Giardia spp.

Distribution of contractile proteins in the attachment organelle

Dennis E Feely, Judy V. Schollmeyer, Stanley L. Erlandsen

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Giardia spp. trophozoites isolated from rat small intestine were examined by light microscopy, electron microscopy, SDS-gel electrophoresis, and immunocytochemistry. In SDS-gels of protein extracts of isolated Giardia spp. trophozoites protein bands corresponding to myosin, α-actinin, and actin were identified by comigration with avian myofibril proteins and molecular weight standards. Actin was specifically identified in SDS-gels by immunoautoradiography. Immunostaining for actin, α-actinin, myosin, and tropomyosin in trophozoites was demonstrated in the periphery of the ventral disc in an area corresponding to the lateral crest. Electron-dense fibrillar was observed in the lateral crest of the ventral disc by electron microscopy. Immunostaining for actin and α-actinin was also observed in the area of the median body, a microtubular organelle, and in electron-dense fibrillar material associated with the intracellular axonemes of the posterior-lateral flagella. The localization of these contractile proteins in the ventral disc suggests that they may play an important role in the mechanism of trophozoite attachment.

Original languageEnglish (US)
Pages (from-to)145-154
Number of pages10
JournalExperimental Parasitology
Volume53
Issue number1
DOIs
StatePublished - Jan 1 1982

Fingerprint

Giardia
Contractile Proteins
Trophozoites
Actinin
Organelles
Actins
Gels
Myosins
Electron Microscopy
Avian Proteins
Electrons
Axoneme
Tropomyosin
Flagella
Myofibrils
Small Intestine
Electrophoresis
Microscopy
Proteins
Molecular Weight

Keywords

  • Attachment organelle
  • Biochemistry
  • Giardia duodenalis
  • Giardia muris
  • Immunochemistry
  • Morphology
  • Proteins, contractile
  • Protozoa, parasitic

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

Cite this

Giardia spp. Distribution of contractile proteins in the attachment organelle. / Feely, Dennis E; Schollmeyer, Judy V.; Erlandsen, Stanley L.

In: Experimental Parasitology, Vol. 53, No. 1, 01.01.1982, p. 145-154.

Research output: Contribution to journalArticle

Feely, Dennis E ; Schollmeyer, Judy V. ; Erlandsen, Stanley L. / Giardia spp. Distribution of contractile proteins in the attachment organelle. In: Experimental Parasitology. 1982 ; Vol. 53, No. 1. pp. 145-154.
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