Graded hypoxia and blood oxidative stress during exercise recovery

Bridget Peters, Christopher Ballmann, Graham Mcginnis, Erin Epstein, Hayden Hyatt, Dustin Slivka, John Cuddy, William Hailes, Charles Dumke, Brent Ruby, John Quindry

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    Abstract

    Altitude exposure and exercise elicit oxidative stress in blood; however, exercise recovery at 5000 m attenuates oxidative stress. The purpose was to determine the altitude threshold at which blood oxidative stress is blunted during exercise recovery. Twelve males 18-28 years performed four-cycle ergometry bouts (60 min, 70% VO2max, at 975 m). In a randomised counterbalanced crossover design, participants recovered 6 h at 0, 1667, 3333 and 5000 m in a normobaric hypoxia chamber (recovery altitudes were simulated by using a computerised system in an environmental chamber by lowering the partial pressure of oxygen to match that of the respective altitude). Oxygen saturation was monitored throughout exercise recovery. Blood samples obtained pre-, post-, 1 h post- and 5 h post-exercise were assayed for ferricreducing antioxidant plasma, Trolox equivalent antioxidant capacity, uric acid, lipid hydroperoxides and protein carbonyls. Muscle biopsies obtained pre and 6 h were analysed by real-time polymerase chain reaction to quantify expression of hemeoxgenase 1, superoxide dismutase 2 and nuclear factor (euthyroid-derived 2)-like factor. Pulse oximetry data were similar during exercise, but decreased for the three highest recovery elevations (0 m = 0%, 1667 m = -3%; 3333 m = -7%; 5000 m = -17%). A time-dependent oxidative stress occurred following exercise for all variables, but the two highest recovery altitudes partially attenuated the lipid hydroperoxide response (0 m = +135%, 1667 m = +251%, 3333 m = +99%; 5000 m = +108%). Data may indicate an altitude threshold between 1667 and 3333 m, above which the oxidative stress response is blunted during exercise recovery.

    Original languageEnglish (US)
    Pages (from-to)56-66
    Number of pages11
    JournalJournal of Sports Sciences
    Volume34
    Issue number1
    DOIs
    StatePublished - Jan 1 2016

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    Keywords

    • Altitude
    • Exercise
    • Oxidative stress
    • Reactive oxygen species

    ASJC Scopus subject areas

    • Orthopedics and Sports Medicine
    • Physical Therapy, Sports Therapy and Rehabilitation

    Cite this

    Peters, B., Ballmann, C., Mcginnis, G., Epstein, E., Hyatt, H., Slivka, D., Cuddy, J., Hailes, W., Dumke, C., Ruby, B., & Quindry, J. (2016). Graded hypoxia and blood oxidative stress during exercise recovery. Journal of Sports Sciences, 34(1), 56-66. https://doi.org/10.1080/02640414.2015.1031164