Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation

H. Zhou, J. W. Arrowsmith, Michael E Fromm, C. M. Hironaka, M. L. Taylor, D. Rodriguez, M. E. Pajeau, S. M. Brown, C. G. Santino, J. E. Fry

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The lack of alternative selectable markers in crop transformation has been a substantial barrier for commercial application of agricultural biotechnology. We have developed an efficient selection system for wheat transformation using glyphosate-tolerant CP4 and GOX genes as a selectable marker. Immature embryos of the wheat cultivar Bobwhite were bombarded with two separate plasmids harboring the CP4/GOX and GUS genes. After a 1 week delay, the bombarded embryos were transferred to a selection medium containing 2 mM glyphosate. Embryo-derived calli were subcultured onto the same selection medium every 3 weeks consecutively for 9-12 weeks, and were then regenerated and rooted on selection media with lower glyphosate concentrations. Transgenic plants tolerant to glyphosate were recovered. ELISA assay confirmed expression of the CP4 and GOX genes in R0 plants. Southern blot analysis demonstrated that the transgenes were integrated into the wheat genomes and transmitted to the following generation. The use of CP4 and GOX genes as a selectable marker provides an efficient, effective, and alternative transformation selection system for wheat.

Original languageEnglish (US)
Pages (from-to)159-163
Number of pages5
JournalPlant Cell Reports
Volume15
Issue number3-4
DOIs
StatePublished - Dec 1 1995

Fingerprint

glyphosate
embryo (plant)
wheat
genes
agricultural biotechnology
Southern blotting
transgenes
transgenic plants
plasmids
callus
immatures
enzyme-linked immunosorbent assay
genome
cultivars
assays
crops

Keywords

  • CP4/GOX
  • Wheat
  • embryos
  • glyphosate
  • transformation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Zhou, H., Arrowsmith, J. W., Fromm, M. E., Hironaka, C. M., Taylor, M. L., Rodriguez, D., ... Fry, J. E. (1995). Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Reports, 15(3-4), 159-163. https://doi.org/10.1007/BF00193711

Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. / Zhou, H.; Arrowsmith, J. W.; Fromm, Michael E; Hironaka, C. M.; Taylor, M. L.; Rodriguez, D.; Pajeau, M. E.; Brown, S. M.; Santino, C. G.; Fry, J. E.

In: Plant Cell Reports, Vol. 15, No. 3-4, 01.12.1995, p. 159-163.

Research output: Contribution to journalArticle

Zhou, H, Arrowsmith, JW, Fromm, ME, Hironaka, CM, Taylor, ML, Rodriguez, D, Pajeau, ME, Brown, SM, Santino, CG & Fry, JE 1995, 'Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation', Plant Cell Reports, vol. 15, no. 3-4, pp. 159-163. https://doi.org/10.1007/BF00193711
Zhou H, Arrowsmith JW, Fromm ME, Hironaka CM, Taylor ML, Rodriguez D et al. Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Reports. 1995 Dec 1;15(3-4):159-163. https://doi.org/10.1007/BF00193711
Zhou, H. ; Arrowsmith, J. W. ; Fromm, Michael E ; Hironaka, C. M. ; Taylor, M. L. ; Rodriguez, D. ; Pajeau, M. E. ; Brown, S. M. ; Santino, C. G. ; Fry, J. E. / Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. In: Plant Cell Reports. 1995 ; Vol. 15, No. 3-4. pp. 159-163.
@article{f9331de59c524b8f8f2017e7f9bce16c,
title = "Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation",
abstract = "The lack of alternative selectable markers in crop transformation has been a substantial barrier for commercial application of agricultural biotechnology. We have developed an efficient selection system for wheat transformation using glyphosate-tolerant CP4 and GOX genes as a selectable marker. Immature embryos of the wheat cultivar Bobwhite were bombarded with two separate plasmids harboring the CP4/GOX and GUS genes. After a 1 week delay, the bombarded embryos were transferred to a selection medium containing 2 mM glyphosate. Embryo-derived calli were subcultured onto the same selection medium every 3 weeks consecutively for 9-12 weeks, and were then regenerated and rooted on selection media with lower glyphosate concentrations. Transgenic plants tolerant to glyphosate were recovered. ELISA assay confirmed expression of the CP4 and GOX genes in R0 plants. Southern blot analysis demonstrated that the transgenes were integrated into the wheat genomes and transmitted to the following generation. The use of CP4 and GOX genes as a selectable marker provides an efficient, effective, and alternative transformation selection system for wheat.",
keywords = "CP4/GOX, Wheat, embryos, glyphosate, transformation",
author = "H. Zhou and Arrowsmith, {J. W.} and Fromm, {Michael E} and Hironaka, {C. M.} and Taylor, {M. L.} and D. Rodriguez and Pajeau, {M. E.} and Brown, {S. M.} and Santino, {C. G.} and Fry, {J. E.}",
year = "1995",
month = "12",
day = "1",
doi = "10.1007/BF00193711",
language = "English (US)",
volume = "15",
pages = "159--163",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "3-4",

