Gentle lysis of mucous producing cold-adapted bacteria by surfactant treatment combined with mechanical disruption

Renu Nandakumar, A. M. Gounot, B. Mattiasson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A procedure for the enhanced lysis of mucous producing psychrotrophic Gram positive bacteria for subsequent enzyme studies is described. An initial washing of bacterial cells with Tween 80 was found to improve the degree of cell disruption in subsequent sonication or grinding with glass beads, resulting in about 20-200% increase in total soluble protein content. However, in terms of lactate dehydrogenase (LDH) activity present in the lysate, pretreatment with Tween 80 was more effective in combination with grinding, especially in the highly mucous producing strain GY11. The type of surfactant used in the pretreatment procedure before grinding strongly influenced the percentage lysis of tested strains, both in terms of released soluble protein and enzyme activity. Zymograms of LDH and glutamate dehydrogenase (GDH) activity present in the lysates also very well supported the results obtained by total protein and enzyme activity measurements. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalJournal of Biotechnology
Volume83
Issue number3
DOIs
StatePublished - Oct 13 2000

Fingerprint

Surface-Active Agents
Bacteria
Surface active agents
Polysorbates
Enzyme activity
Proteins
L-Lactate Dehydrogenase
Enzymes
Glutamate Dehydrogenase
Sonication
Gram-Positive Bacteria
Washing
Glass
Oxidoreductases

Keywords

  • Cell lysis
  • Psychrotrophic bacteria
  • Surfactants

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Gentle lysis of mucous producing cold-adapted bacteria by surfactant treatment combined with mechanical disruption. / Nandakumar, Renu; Gounot, A. M.; Mattiasson, B.

In: Journal of Biotechnology, Vol. 83, No. 3, 13.10.2000, p. 211-217.

Research output: Contribution to journalArticle

Nandakumar, Renu ; Gounot, A. M. ; Mattiasson, B. / Gentle lysis of mucous producing cold-adapted bacteria by surfactant treatment combined with mechanical disruption. In: Journal of Biotechnology. 2000 ; Vol. 83, No. 3. pp. 211-217.
@article{b297a064e37b4936ac29bdb87b3459ea,
title = "Gentle lysis of mucous producing cold-adapted bacteria by surfactant treatment combined with mechanical disruption",
abstract = "A procedure for the enhanced lysis of mucous producing psychrotrophic Gram positive bacteria for subsequent enzyme studies is described. An initial washing of bacterial cells with Tween 80 was found to improve the degree of cell disruption in subsequent sonication or grinding with glass beads, resulting in about 20-200{\%} increase in total soluble protein content. However, in terms of lactate dehydrogenase (LDH) activity present in the lysate, pretreatment with Tween 80 was more effective in combination with grinding, especially in the highly mucous producing strain GY11. The type of surfactant used in the pretreatment procedure before grinding strongly influenced the percentage lysis of tested strains, both in terms of released soluble protein and enzyme activity. Zymograms of LDH and glutamate dehydrogenase (GDH) activity present in the lysates also very well supported the results obtained by total protein and enzyme activity measurements. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "Cell lysis, Psychrotrophic bacteria, Surfactants",
author = "Renu Nandakumar and Gounot, {A. M.} and B. Mattiasson",
year = "2000",
month = "10",
day = "13",
doi = "10.1016/S0168-1656(00)00317-5",
language = "English (US)",
volume = "83",
pages = "211--217",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Gentle lysis of mucous producing cold-adapted bacteria by surfactant treatment combined with mechanical disruption

AU - Nandakumar, Renu

AU - Gounot, A. M.

AU - Mattiasson, B.

PY - 2000/10/13

Y1 - 2000/10/13

N2 - A procedure for the enhanced lysis of mucous producing psychrotrophic Gram positive bacteria for subsequent enzyme studies is described. An initial washing of bacterial cells with Tween 80 was found to improve the degree of cell disruption in subsequent sonication or grinding with glass beads, resulting in about 20-200% increase in total soluble protein content. However, in terms of lactate dehydrogenase (LDH) activity present in the lysate, pretreatment with Tween 80 was more effective in combination with grinding, especially in the highly mucous producing strain GY11. The type of surfactant used in the pretreatment procedure before grinding strongly influenced the percentage lysis of tested strains, both in terms of released soluble protein and enzyme activity. Zymograms of LDH and glutamate dehydrogenase (GDH) activity present in the lysates also very well supported the results obtained by total protein and enzyme activity measurements. Copyright (C) 2000 Elsevier Science B.V.

AB - A procedure for the enhanced lysis of mucous producing psychrotrophic Gram positive bacteria for subsequent enzyme studies is described. An initial washing of bacterial cells with Tween 80 was found to improve the degree of cell disruption in subsequent sonication or grinding with glass beads, resulting in about 20-200% increase in total soluble protein content. However, in terms of lactate dehydrogenase (LDH) activity present in the lysate, pretreatment with Tween 80 was more effective in combination with grinding, especially in the highly mucous producing strain GY11. The type of surfactant used in the pretreatment procedure before grinding strongly influenced the percentage lysis of tested strains, both in terms of released soluble protein and enzyme activity. Zymograms of LDH and glutamate dehydrogenase (GDH) activity present in the lysates also very well supported the results obtained by total protein and enzyme activity measurements. Copyright (C) 2000 Elsevier Science B.V.

KW - Cell lysis

KW - Psychrotrophic bacteria

KW - Surfactants

UR - http://www.scopus.com/inward/record.url?scp=0034644551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034644551&partnerID=8YFLogxK

U2 - 10.1016/S0168-1656(00)00317-5

DO - 10.1016/S0168-1656(00)00317-5

M3 - Article

VL - 83

SP - 211

EP - 217

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

IS - 3

ER -