Global changes in mineral transporters in tetraploid switchgrasses (Panicum virgatum L.)

Nathan A. Palmer, Aaron J. Saathoff, Brian M. Waters, Teresa Donze, Tiffany M. Heng-Moss, Paul Twigg, Christian M. Tobias, Gautam Sarath

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Switchgrass (Panicum virgatum L) is perennial, C4 grass with great potential as a biofuel crop. An in-depth understanding of the mechanisms that control mineral uptake, distribution and remobilization will benefit sustainable production. Nutrients are mobilized from aerial portions to below-ground crowns and rhizomes as a natural accompaniment to above-ground senescence post seed-set. Mineral uptake and remobilization is dependent on transporters, however, little if any information is available about the specific transporters that are needed and how their relative expression changes over a growing season. Using well-defined classes of mineral transporters, we identified 520 genes belonging to 40 different transporter classes in the tetraploid switchgrass genome. Expression patterns were determined for many of these genes using publically available transcriptomic datasets obtained from both greenhouse and field grown plants. Certain transporters showed strong temporal patterns of expression in distinct developmental stages of the plant. Gene-expression was verified for selected transporters using qRT-PCR. By and large these analyses confirmed the developmental stage-specific expression of these genes. Mineral analyses indicated that K, Fe, Mg, Co, and As had a similar pattern of accumulation with apparent limited remobilization at the end of the growing season. These initial analyses will serve as a foundation for more detailed examination of the nutrient biology of switchgrass.

Original languageEnglish (US)
Article number549
JournalFrontiers in Plant Science
Volume4
Issue numberJAN
DOIs
StatePublished - Jan 2 2014

Fingerprint

Panicum virgatum
global change
tetraploidy
transporters
minerals
growing season
developmental stages
uptake mechanisms
gene expression
root crown
energy crops
nutrients
seed set
transcriptomics
rhizomes
genes
grasses
greenhouses
Biological Sciences
genome

Keywords

  • Bioenergy
  • Crowns and rhizomes
  • Growing-season
  • Mineral transporters
  • Nutrients
  • Panicum virgatum
  • Switchgrass
  • qPCR

ASJC Scopus subject areas

  • Plant Science

Cite this

Palmer, N. A., Saathoff, A. J., Waters, B. M., Donze, T., Heng-Moss, T. M., Twigg, P., ... Sarath, G. (2014). Global changes in mineral transporters in tetraploid switchgrasses (Panicum virgatum L.). Frontiers in Plant Science, 4(JAN), [549]. https://doi.org/10.3389/fpls.2013.00549

Global changes in mineral transporters in tetraploid switchgrasses (Panicum virgatum L.). / Palmer, Nathan A.; Saathoff, Aaron J.; Waters, Brian M.; Donze, Teresa; Heng-Moss, Tiffany M.; Twigg, Paul; Tobias, Christian M.; Sarath, Gautam.

In: Frontiers in Plant Science, Vol. 4, No. JAN, 549, 02.01.2014.

Research output: Contribution to journalArticle

Palmer, NA, Saathoff, AJ, Waters, BM, Donze, T, Heng-Moss, TM, Twigg, P, Tobias, CM & Sarath, G 2014, 'Global changes in mineral transporters in tetraploid switchgrasses (Panicum virgatum L.)', Frontiers in Plant Science, vol. 4, no. JAN, 549. https://doi.org/10.3389/fpls.2013.00549
Palmer, Nathan A. ; Saathoff, Aaron J. ; Waters, Brian M. ; Donze, Teresa ; Heng-Moss, Tiffany M. ; Twigg, Paul ; Tobias, Christian M. ; Sarath, Gautam. / Global changes in mineral transporters in tetraploid switchgrasses (Panicum virgatum L.). In: Frontiers in Plant Science. 2014 ; Vol. 4, No. JAN.
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