Glutamate is a mediator of neurotoxicity in secretions of activated HIV-1-infected macrophages

Z. G. Jiang, C. Piggee, M. P. Heyes, C. Murphy, B. Quearry, M. Bauer, J. Zheng, H. E. Gendelman, S. p. Markey

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

We sought to identify neurotoxin(s) secreted by HIV-1-infected mononuclear phagocytes that could contribute to the pathophysiology of HIV-1-associated dementia (HAD). Neurotoxic factors were characterized in batches of conditioned media (CM) from human monocyte-derived macrophages (MDM) infected with HIV-1ADA and/or activated with lipopolysaccharide (LPS). All of the neurotoxicity was: present in the < 3000-Da fraction; blocked by 5 μM MK801; and not trypsin sensitive or extractable into polar organic solvents. Glutamate measured in CM accounted for all neurotoxic effects observed from HIV/LPS CM in astrocyte-poor neuronal cultures and may contribute to the pathophysiology of HIV-1-associated dementia.

Original languageEnglish (US)
Pages (from-to)97-107
Number of pages11
JournalJournal of Neuroimmunology
Volume117
Issue number1-2
DOIs
StatePublished - Jul 2 2001

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Keywords

  • Dementia
  • Lipopolysaccharide
  • Mononuclear phagocyte
  • NMDA
  • Neurodegeneration

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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