GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDTRANSFERASE1 (GINT1) is a GlcNAc-containing glycosylinositol phosphorylceramide glycosyltransferase

Toshiki Ishikawa, Lin Fang, Emilie A. Rennie, Julien Sechet, Jingwei Yan, Beibei Jing, William Moore, Edgar B Cahoon, Henrik V. Scheller, Maki Kawai-Yamada, Jenny C. Mortimer

Research output: Contribution to journalArticle

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Abstract

Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane. Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability. However, the function of GIPCs and the significance of their structural variation are poorly understood. Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)]. In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants. However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal. Furthermore, we could produce, de novo, “rice-like” GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup. This study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function. We also identify a possible role for GIPCs in maintaining cell-cell adhesion.

Original languageEnglish (US)
Pages (from-to)938-952
Number of pages15
JournalPlant Physiology
Volume177
Issue number3
DOIs
StatePublished - Jun 1 2018

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Glycosyltransferases
glycosyltransferases
glucosamine
Glucosamine
Arabidopsis
glycosylation
Glycosylation
polysaccharides
Polysaccharides
Seeds
rice
sphingolipids
ceramides
Sphingolipids
Liliopsida
lethal genes
cell adhesion
Ceramides
abiotic stress
vegetative growth

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDTRANSFERASE1 (GINT1) is a GlcNAc-containing glycosylinositol phosphorylceramide glycosyltransferase. / Ishikawa, Toshiki; Fang, Lin; Rennie, Emilie A.; Sechet, Julien; Yan, Jingwei; Jing, Beibei; Moore, William; Cahoon, Edgar B; Scheller, Henrik V.; Kawai-Yamada, Maki; Mortimer, Jenny C.

In: Plant Physiology, Vol. 177, No. 3, 01.06.2018, p. 938-952.

Research output: Contribution to journalArticle

Ishikawa, T, Fang, L, Rennie, EA, Sechet, J, Yan, J, Jing, B, Moore, W, Cahoon, EB, Scheller, HV, Kawai-Yamada, M & Mortimer, JC 2018, 'GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDTRANSFERASE1 (GINT1) is a GlcNAc-containing glycosylinositol phosphorylceramide glycosyltransferase', Plant Physiology, vol. 177, no. 3, pp. 938-952. https://doi.org/10.1104/pp.18.00396
Ishikawa, Toshiki ; Fang, Lin ; Rennie, Emilie A. ; Sechet, Julien ; Yan, Jingwei ; Jing, Beibei ; Moore, William ; Cahoon, Edgar B ; Scheller, Henrik V. ; Kawai-Yamada, Maki ; Mortimer, Jenny C. / GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDTRANSFERASE1 (GINT1) is a GlcNAc-containing glycosylinositol phosphorylceramide glycosyltransferase. In: Plant Physiology. 2018 ; Vol. 177, No. 3. pp. 938-952.
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AU - Sechet, Julien

AU - Yan, Jingwei

AU - Jing, Beibei

AU - Moore, William

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AU - Scheller, Henrik V.

AU - Kawai-Yamada, Maki

AU - Mortimer, Jenny C.

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