Glycosphingolipids of cultured human colon carcinoma cells and their drug-resistant sublines

John MeKibbin, Laurie Arcolano, Karl Anders Karlsson, Goran Larson, Jan Thurin, Michael G Brattain

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human colon carcinoma cells were analyzed for lipid phosphorus, cholesterol and glycosphingolipids. Ceramide mono-, di- and trihexosides and sulfatides were isolated by column and thin-layer chromatography and determined quantitatively on the basis of their hexose content. The complex lipid fractions so isolated were only partially resolved with the material available. Gangliosides GM2 and 6M2 and globoside were major components of the fraction and were determined on the basis of their hexose, hexosamine and neuraminic acid content. The HCT 116, 116a and 116b cells contained no fucolipids. Cell lines resistant to mitomycin C, teniposide and etoposide were developed and analyzed. Over the 5 year period of the study sulfatides declined to about one-fourth of their original amounts in both parent and drug-adapted cells. HCT 116 cells adapted to mitomycin C and teniposide had 30% less ceramide monohexoside and a 45% greater cholesterol to lipid phosphorus ratio than the parent cells. Reductions in ceramide dihexoside in the drug-adapted cells were greater than those of the ceramide monohexoside. Galabiosyl ceramide was the major ceramide dihexoside in all the cells and accumulated in HCT 116a to levels 4-6-fold greater than that of the other lines as the only dihexoside.

Original languageEnglish (US)
Pages (from-to)235-246
Number of pages12
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume958
Issue number2
DOIs
StatePublished - Feb 4 1988

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Glycosphingolipids
Teniposide
Sulfoglycosphingolipids
Colon
Hexoses
Ceramides
Cells
Mitomycin
Carcinoma
Lipids
Phosphorus
Globosides
G(M2) Ganglioside
Cholesterol
Neuraminic Acids
Pharmaceutical Preparations
Hexosamines
Thin layer chromatography
Etoposide
HCT116 Cells

Keywords

  • (Human colon carcinoma cell)
  • Antitumor drug
  • Drug resistance
  • G
  • G
  • Gal α1 → 4Galβ1 → 4Glc → ceramide
  • Gal αl → 4Gal → ceramide
  • GalNAβ1 → 4[NeuAcα2 → 3]Galβ1 → 4G1 → ceramide
  • GalNacβ1 → 3Galα1 → 4Galβ1 → 4Glc → ceramide
  • Galβ1 → 3GalNAcβ1 → 4[NeuAcα2 → 3]Galβ1 → 4Glc → ceramide
  • Galβ1 → 3[Fucα1 → 4]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide
  • Galβ1 → 4Glc → ceramide
  • Galβ1 → 4GlcNAcβl → 3Galβ1 → 4Glc → ceramide
  • Galβ1 → 4[Fuc αl → 3]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide
  • Glycosphingolipid
  • HPTLC
  • Lewis a fucolipid
  • Lewis a isomer or 'X hapten'
  • NeuAc α2 → 8NeuAc α2 → 3Galβ1 → 4GlcNAcβl→ 3Galβ1→ 4Glc→ ceramide
  • NeuAca2 → 3Galβ1 → 4[Fucα1 → 3]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide
  • NeuAcα2 → 3Galβ1 → 3[Fucαl → 4]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide
  • NeuAcα2 → 3Galβ1 → 4Glc → ceramide
  • NeuAcα2 → 3Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide
  • NeuAcα2 → 8NeuAcα2 → 3Galβ1 → 4Glc → ceramide
  • TLC
  • disialosyl paragloboside
  • g
  • g
  • galabiosylceramide
  • globoside
  • globotriaosyl ceramide
  • high-perofrmance thin-layer chromatography
  • lactosyl ceramide
  • paragloboside
  • sialosyl 'X'
  • sialosyl Lewis a
  • sialosyl paragloboside
  • thin-layer chromatography

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

Cite this

Glycosphingolipids of cultured human colon carcinoma cells and their drug-resistant sublines. / MeKibbin, John; Arcolano, Laurie; Karlsson, Karl Anders; Larson, Goran; Thurin, Jan; Brattain, Michael G.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 958, No. 2, 04.02.1988, p. 235-246.

