GM-CSF-mobilized peripheral blood CD34+ cells differ from steady-state bone marrow CD34+ cells in adhesion molecule expression

T. Watanabe, Bhavana J Dave, D. G. Heimann, E. Lethaby, A. Kessinger, James E Talmadge

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

To determine the effect of growth factor mobilization on the expression of adhesion molecules, we compared CD34+ progenitor cell (PC) populations from steady-state bone marrow (BM) with granulocyte-macrophage colony-stimulating factor (GM-CSF)-mobilized apheresis products (peripheral blood stem cell (PSC)) using flow cytometry. To increase the accuracy of this analysis, CD34+ cells were enriched (MiniMACS) before cytometric analysis. A significantly lower expression of very late antigen-4 (VLA-4), leukocyte function antigen-1 (LFA-1) and LFA-3 were observed on PSC compared to BM CD34+ cells. In addition, significantly lower mean fluorescence intensity (MFI) of VLA-4, VLA-5, intercellular adhesion molecule-1 (ICAM-1), and sialyl Lewis(x) were observed on PSC as compared to BM CD34+ cells. Significantly higher levels of L-selectin and CD44 expression were observed on PSC as compared to BM CD34+ cells based on frequency and MFI (P ≤ 0.05). In addition, the duration of GM-CSF administration or number of prior aphereses had no effect on adhesion molecule expression. These data suggest that decreased expression of adhesion molecules including VLA-4, LFA-1, ICAM-1 and LFA-3 play a role in PC mobilization. Based on these studies, we suggest that PC mobilization occurs as a stochastic process and is associated with the selection of CD34+ cells with low adhesion molecule expression.

Original languageEnglish (US)
Pages (from-to)1175-1181
Number of pages7
JournalBone marrow transplantation
Volume19
Issue number12
DOIs
StatePublished - Jun 2 1997

Fingerprint

Cell Adhesion Molecules
Granulocyte-Macrophage Colony-Stimulating Factor
Integrin alpha4beta1
Bone Marrow Cells
Blood Cells
CD58 Antigens
Blood Component Removal
Stem Cells
Intercellular Adhesion Molecule-1
HLA Antigens
Fluorescence
Integrin alpha5beta1
Stochastic Processes
L-Selectin
Intercellular Signaling Peptides and Proteins
Flow Cytometry
Bone Marrow
Peripheral Blood Stem Cells
Population

Keywords

  • Adhesion molecule
  • Bone marrow CD34 cells
  • GM-CSF mobilization
  • Peripheral blood CD34 cells

ASJC Scopus subject areas

  • Hematology
  • Transplantation

Cite this

GM-CSF-mobilized peripheral blood CD34+ cells differ from steady-state bone marrow CD34+ cells in adhesion molecule expression. / Watanabe, T.; Dave, Bhavana J; Heimann, D. G.; Lethaby, E.; Kessinger, A.; Talmadge, James E.

In: Bone marrow transplantation, Vol. 19, No. 12, 02.06.1997, p. 1175-1181.

Research output: Contribution to journalArticle

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abstract = "To determine the effect of growth factor mobilization on the expression of adhesion molecules, we compared CD34+ progenitor cell (PC) populations from steady-state bone marrow (BM) with granulocyte-macrophage colony-stimulating factor (GM-CSF)-mobilized apheresis products (peripheral blood stem cell (PSC)) using flow cytometry. To increase the accuracy of this analysis, CD34+ cells were enriched (MiniMACS) before cytometric analysis. A significantly lower expression of very late antigen-4 (VLA-4), leukocyte function antigen-1 (LFA-1) and LFA-3 were observed on PSC compared to BM CD34+ cells. In addition, significantly lower mean fluorescence intensity (MFI) of VLA-4, VLA-5, intercellular adhesion molecule-1 (ICAM-1), and sialyl Lewis(x) were observed on PSC as compared to BM CD34+ cells. Significantly higher levels of L-selectin and CD44 expression were observed on PSC as compared to BM CD34+ cells based on frequency and MFI (P ≤ 0.05). In addition, the duration of GM-CSF administration or number of prior aphereses had no effect on adhesion molecule expression. These data suggest that decreased expression of adhesion molecules including VLA-4, LFA-1, ICAM-1 and LFA-3 play a role in PC mobilization. Based on these studies, we suggest that PC mobilization occurs as a stochastic process and is associated with the selection of CD34+ cells with low adhesion molecule expression.",
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