Glycogen Metabolic Genes Are Involved in Trehalose-6-Phosphate Synthase-Mediated Regulation of Pathogenicity by the Rice Blast Fungus Magnaporthe oryzae

Muhammad Badaruddin, Lucy J. Holcombe, Richard A. Wilson, Zheng Yi Wang, Michael J. Kershaw, Nicholas J. Talbot

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The filamentous fungus Magnaporthe oryzae is the causal agent of rice blast disease. Here we show that glycogen metabolic genes play an important role in plant infection by M. oryzae. Targeted deletion of AGL1 and GPH1, which encode amyloglucosidase and glycogen phosphorylase, respectively, prevented mobilisation of glycogen stores during appressorium development and caused a significant reduction in the ability of M. oryzae to cause rice blast disease. By contrast, targeted mutation of GSN1, which encodes glycogen synthase, significantly reduced the synthesis of intracellular glycogen, but had no effect on fungal pathogenicity. We found that loss of AGL1 and GPH1 led to a reduction in expression of TPS1 and TPS3, which encode components of the trehalose-6-phosphate synthase complex, that acts as a genetic switch in M. oryzae. Tps1 responds to glucose-6-phosphate levels and the balance of NADP/NADPH to regulate virulence-associated gene expression, in association with Nmr transcriptional inhibitors. We show that deletion of the NMR3 transcriptional inhibitor gene partially restores virulence to a Δagl1Δgph1 mutant, suggesting that glycogen metabolic genes are necessary for operation of the NADPH-dependent genetic switch in M. oryzae.

Original languageEnglish (US)
Article numbere1003604
JournalPLoS pathogens
Volume9
Issue number10
DOIs
StatePublished - Oct 1 2013

Fingerprint

Magnaporthe
Glycogen
Virulence
Fungi
Genes
NADP
Glucan 1,4-alpha-Glucosidase
Glycogen Phosphorylase
Glycogen Synthase
Glucose-6-Phosphate
trehalose-6-phosphate synthase
Oryza
Gene Expression
Mutation

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Glycogen Metabolic Genes Are Involved in Trehalose-6-Phosphate Synthase-Mediated Regulation of Pathogenicity by the Rice Blast Fungus Magnaporthe oryzae. / Badaruddin, Muhammad; Holcombe, Lucy J.; Wilson, Richard A.; Wang, Zheng Yi; Kershaw, Michael J.; Talbot, Nicholas J.

In: PLoS pathogens, Vol. 9, No. 10, e1003604, 01.10.2013.

Research output: Contribution to journalArticle

Badaruddin, Muhammad ; Holcombe, Lucy J. ; Wilson, Richard A. ; Wang, Zheng Yi ; Kershaw, Michael J. ; Talbot, Nicholas J. / Glycogen Metabolic Genes Are Involved in Trehalose-6-Phosphate Synthase-Mediated Regulation of Pathogenicity by the Rice Blast Fungus Magnaporthe oryzae. In: PLoS pathogens. 2013 ; Vol. 9, No. 10.
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