Graded hypoxia and blood oxidative stress during exercise recovery

Bridget Peters, Christopher Ballmann, Graham Mcginnis, Erin Epstein, Hayden Hyatt, Dustin Slivka, John Cuddy, William Hailes, Charles Dumke, Brent Ruby, John Quindry

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Altitude exposure and exercise elicit oxidative stress in blood; however, exercise recovery at 5000 m attenuates oxidative stress. The purpose was to determine the altitude threshold at which blood oxidative stress is blunted during exercise recovery. Twelve males 18-28 years performed four-cycle ergometry bouts (60 min, 70% VO2max, at 975 m). In a randomised counterbalanced crossover design, participants recovered 6 h at 0, 1667, 3333 and 5000 m in a normobaric hypoxia chamber (recovery altitudes were simulated by using a computerised system in an environmental chamber by lowering the partial pressure of oxygen to match that of the respective altitude). Oxygen saturation was monitored throughout exercise recovery. Blood samples obtained pre-, post-, 1 h post- and 5 h post-exercise were assayed for ferricreducing antioxidant plasma, Trolox equivalent antioxidant capacity, uric acid, lipid hydroperoxides and protein carbonyls. Muscle biopsies obtained pre and 6 h were analysed by real-time polymerase chain reaction to quantify expression of hemeoxgenase 1, superoxide dismutase 2 and nuclear factor (euthyroid-derived 2)-like factor. Pulse oximetry data were similar during exercise, but decreased for the three highest recovery elevations (0 m = 0%, 1667 m = -3%; 3333 m = -7%; 5000 m = -17%). A time-dependent oxidative stress occurred following exercise for all variables, but the two highest recovery altitudes partially attenuated the lipid hydroperoxide response (0 m = +135%, 1667 m = +251%, 3333 m = +99%; 5000 m = +108%). Data may indicate an altitude threshold between 1667 and 3333 m, above which the oxidative stress response is blunted during exercise recovery.

    Original languageEnglish (US)
    Pages (from-to)56-66
    Number of pages11
    JournalJournal of Sports Sciences
    Volume34
    Issue number1
    DOIs
    StatePublished - Jan 1 2016

    Fingerprint

    Oxidative Stress
    Lipid Peroxides
    Antioxidants
    Oxygen
    Ergometry
    Oximetry
    Partial Pressure
    Uric Acid
    Cross-Over Studies
    Hypoxia
    Real-Time Polymerase Chain Reaction
    Biopsy
    Muscles
    Proteins

    Keywords

    • Altitude
    • Exercise
    • Oxidative stress
    • Reactive oxygen species

    ASJC Scopus subject areas

    • Orthopedics and Sports Medicine
    • Physical Therapy, Sports Therapy and Rehabilitation

    Cite this

    Peters, B., Ballmann, C., Mcginnis, G., Epstein, E., Hyatt, H., Slivka, D., ... Quindry, J. (2016). Graded hypoxia and blood oxidative stress during exercise recovery. Journal of Sports Sciences, 34(1), 56-66. https://doi.org/10.1080/02640414.2015.1031164

    Graded hypoxia and blood oxidative stress during exercise recovery. / Peters, Bridget; Ballmann, Christopher; Mcginnis, Graham; Epstein, Erin; Hyatt, Hayden; Slivka, Dustin; Cuddy, John; Hailes, William; Dumke, Charles; Ruby, Brent; Quindry, John.

    In: Journal of Sports Sciences, Vol. 34, No. 1, 01.01.2016, p. 56-66.

    Research output: Contribution to journalArticle

    Peters, B, Ballmann, C, Mcginnis, G, Epstein, E, Hyatt, H, Slivka, D, Cuddy, J, Hailes, W, Dumke, C, Ruby, B & Quindry, J 2016, 'Graded hypoxia and blood oxidative stress during exercise recovery', Journal of Sports Sciences, vol. 34, no. 1, pp. 56-66. https://doi.org/10.1080/02640414.2015.1031164
    Peters, Bridget ; Ballmann, Christopher ; Mcginnis, Graham ; Epstein, Erin ; Hyatt, Hayden ; Slivka, Dustin ; Cuddy, John ; Hailes, William ; Dumke, Charles ; Ruby, Brent ; Quindry, John. / Graded hypoxia and blood oxidative stress during exercise recovery. In: Journal of Sports Sciences. 2016 ; Vol. 34, No. 1. pp. 56-66.
    @article{8748eaca12eb456a8ea710ba91bb1233,
    title = "Graded hypoxia and blood oxidative stress during exercise recovery",
    abstract = "Altitude exposure and exercise elicit oxidative stress in blood; however, exercise recovery at 5000 m attenuates oxidative stress. The purpose was to determine the altitude threshold at which blood oxidative stress is blunted during exercise recovery. Twelve males 18-28 years performed four-cycle ergometry bouts (60 min, 70{\%} VO2max, at 975 m). In a randomised counterbalanced crossover design, participants recovered 6 h at 0, 1667, 3333 and 5000 m in a normobaric hypoxia chamber (recovery altitudes were simulated by using a computerised system in an environmental chamber by lowering the partial pressure of oxygen to match that of the respective altitude). Oxygen saturation was monitored throughout exercise recovery. Blood samples obtained pre-, post-, 1 h post- and 5 h post-exercise were assayed for ferricreducing antioxidant plasma, Trolox equivalent antioxidant capacity, uric acid, lipid hydroperoxides and protein carbonyls. Muscle biopsies obtained pre and 6 h were analysed by real-time polymerase chain reaction to quantify expression of hemeoxgenase 1, superoxide dismutase 2 and nuclear factor (euthyroid-derived 2)-like factor. Pulse oximetry data were similar during exercise, but decreased for the three highest recovery elevations (0 m = 0{\%}, 1667 m = -3{\%}; 3333 m = -7{\%}; 5000 m = -17{\%}). A time-dependent oxidative stress occurred following exercise for all variables, but the two highest recovery altitudes partially attenuated the lipid hydroperoxide response (0 m = +135{\%}, 1667 m = +251{\%}, 3333 m = +99{\%}; 5000 m = +108{\%}). Data may indicate an altitude threshold between 1667 and 3333 m, above which the oxidative stress response is blunted during exercise recovery.",
    keywords = "Altitude, Exercise, Oxidative stress, Reactive oxygen species",
    author = "Bridget Peters and Christopher Ballmann and Graham Mcginnis and Erin Epstein and Hayden Hyatt and Dustin Slivka and John Cuddy and William Hailes and Charles Dumke and Brent Ruby and John Quindry",
    year = "2016",
    month = "1",
    day = "1",
    doi = "10.1080/02640414.2015.1031164",
    language = "English (US)",
    volume = "34",
    pages = "56--66",
    journal = "Journal of Sports Sciences",
    issn = "0264-0414",
    publisher = "Routledge",
    number = "1",

