Glucose Metabolism and AMPK Signaling Regulate Dopaminergic Cell Death Induced by Gene (α-Synuclein)-Environment (Paraquat) Interactions

Annadurai Anandhan, Shulei Lei, Roman Levytskyy, Aglaia Pappa, Mihalis I. Panayiotidis, Ronald Cerny, Oleh Khalimonchuk, Robert Powers, Rodrigo Franco-Cruz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

While environmental exposures are not the single cause of Parkinson’s disease (PD), their interaction with genetic alterations is thought to contribute to neuronal dopaminergic degeneration. However, the mechanisms involved in dopaminergic cell death induced by gene-environment interactions remain unclear. In this work, we have revealed for the first time the role of central carbon metabolism and metabolic dysfunction in dopaminergic cell death induced by the paraquat (PQ)-α-synuclein interaction. The toxicity of PQ in dopaminergic N27 cells was significantly reduced by glucose deprivation, inhibition of hexokinase with 2-deoxy-D-glucose (2-DG), or equimolar substitution of glucose with galactose, which evidenced the contribution of glucose metabolism to PQ-induced cell death. PQ also stimulated an increase in glucose uptake, and in the levels of glucose transporter type 4 (GLUT4) and Na+-glucose transporters isoform 1 (SGLT1) proteins, but only inhibition of GLUT-like transport with STF-31 or ascorbic acid reduced PQ-induced cell death. Importantly, while autophagy protein 5 (ATG5)/unc-51 like autophagy activating kinase 1 (ULK1)-dependent autophagy protected against PQ toxicity, the inhibitory effect of glucose deprivation on cell death progression was largely independent of autophagy or mammalian target of rapamycin (mTOR) signaling. PQ selectively induced metabolomic alterations and adenosine monophosphate-activated protein kinase (AMPK) activation in the midbrain and striatum of mice chronically treated with PQ. Inhibition of AMPK signaling led to metabolic dysfunction and an enhanced sensitivity of dopaminergic cells to PQ. In addition, activation of AMPK by PQ was prevented by inhibition of the inducible nitric oxide syntase (iNOS) with 1400W, but PQ had no effect on iNOS levels. Overexpression of wild type or A53T mutant α-synuclein stimulated glucose accumulation and PQ toxicity, and this toxic synergism was reduced by inhibition of glucose metabolism/transport and the pentose phosphate pathway (6-aminonicotinamide). These results demonstrate that glucose metabolism and AMPK regulate dopaminergic cell death induced by gene (α-synuclein)-environment (PQ) interactions.

Original languageEnglish (US)
Pages (from-to)3825-3842
Number of pages18
JournalMolecular Neurobiology
Volume54
Issue number5
DOIs
StatePublished - Jul 1 2017

Fingerprint

Synucleins
Paraquat
Adenosine Monophosphate
Protein Kinases
Cell Death
Glucose
Genes
Autophagy
Sodium-Glucose Transporter 1
Nitric Oxide
6-Aminonicotinamide
Glucose Transporter Type 4
Pentose Phosphate Pathway
Gene-Environment Interaction
Hexokinase
Metabolomics
Facilitative Glucose Transport Proteins
Poisons
Environmental Exposure
Deoxyglucose

Keywords

  • Adenosine monophosphate-activated kinase
  • Autophagy
  • Glucose transporters
  • Glycolysis
  • Metabolomics
  • Parkinson’s disease
  • Pesticides
  • α-Synuclein

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Glucose Metabolism and AMPK Signaling Regulate Dopaminergic Cell Death Induced by Gene (α-Synuclein)-Environment (Paraquat) Interactions. / Anandhan, Annadurai; Lei, Shulei; Levytskyy, Roman; Pappa, Aglaia; Panayiotidis, Mihalis I.; Cerny, Ronald; Khalimonchuk, Oleh; Powers, Robert; Franco-Cruz, Rodrigo.

In: Molecular Neurobiology, Vol. 54, No. 5, 01.07.2017, p. 3825-3842.

Research output: Contribution to journalArticle

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