Abstract

The RNase protection assay (RPA) is a sensitive and quantitative assay for the detection of specific RNAs. Because some probes require an optimal temperature for efficient hybridization to the target RNAs, preliminary experiments to determine the optimal temperatures have to be done empirically, a process that is laborious and time-consuming. In order to simplify this process, a new gradient temperature hybridization procedure has been developed with the use of a thermocycler. This procedure eliminates the need for preliminary experiments to determine the optimal temperature of hybridization for a given probe.

Original languageEnglish (US)
Pages (from-to)73-75
Number of pages3
JournalApplied Biochemistry and Biotechnology - Part B Molecular Biotechnology
Volume14
Issue number1
StatePublished - May 30 2000

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Ribonucleases
Thermal gradients
Assays
RNA
Temperature
Experiments

Keywords

  • RPA
  • Thermocycler

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

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