Global transcriptome analysis of staphylococcus aureus biofilms in response to innate immune cells

Tyler D. Scherr, Christelle M. Roux, Mark L. Hanke, Amanda Angle, Paul M. Dunman, Tammy L Kielian

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The potent phagocytic and microbicidal of neutrophils and macrophages are among the first lines of defense against bacterial infections. Yet Staphylococcus aureus is often resistant to innate immune defense mechanisms, especially when organized as a biofilm. To investigate how S. aureus biofilms respond to macrophages and neutrophils, gene expression patterns were profiled using Affymetrix microarrays. The addition of macrophages to S. aureus static biofilms led to a global suppression of the biofilm transcriptome with a wide variety of genes downregulated. Notably, genes involved in metabolism, cell wall synthesis/structure, and transcription/translation/replication were among the most highly downregulated, which was most dramatic at 1 h compared to 24 h following macrophage addition to biofilms. Unexpectedly, few genes were enhanced in biofilms after macrophage challenge. Unlike coculture with macrophages, coculture of S. aureus static biofilms with neutrophils did not greatly influence the biofilm transcriptome. Collectively, these experiments demonstrate that S. aureus biofilms differentially modify their gene expression patterns depending on the leukocyte subset encountered.

Original languageEnglish (US)
Pages (from-to)4363-4376
Number of pages14
JournalInfection and immunity
Volume81
Issue number12
DOIs
StatePublished - Dec 1 2013

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Gene Expression Profiling
Biofilms
Innate Immunity
Staphylococcus aureus
Macrophages
Neutrophils
Coculture Techniques
Transcriptome
Down-Regulation
Genes
Gene Expression
Bacterial Infections
Cell Wall
Leukocytes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Global transcriptome analysis of staphylococcus aureus biofilms in response to innate immune cells. / Scherr, Tyler D.; Roux, Christelle M.; Hanke, Mark L.; Angle, Amanda; Dunman, Paul M.; Kielian, Tammy L.

In: Infection and immunity, Vol. 81, No. 12, 01.12.2013, p. 4363-4376.

Research output: Contribution to journalArticle

Scherr, Tyler D. ; Roux, Christelle M. ; Hanke, Mark L. ; Angle, Amanda ; Dunman, Paul M. ; Kielian, Tammy L. / Global transcriptome analysis of staphylococcus aureus biofilms in response to innate immune cells. In: Infection and immunity. 2013 ; Vol. 81, No. 12. pp. 4363-4376.
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