Glucose-ABL1-TOR Signaling Modulates Cell Cycle Tuning to Control Terminal Appressorial Cell Differentiation

Margarita Marroquin-Guzman, Guangchao Sun, Richard A. Wilson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The conserved target of rapamycin (TOR) pathway integrates growth and development with available nutrients, but how cellular glucose controls TOR function and signaling is poorly understood. Here, we provide functional evidence from the devastating rice blast fungus Magnaporthe oryzae that glucose can mediate TOR activity via the product of a novel carbon-responsive gene, ABL1, in order to tune cell cycle progression during infection-related development. Under nutrient-free conditions, wild type (WT) M. oryzae strains form terminal plant-infecting cells (appressoria) at the tips of germ tubes emerging from three-celled spores (conidia). WT appressorial development is accompanied by one round of mitosis followed by autophagic cell death of the conidium. In contrast, Δabl1 mutant strains undergo multiple rounds of accelerated mitosis in elongated germ tubes, produce few appressoria, and are abolished for autophagy. Treating WT spores with glucose or 2-deoxyglucose phenocopied Δabl1. Inactivating TOR in Δabl1 mutants or glucose-treated WT strains restored appressorium formation by promoting mitotic arrest at G1/G0 via an appressorium- and autophagy-inducing cell cycle delay at G2/M. Collectively, this work uncovers a novel glucose-ABL1-TOR signaling axis and shows it engages two metabolic checkpoints in order to modulate cell cycle tuning and mediate terminal appressorial cell differentiation. We thus provide new molecular insights into TOR regulation and cell development in response to glucose.

Original languageEnglish (US)
Article numbere1006557
JournalPLoS genetics
Volume13
Issue number1
DOIs
StatePublished - Jan 2017

Fingerprint

Sirolimus
cell differentiation
Cell Differentiation
cell cycle
Cell Cycle
glucose
appressoria
Glucose
autophagy
Autophagy
Magnaporthe oryzae
Fungal Spores
germ tube
Spores
Mitosis
mitosis
conidia
spore
spores
Magnaporthe

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Glucose-ABL1-TOR Signaling Modulates Cell Cycle Tuning to Control Terminal Appressorial Cell Differentiation. / Marroquin-Guzman, Margarita; Sun, Guangchao; Wilson, Richard A.

In: PLoS genetics, Vol. 13, No. 1, e1006557, 01.2017.

Research output: Contribution to journalArticle

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