Glycine turnover and oxidation and hepatic serine synthesis from glycine in fetal lambs

I. Cetin, P. V. Fennessey, A. N. Quick, A. M. Marconi, G. Meschia, F. C. Battaglia, J. W. Sparks

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

[1-13C]- and [1-14C]glycine were infused into chronically catheterized fetal lambs via a branchial vein. At tracer glycine steady state, samples were collected from the fetal abdominal aorta, umbilical vein, and fetal hepatic vein and from the maternal femoral artery and uterine vein. The samples were analyzed for plasma glycine and serine, for glycine and serine 13C atom% excess (APE), and for whole blood 14CO2 and O2 concentrations. Fetal plasma glycine disposal rate (DR) was 12.4 ± 0.8 μmol·min-1·kg fetus-1·CO2 production from decarboxylation of fetal plasma glycine was 1.63 ± 0.16 μmol·min-1·kg fetus-1 and represented 12.3 ± 0.7% of DR. Approximately 50% of infused tracer glycine was taken up by the fetal liver with the release of labeled serine and CO2 in the fetal circulation. There was no detectable efflux of tracer glycine from the placenta into the maternal circulation. The tracer production of serine and CO2 accounted for 23 and 17%, respectively, of the hepatic tracer glycine uptake. The labeled CO2 released by the liver was a large fraction (~70%) of the labeled CO2 produced by the fetus. The serine-to-glycine APE ratio in fetal plasma was ~5%. These results indicate that the fetal liver is the major site of fetal plasma glycine decarboxylation and of serine synthesis from plasma glycine.

Original languageEnglish (US)
Pages (from-to)E371-E378
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume260
Issue number3 23-3
StatePublished - Jan 1 1991

Fingerprint

Glycine
Serine
Oxidation
Liver
Plasmas
Decarboxylation
Fetus
Veins
Mothers
Umbilical Veins
Hepatic Veins
Abdominal Aorta
Femoral Artery
Placenta
Blood
Atoms

Keywords

  • Fetal hepatic glycine uptake
  • Fetal serine production
  • Placental glycine uptake
  • Stable isotopes
  • Umbilical glycine uptake

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Cetin, I., Fennessey, P. V., Quick, A. N., Marconi, A. M., Meschia, G., Battaglia, F. C., & Sparks, J. W. (1991). Glycine turnover and oxidation and hepatic serine synthesis from glycine in fetal lambs. American Journal of Physiology - Endocrinology and Metabolism, 260(3 23-3), E371-E378.

Glycine turnover and oxidation and hepatic serine synthesis from glycine in fetal lambs. / Cetin, I.; Fennessey, P. V.; Quick, A. N.; Marconi, A. M.; Meschia, G.; Battaglia, F. C.; Sparks, J. W.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 260, No. 3 23-3, 01.01.1991, p. E371-E378.

Research output: Contribution to journalArticle

Cetin, I, Fennessey, PV, Quick, AN, Marconi, AM, Meschia, G, Battaglia, FC & Sparks, JW 1991, 'Glycine turnover and oxidation and hepatic serine synthesis from glycine in fetal lambs', American Journal of Physiology - Endocrinology and Metabolism, vol. 260, no. 3 23-3, pp. E371-E378.
Cetin I, Fennessey PV, Quick AN, Marconi AM, Meschia G, Battaglia FC et al. Glycine turnover and oxidation and hepatic serine synthesis from glycine in fetal lambs. American Journal of Physiology - Endocrinology and Metabolism. 1991 Jan 1;260(3 23-3):E371-E378.
Cetin, I. ; Fennessey, P. V. ; Quick, A. N. ; Marconi, A. M. ; Meschia, G. ; Battaglia, F. C. ; Sparks, J. W. / Glycine turnover and oxidation and hepatic serine synthesis from glycine in fetal lambs. In: American Journal of Physiology - Endocrinology and Metabolism. 1991 ; Vol. 260, No. 3 23-3. pp. E371-E378.
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