Global proteomic analysis reveals an exclusive role of thylakoid membranes in bioenergetics of a model cyanobacterium

Michelle Liberton, Rajib Saha, Jon M. Jacobs, Amelia Y. Nguyen, Marina A. Gritsenko, Richard D. Smith, David W. Koppenaal, Himadri B. Pakrasi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cyanobacteria are photosynthetic microbes with highly differentiated membrane systems. These organisms contain an outer membrane, plasma membrane, and an internal system of thylakoid membranes where the photosynthetic and respiratory machinery are found. This existence of compartmentalization and differentiation of membrane systems poses a number of challenges for cyanobacterial cells in terms of organization and distribution of proteins to the correct membrane system. Proteomics studies have long sought to identify the components of the different membrane systems in cyanobacteria, and to date about 450 different proteins have been attributed to either the plasma membrane or thylakoid membrane. Given the complexity of these membranes, many more proteins remain to be identified, and a comprehensive catalogue of plasma membrane and thylakoid membrane proteins is needed. Here we describe the identification of 635 differentially localized proteins in Synechocystis sp. PCC 6803 by quantitative iTRAQ isobaric labeling; of these, 459 proteins were localized to the plasma membrane and 176 were localized to the thylakoid membrane. Surprisingly, we found over 2.5 times the number of unique proteins identified in the plasma membrane compared with the thylakoid membrane. This suggests that the protein composition of the thylakoid membrane is more homogeneous than the plasma membrane, consistent with the role of the plasma membrane in diverse cellular processes including protein trafficking and nutrient import, compared with a more specialized role for the thylakoid membrane in cellular energetics. Thus, our data clearly define the two membrane systems with distinct functions. Over-all, the protein compositions of the Synechocystis 6803 plasma membrane and thylakoid membrane are quite similar to that of the plasma membrane of Escherichia coli and thylakoid membrane of Arabidopsis chloroplasts, respectively. Synechocystis 6803 can therefore be described as a Gram-negative bacterium with an additional internal membrane system that fulfills the energetic requirements of the cell.

Original languageEnglish (US)
Pages (from-to)2021-2032
Number of pages12
JournalMolecular and Cellular Proteomics
Volume15
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

Thylakoids
Cyanobacteria
Proteomics
Energy Metabolism
Cell Membrane
Membranes
Cell membranes
Synechocystis
Thylakoid Membrane Proteins
Proteins
Protein Transport
Chloroplasts
Gram-Negative Bacteria
Arabidopsis
Escherichia coli
Food
Chemical analysis
Labeling
Nutrients

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Cite this

Global proteomic analysis reveals an exclusive role of thylakoid membranes in bioenergetics of a model cyanobacterium. / Liberton, Michelle; Saha, Rajib; Jacobs, Jon M.; Nguyen, Amelia Y.; Gritsenko, Marina A.; Smith, Richard D.; Koppenaal, David W.; Pakrasi, Himadri B.

In: Molecular and Cellular Proteomics, Vol. 15, No. 6, 01.06.2016, p. 2021-2032.

Research output: Contribution to journalArticle

Liberton, M, Saha, R, Jacobs, JM, Nguyen, AY, Gritsenko, MA, Smith, RD, Koppenaal, DW & Pakrasi, HB 2016, 'Global proteomic analysis reveals an exclusive role of thylakoid membranes in bioenergetics of a model cyanobacterium', Molecular and Cellular Proteomics, vol. 15, no. 6, pp. 2021-2032. https://doi.org/10.1074/mcp.M115.057240
Liberton, Michelle ; Saha, Rajib ; Jacobs, Jon M. ; Nguyen, Amelia Y. ; Gritsenko, Marina A. ; Smith, Richard D. ; Koppenaal, David W. ; Pakrasi, Himadri B. / Global proteomic analysis reveals an exclusive role of thylakoid membranes in bioenergetics of a model cyanobacterium. In: Molecular and Cellular Proteomics. 2016 ; Vol. 15, No. 6. pp. 2021-2032.
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