}

TY - JOUR

T1 - Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation

AU - Zhou, H.

AU - Arrowsmith, J. W.

AU - Fromm, Michael E

AU - Hironaka, C. M.

AU - Taylor, M. L.

AU - Rodriguez, D.

AU - Pajeau, M. E.

AU - Brown, S. M.

AU - Santino, C. G.

AU - Fry, J. E.

PY - 1995/12/1

Y1 - 1995/12/1

N2 - The lack of alternative selectable markers in crop transformation has been a substantial barrier for commercial application of agricultural biotechnology. We have developed an efficient selection system for wheat transformation using glyphosate-tolerant CP4 and GOX genes as a selectable marker. Immature embryos of the wheat cultivar Bobwhite were bombarded with two separate plasmids harboring the CP4/GOX and GUS genes. After a 1 week delay, the bombarded embryos were transferred to a selection medium containing 2 mM glyphosate. Embryo-derived calli were subcultured onto the same selection medium every 3 weeks consecutively for 9-12 weeks, and were then regenerated and rooted on selection media with lower glyphosate concentrations. Transgenic plants tolerant to glyphosate were recovered. ELISA assay confirmed expression of the CP4 and GOX genes in R0 plants. Southern blot analysis demonstrated that the transgenes were integrated into the wheat genomes and transmitted to the following generation. The use of CP4 and GOX genes as a selectable marker provides an efficient, effective, and alternative transformation selection system for wheat.

AB - The lack of alternative selectable markers in crop transformation has been a substantial barrier for commercial application of agricultural biotechnology. We have developed an efficient selection system for wheat transformation using glyphosate-tolerant CP4 and GOX genes as a selectable marker. Immature embryos of the wheat cultivar Bobwhite were bombarded with two separate plasmids harboring the CP4/GOX and GUS genes. After a 1 week delay, the bombarded embryos were transferred to a selection medium containing 2 mM glyphosate. Embryo-derived calli were subcultured onto the same selection medium every 3 weeks consecutively for 9-12 weeks, and were then regenerated and rooted on selection media with lower glyphosate concentrations. Transgenic plants tolerant to glyphosate were recovered. ELISA assay confirmed expression of the CP4 and GOX genes in R0 plants. Southern blot analysis demonstrated that the transgenes were integrated into the wheat genomes and transmitted to the following generation. The use of CP4 and GOX genes as a selectable marker provides an efficient, effective, and alternative transformation selection system for wheat.

KW - CP4/GOX

KW - Wheat

KW - embryos

KW - glyphosate

KW - transformation

UR - http://www.scopus.com/inward/record.url?scp=0029201349&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029201349&partnerID=8YFLogxK

U2 - 10.1007/BF00193711

DO - 10.1007/BF00193711

M3 - Article

VL - 15

SP - 159

EP - 163

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 3-4

ER -