Glyphosate as a selective agent for the production of fertile transgenic maize (Zea mays L.) plants

Arlene R. Howe, Charles S. Gasser, Sherri M. Brown, Stephen R. Padgette, Jesse Hart, Gregory B. Parker, Michael E Fromm, Charles L. Armstrong

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Efficient and reproducible selection of transgenic cells is an essential component of a good transformation system. In this paper, we describe the development of glyphosate as a selective agent for the recovery of transgenic embryogenic corn callus and the production of plants tolerant to Roundup® herbicide. Glyphosate, the active ingredient in Roundup® herbicide inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and thus prevents the synthesis of chorismate-derived aromatic amino acids and secondary metabolites in plants. A maize EPSPS gene has been cloned, mutated to produce a modified enzyme resistant to inhibition by glyphosate, and engineered into a monocot expression vector. In addition, a bacterial gene which degrades glyphosate (glyphosate oxidoreductase, or GOX) was also cloned into a similar expression vector. Stably transformed callus has been reproducibly recovered following introduction of mutant maize EPSPS and GOX genes into tissue culture cells by particle bombardment and selection on glyphosate-containing medium. Plants have been regenerated both on and off glyphosate selection medium, and are tolerant to normally lethal levels of Roundup®. Excellent seed set has been obtained from both self and outcross pollinations from both sprayed and unsprayed regenerated plants. Progeny tests have demonstrated normal Mendelian transmission and tolerance to the herbicide for some of the transgenic events.

Original languageEnglish (US)
Pages (from-to)153-164
Number of pages12
JournalMolecular Breeding
Volume10
Issue number3
DOIs
StatePublished - Oct 30 2002

Fingerprint

glyphosate
Zea mays
genetically modified organisms
corn
herbicides
Herbicides
callus
Genes
Bony Callus
phosphates
genes
3-Phosphoshikimate 1-Carboxyvinyltransferase
oxidoreductases
Phosphates
enzymes
Liliopsida
active ingredients
seed set
lethal genes
secondary metabolites

Keywords

  • 5-enolpyruvylshikimate-3-phosphate synthase
  • Glyphosate
  • Maize transformation
  • Selectable marker

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Howe, A. R., Gasser, C. S., Brown, S. M., Padgette, S. R., Hart, J., Parker, G. B., ... Armstrong, C. L. (2002). Glyphosate as a selective agent for the production of fertile transgenic maize (Zea mays L.) plants. Molecular Breeding, 10(3), 153-164. https://doi.org/10.1023/A:1020396708088

Glyphosate as a selective agent for the production of fertile transgenic maize (Zea mays L.) plants. / Howe, Arlene R.; Gasser, Charles S.; Brown, Sherri M.; Padgette, Stephen R.; Hart, Jesse; Parker, Gregory B.; Fromm, Michael E; Armstrong, Charles L.

In: Molecular Breeding, Vol. 10, No. 3, 30.10.2002, p. 153-164.

Research output: Contribution to journalArticle

Howe, AR, Gasser, CS, Brown, SM, Padgette, SR, Hart, J, Parker, GB, Fromm, ME & Armstrong, CL 2002, 'Glyphosate as a selective agent for the production of fertile transgenic maize (Zea mays L.) plants', Molecular Breeding, vol. 10, no. 3, pp. 153-164. https://doi.org/10.1023/A:1020396708088
Howe, Arlene R. ; Gasser, Charles S. ; Brown, Sherri M. ; Padgette, Stephen R. ; Hart, Jesse ; Parker, Gregory B. ; Fromm, Michael E ; Armstrong, Charles L. / Glyphosate as a selective agent for the production of fertile transgenic maize (Zea mays L.) plants. In: Molecular Breeding. 2002 ; Vol. 10, No. 3. pp. 153-164.
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