Glycogen regulation in isolated perfused near term monkey liver

J. W. Sparks, A. Lynch, R. A. Chez, W. H. Glinsmann

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Glycogen metabolism was studied in the isolated perfused liver of the monkey conceptus at 90% of gestation using an in situ recirculating perfusion system. Net uptake of glucose and galactose and the activities of the enzymes, glycogen synthesis and phosphorylase, were studied in response to varied perfusate composition. Synthetase activity was expressed at %I, the percentage of total synthesis activity in the active form. Perfusate glucose concentrations as high as 700 mg/100 ml did not lead to net glucose uptake or to an increase in the baseline %I synthetase (4 ± 1, mean ± SEM). In the presence of 300 mg/100 ml glucose, insulin at 10-7 M increased %I to 8 ± 2, and galactose > 75 mg/100 ml increased %I to 8 ± 1. The combination of galactose, glucose, and insulin increased %I to 40 ± 5. With this latter combination, synthetase activity was proportional to perfusate glucose concentration above 100 mg/100 ml. Phosphorylase activity was diminished by either galactose or insulin, and phosphorylase activity was lowest in the group receiving galactose, glucose, and insulin. Galactose was taken up by all livers, but net glucose uptake was not observed under any condition; net hexose uptake was observed in perfusions with galactose. Glycogen levels did not very significantly with varied perfusate composition during the 30 min perfusion periods.

Original languageEnglish (US)
Pages (from-to)51-56
Number of pages6
JournalPediatric Research
Volume10
Issue number1
DOIs
StatePublished - Jan 1 1976

Fingerprint

Glycogen
Haplorhini
Galactose
Glucose
Liver
Ligases
Insulin
Phosphorylases
Perfusion
Glycogen Phosphorylase
Hexoses
Pregnancy
Enzymes

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Glycogen regulation in isolated perfused near term monkey liver. / Sparks, J. W.; Lynch, A.; Chez, R. A.; Glinsmann, W. H.

In: Pediatric Research, Vol. 10, No. 1, 01.01.1976, p. 51-56.

Research output: Contribution to journalArticle

Sparks, J. W. ; Lynch, A. ; Chez, R. A. ; Glinsmann, W. H. / Glycogen regulation in isolated perfused near term monkey liver. In: Pediatric Research. 1976 ; Vol. 10, No. 1. pp. 51-56.
@article{0852d136416740429dce917dbd290033,
title = "Glycogen regulation in isolated perfused near term monkey liver",
abstract = "Glycogen metabolism was studied in the isolated perfused liver of the monkey conceptus at 90{\%} of gestation using an in situ recirculating perfusion system. Net uptake of glucose and galactose and the activities of the enzymes, glycogen synthesis and phosphorylase, were studied in response to varied perfusate composition. Synthetase activity was expressed at {\%}I, the percentage of total synthesis activity in the active form. Perfusate glucose concentrations as high as 700 mg/100 ml did not lead to net glucose uptake or to an increase in the baseline {\%}I synthetase (4 ± 1, mean ± SEM). In the presence of 300 mg/100 ml glucose, insulin at 10-7 M increased {\%}I to 8 ± 2, and galactose > 75 mg/100 ml increased {\%}I to 8 ± 1. The combination of galactose, glucose, and insulin increased {\%}I to 40 ± 5. With this latter combination, synthetase activity was proportional to perfusate glucose concentration above 100 mg/100 ml. Phosphorylase activity was diminished by either galactose or insulin, and phosphorylase activity was lowest in the group receiving galactose, glucose, and insulin. Galactose was taken up by all livers, but net glucose uptake was not observed under any condition; net hexose uptake was observed in perfusions with galactose. Glycogen levels did not very significantly with varied perfusate composition during the 30 min perfusion periods.",
author = "Sparks, {J. W.} and A. Lynch and Chez, {R. A.} and Glinsmann, {W. H.}",
year = "1976",
month = "1",
day = "1",
doi = "10.1203/00006450-197601000-00010",
language = "English (US)",
volume = "10",
pages = "51--56",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Glycogen regulation in isolated perfused near term monkey liver

AU - Sparks, J. W.

AU - Lynch, A.

AU - Chez, R. A.

AU - Glinsmann, W. H.

PY - 1976/1/1

Y1 - 1976/1/1

N2 - Glycogen metabolism was studied in the isolated perfused liver of the monkey conceptus at 90% of gestation using an in situ recirculating perfusion system. Net uptake of glucose and galactose and the activities of the enzymes, glycogen synthesis and phosphorylase, were studied in response to varied perfusate composition. Synthetase activity was expressed at %I, the percentage of total synthesis activity in the active form. Perfusate glucose concentrations as high as 700 mg/100 ml did not lead to net glucose uptake or to an increase in the baseline %I synthetase (4 ± 1, mean ± SEM). In the presence of 300 mg/100 ml glucose, insulin at 10-7 M increased %I to 8 ± 2, and galactose > 75 mg/100 ml increased %I to 8 ± 1. The combination of galactose, glucose, and insulin increased %I to 40 ± 5. With this latter combination, synthetase activity was proportional to perfusate glucose concentration above 100 mg/100 ml. Phosphorylase activity was diminished by either galactose or insulin, and phosphorylase activity was lowest in the group receiving galactose, glucose, and insulin. Galactose was taken up by all livers, but net glucose uptake was not observed under any condition; net hexose uptake was observed in perfusions with galactose. Glycogen levels did not very significantly with varied perfusate composition during the 30 min perfusion periods.

AB - Glycogen metabolism was studied in the isolated perfused liver of the monkey conceptus at 90% of gestation using an in situ recirculating perfusion system. Net uptake of glucose and galactose and the activities of the enzymes, glycogen synthesis and phosphorylase, were studied in response to varied perfusate composition. Synthetase activity was expressed at %I, the percentage of total synthesis activity in the active form. Perfusate glucose concentrations as high as 700 mg/100 ml did not lead to net glucose uptake or to an increase in the baseline %I synthetase (4 ± 1, mean ± SEM). In the presence of 300 mg/100 ml glucose, insulin at 10-7 M increased %I to 8 ± 2, and galactose > 75 mg/100 ml increased %I to 8 ± 1. The combination of galactose, glucose, and insulin increased %I to 40 ± 5. With this latter combination, synthetase activity was proportional to perfusate glucose concentration above 100 mg/100 ml. Phosphorylase activity was diminished by either galactose or insulin, and phosphorylase activity was lowest in the group receiving galactose, glucose, and insulin. Galactose was taken up by all livers, but net glucose uptake was not observed under any condition; net hexose uptake was observed in perfusions with galactose. Glycogen levels did not very significantly with varied perfusate composition during the 30 min perfusion periods.

UR - http://www.scopus.com/inward/record.url?scp=0016892187&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016892187&partnerID=8YFLogxK

U2 - 10.1203/00006450-197601000-00010

DO - 10.1203/00006450-197601000-00010

M3 - Article

C2 - 813175

AN - SCOPUS:0016892187

VL - 10

SP - 51

EP - 56

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 1

ER -