    }

    TY - JOUR

    T1 - Graded hypoxia and blood oxidative stress during exercise recovery

    AU - Peters, Bridget

    AU - Ballmann, Christopher

    AU - Mcginnis, Graham

    AU - Epstein, Erin

    AU - Hyatt, Hayden

    AU - Slivka, Dustin

    AU - Cuddy, John

    AU - Hailes, William

    AU - Dumke, Charles

    AU - Ruby, Brent

    AU - Quindry, John

    PY - 2016/1/1

    Y1 - 2016/1/1

    N2 - Altitude exposure and exercise elicit oxidative stress in blood; however, exercise recovery at 5000 m attenuates oxidative stress. The purpose was to determine the altitude threshold at which blood oxidative stress is blunted during exercise recovery. Twelve males 18-28 years performed four-cycle ergometry bouts (60 min, 70% VO2max, at 975 m). In a randomised counterbalanced crossover design, participants recovered 6 h at 0, 1667, 3333 and 5000 m in a normobaric hypoxia chamber (recovery altitudes were simulated by using a computerised system in an environmental chamber by lowering the partial pressure of oxygen to match that of the respective altitude). Oxygen saturation was monitored throughout exercise recovery. Blood samples obtained pre-, post-, 1 h post- and 5 h post-exercise were assayed for ferricreducing antioxidant plasma, Trolox equivalent antioxidant capacity, uric acid, lipid hydroperoxides and protein carbonyls. Muscle biopsies obtained pre and 6 h were analysed by real-time polymerase chain reaction to quantify expression of hemeoxgenase 1, superoxide dismutase 2 and nuclear factor (euthyroid-derived 2)-like factor. Pulse oximetry data were similar during exercise, but decreased for the three highest recovery elevations (0 m = 0%, 1667 m = -3%; 3333 m = -7%; 5000 m = -17%). A time-dependent oxidative stress occurred following exercise for all variables, but the two highest recovery altitudes partially attenuated the lipid hydroperoxide response (0 m = +135%, 1667 m = +251%, 3333 m = +99%; 5000 m = +108%). Data may indicate an altitude threshold between 1667 and 3333 m, above which the oxidative stress response is blunted during exercise recovery.

    AB - Altitude exposure and exercise elicit oxidative stress in blood; however, exercise recovery at 5000 m attenuates oxidative stress. The purpose was to determine the altitude threshold at which blood oxidative stress is blunted during exercise recovery. Twelve males 18-28 years performed four-cycle ergometry bouts (60 min, 70% VO2max, at 975 m). In a randomised counterbalanced crossover design, participants recovered 6 h at 0, 1667, 3333 and 5000 m in a normobaric hypoxia chamber (recovery altitudes were simulated by using a computerised system in an environmental chamber by lowering the partial pressure of oxygen to match that of the respective altitude). Oxygen saturation was monitored throughout exercise recovery. Blood samples obtained pre-, post-, 1 h post- and 5 h post-exercise were assayed for ferricreducing antioxidant plasma, Trolox equivalent antioxidant capacity, uric acid, lipid hydroperoxides and protein carbonyls. Muscle biopsies obtained pre and 6 h were analysed by real-time polymerase chain reaction to quantify expression of hemeoxgenase 1, superoxide dismutase 2 and nuclear factor (euthyroid-derived 2)-like factor. Pulse oximetry data were similar during exercise, but decreased for the three highest recovery elevations (0 m = 0%, 1667 m = -3%; 3333 m = -7%; 5000 m = -17%). A time-dependent oxidative stress occurred following exercise for all variables, but the two highest recovery altitudes partially attenuated the lipid hydroperoxide response (0 m = +135%, 1667 m = +251%, 3333 m = +99%; 5000 m = +108%). Data may indicate an altitude threshold between 1667 and 3333 m, above which the oxidative stress response is blunted during exercise recovery.

    KW - Altitude

    KW - Exercise

    KW - Oxidative stress

    KW - Reactive oxygen species

    UR - http://www.scopus.com/inward/record.url?scp=84927747493&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84927747493&partnerID=8YFLogxK

    U2 - 10.1080/02640414.2015.1031164

    DO - 10.1080/02640414.2015.1031164

    M3 - Article

    C2 - 25871479

    AN - SCOPUS:84927747493

    VL - 34

    SP - 56

    EP - 66

    JO - Journal of Sports Sciences

    JF - Journal of Sports Sciences

    SN - 0264-0414

    IS - 1

    ER -