Glucose uptake by Listeria monocytogenes Scott A and inhibition by pediocin JD

D. P. Christensen, R. W. Hutkins

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Glucose uptake by Listeria monocytogenes Scott A was inhibited by the bacteriocin pediocin JD and by the protonophore carbonyl cyanide m- chlorophenyhydrazone. Experiments with monensin, nigericin, chlorhexidine diacetate, dinitrophenol, and gramicidin, however, showed that glucose uptake could occur in the absence of a proton motive force. L. monocytogenes cell extracts phosphorylated glucose when phosphoenolpyruvate (PEP) was present in the assay mixture, and whole cells incubated with 2-deoxyglucose accumulated 2-deoxyglucose-6-phosphate, indicating the presence of a PEP-dependent phosphotransferase system in this organism. Glucose phosphorylation also occurred when ATP was present, suggesting that a proton motive force-mediated glucose transport system may also be present. We conclude that L. monocytogenes Scott A accumulates glucose by phosphotransferase and proton motive force-mediated systems, both of which are sensitive to pediocin JD.

Original languageEnglish (US)
Pages (from-to)3870-3873
Number of pages4
JournalApplied and environmental microbiology
Volume60
Issue number10
StatePublished - Jan 1 1994

Fingerprint

pediocins
Listeria monocytogenes
glucose
Glucose
Proton-Motive Force
2-deoxyglucose
Phosphoenolpyruvate
phosphotransferases (kinases)
Phosphotransferases
gramicidin
nigericin
Nigericin
Dinitrophenols
chlorhexidine
Gramicidin
Monensin
Bacteriocins
Chlorhexidine
monensin
Deoxyglucose

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Glucose uptake by Listeria monocytogenes Scott A and inhibition by pediocin JD. / Christensen, D. P.; Hutkins, R. W.

In: Applied and environmental microbiology, Vol. 60, No. 10, 01.01.1994, p. 3870-3873.

Research output: Contribution to journalArticle

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