Research output: Contribution to journalArticle

MeKibbin, John ; Arcolano, Laurie ; Karlsson, Karl Anders ; Larson, Goran ; Thurin, Jan ; Brattain, Michael G. / Glycosphingolipids of cultured human colon carcinoma cells and their drug-resistant sublines. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1988 ; Vol. 958, No. 2. pp. 235-246.
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AU - MeKibbin, John

AU - Arcolano, Laurie

AU - Karlsson, Karl Anders

AU - Larson, Goran

AU - Thurin, Jan

AU - Brattain, Michael G

PY - 1988/2/4

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N2 - Human colon carcinoma cells were analyzed for lipid phosphorus, cholesterol and glycosphingolipids. Ceramide mono-, di- and trihexosides and sulfatides were isolated by column and thin-layer chromatography and determined quantitatively on the basis of their hexose content. The complex lipid fractions so isolated were only partially resolved with the material available. Gangliosides GM2 and 6M2 and globoside were major components of the fraction and were determined on the basis of their hexose, hexosamine and neuraminic acid content. The HCT 116, 116a and 116b cells contained no fucolipids. Cell lines resistant to mitomycin C, teniposide and etoposide were developed and analyzed. Over the 5 year period of the study sulfatides declined to about one-fourth of their original amounts in both parent and drug-adapted cells. HCT 116 cells adapted to mitomycin C and teniposide had 30% less ceramide monohexoside and a 45% greater cholesterol to lipid phosphorus ratio than the parent cells. Reductions in ceramide dihexoside in the drug-adapted cells were greater than those of the ceramide monohexoside. Galabiosyl ceramide was the major ceramide dihexoside in all the cells and accumulated in HCT 116a to levels 4-6-fold greater than that of the other lines as the only dihexoside.

AB - Human colon carcinoma cells were analyzed for lipid phosphorus, cholesterol and glycosphingolipids. Ceramide mono-, di- and trihexosides and sulfatides were isolated by column and thin-layer chromatography and determined quantitatively on the basis of their hexose content. The complex lipid fractions so isolated were only partially resolved with the material available. Gangliosides GM2 and 6M2 and globoside were major components of the fraction and were determined on the basis of their hexose, hexosamine and neuraminic acid content. The HCT 116, 116a and 116b cells contained no fucolipids. Cell lines resistant to mitomycin C, teniposide and etoposide were developed and analyzed. Over the 5 year period of the study sulfatides declined to about one-fourth of their original amounts in both parent and drug-adapted cells. HCT 116 cells adapted to mitomycin C and teniposide had 30% less ceramide monohexoside and a 45% greater cholesterol to lipid phosphorus ratio than the parent cells. Reductions in ceramide dihexoside in the drug-adapted cells were greater than those of the ceramide monohexoside. Galabiosyl ceramide was the major ceramide dihexoside in all the cells and accumulated in HCT 116a to levels 4-6-fold greater than that of the other lines as the only dihexoside.

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KW - Drug resistance

KW - G

KW - G

KW - Gal α1 → 4Galβ1 → 4Glc → ceramide

KW - Gal αl → 4Gal → ceramide

KW - GalNAβ1 → 4[NeuAcα2 → 3]Galβ1 → 4G1 → ceramide

KW - GalNacβ1 → 3Galα1 → 4Galβ1 → 4Glc → ceramide

KW - Galβ1 → 3GalNAcβ1 → 4[NeuAcα2 → 3]Galβ1 → 4Glc → ceramide

KW - Galβ1 → 3[Fucα1 → 4]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide

KW - Galβ1 → 4Glc → ceramide

KW - Galβ1 → 4GlcNAcβl → 3Galβ1 → 4Glc → ceramide

KW - Galβ1 → 4[Fuc αl → 3]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide

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KW - HPTLC

KW - Lewis a fucolipid

KW - Lewis a isomer or 'X hapten'

KW - NeuAc α2 → 8NeuAc α2 → 3Galβ1 → 4GlcNAcβl→ 3Galβ1→ 4Glc→ ceramide

KW - NeuAca2 → 3Galβ1 → 4[Fucα1 → 3]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide

KW - NeuAcα2 → 3Galβ1 → 3[Fucαl → 4]GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide

KW - NeuAcα2 → 3Galβ1 → 4Glc → ceramide

KW - NeuAcα2 → 3Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Glc → ceramide

KW - NeuAcα2 → 8NeuAcα2 → 3Galβ1 → 4Glc → ceramide

KW - TLC

KW - disialosyl paragloboside

KW - g

KW - g

KW - galabiosylceramide

KW - globoside

KW - globotriaosyl ceramide

KW - high-perofrmance thin-layer chromatography

KW - lactosyl ceramide

KW - paragloboside

KW - sialosyl 'X'

KW - sialosyl Lewis a

KW - sialosyl paragloboside

KW - thin-layer chromatography

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