Genotypic diversity and mixed infection in newborn disease and hearing loss in congenital cytomegalovirus infection

Sunil K. Pati, Swetha Pinninti, Zdenek Novak, Nazma Chowdhury, Raj K. Patro, Karen Fowler, Shannon Ross, Suresh Boppana

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: Congenital cytomegalovirus (cCMV) is a common congenital infection and a leading nongenetic cause of sensorineural hearing loss (SNHL). CMV exhibits extensive genetic variability, and infection with multiple CMV strains (mixed infection) was shown to be common in congenital CMV. The role of mixed infections in disease and outcome remains to be defined. Methods: Genotyping of envelope glycoproteins, UL55 (gB), UL73 (gN) and UL75 (gH), was performed on saliva specimens of 79 infants from the ongoing CMV and Hearing Multicenter Screening (CHIMES) Study and on blood and urine specimens of 52 infants who participated in natural history studies at the University of Alabama at Birmingham. Genotyping of UL144 and US28 was also performed in the CHIMES cohort. The association of individual genotypes and mixed infection with clinical findings at birth and SNHL was examined. Results: Thirty-seven of 131 infants (28%) were symptomatic at birth and 26 (20%) had SNHL at birth. All known genotypes of UL55, UL75, UL73 and US28 were represented, and no particular genotype was associated with symptomatic infection or SNHL. UL144 subtype C was more common in symptomatic infants but not associated with SNHL. Mixed infection was observed in 59 infants (45%) and not associated with symptoms (P = 0.43) or SNHL at birth (P = 0.82). In the cohort of 52 infants with long-term hearing outcome, mixed infection at birth was not predictive of SNHL. Conclusions: Mixed infection is common in infants with congenital CMV but is neither associated with symptomatic infection nor associated with SNHL.

Original languageEnglish (US)
Pages (from-to)1050-1054
Number of pages5
JournalPediatric Infectious Disease Journal
Volume32
Issue number10
DOIs
StatePublished - May 23 2013

Fingerprint

Sensorineural Hearing Loss
Cytomegalovirus Infections
Coinfection
Hearing Loss
Newborn Infant
Parturition
Hearing
Genotype
Infection
Natural History
Cytomegalovirus
Saliva
Multicenter Studies
Glycoproteins
Urine

Keywords

  • Congenital infection
  • Cytomegalovirus
  • Genotypes
  • Hearing loss
  • Mixed infection

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Genotypic diversity and mixed infection in newborn disease and hearing loss in congenital cytomegalovirus infection. / Pati, Sunil K.; Pinninti, Swetha; Novak, Zdenek; Chowdhury, Nazma; Patro, Raj K.; Fowler, Karen; Ross, Shannon; Boppana, Suresh.

In: Pediatric Infectious Disease Journal, Vol. 32, No. 10, 23.05.2013, p. 1050-1054.

Research output: Contribution to journalArticle

Pati, SK, Pinninti, S, Novak, Z, Chowdhury, N, Patro, RK, Fowler, K, Ross, S & Boppana, S 2013, 'Genotypic diversity and mixed infection in newborn disease and hearing loss in congenital cytomegalovirus infection', Pediatric Infectious Disease Journal, vol. 32, no. 10, pp. 1050-1054. https://doi.org/10.1097/INF.0b013e31829bb0b9
Pati, Sunil K. ; Pinninti, Swetha ; Novak, Zdenek ; Chowdhury, Nazma ; Patro, Raj K. ; Fowler, Karen ; Ross, Shannon ; Boppana, Suresh. / Genotypic diversity and mixed infection in newborn disease and hearing loss in congenital cytomegalovirus infection. In: Pediatric Infectious Disease Journal. 2013 ; Vol. 32, No. 10. pp. 1050-1054.
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AU - Pinninti, Swetha

AU - Novak, Zdenek

AU - Chowdhury, Nazma

AU - Patro, Raj K.

AU - Fowler, Karen

AU - Ross, Shannon

AU - Boppana, Suresh

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N2 - Background: Congenital cytomegalovirus (cCMV) is a common congenital infection and a leading nongenetic cause of sensorineural hearing loss (SNHL). CMV exhibits extensive genetic variability, and infection with multiple CMV strains (mixed infection) was shown to be common in congenital CMV. The role of mixed infections in disease and outcome remains to be defined. Methods: Genotyping of envelope glycoproteins, UL55 (gB), UL73 (gN) and UL75 (gH), was performed on saliva specimens of 79 infants from the ongoing CMV and Hearing Multicenter Screening (CHIMES) Study and on blood and urine specimens of 52 infants who participated in natural history studies at the University of Alabama at Birmingham. Genotyping of UL144 and US28 was also performed in the CHIMES cohort. The association of individual genotypes and mixed infection with clinical findings at birth and SNHL was examined. Results: Thirty-seven of 131 infants (28%) were symptomatic at birth and 26 (20%) had SNHL at birth. All known genotypes of UL55, UL75, UL73 and US28 were represented, and no particular genotype was associated with symptomatic infection or SNHL. UL144 subtype C was more common in symptomatic infants but not associated with SNHL. Mixed infection was observed in 59 infants (45%) and not associated with symptoms (P = 0.43) or SNHL at birth (P = 0.82). In the cohort of 52 infants with long-term hearing outcome, mixed infection at birth was not predictive of SNHL. Conclusions: Mixed infection is common in infants with congenital CMV but is neither associated with symptomatic infection nor associated with SNHL.

AB - Background: Congenital cytomegalovirus (cCMV) is a common congenital infection and a leading nongenetic cause of sensorineural hearing loss (SNHL). CMV exhibits extensive genetic variability, and infection with multiple CMV strains (mixed infection) was shown to be common in congenital CMV. The role of mixed infections in disease and outcome remains to be defined. Methods: Genotyping of envelope glycoproteins, UL55 (gB), UL73 (gN) and UL75 (gH), was performed on saliva specimens of 79 infants from the ongoing CMV and Hearing Multicenter Screening (CHIMES) Study and on blood and urine specimens of 52 infants who participated in natural history studies at the University of Alabama at Birmingham. Genotyping of UL144 and US28 was also performed in the CHIMES cohort. The association of individual genotypes and mixed infection with clinical findings at birth and SNHL was examined. Results: Thirty-seven of 131 infants (28%) were symptomatic at birth and 26 (20%) had SNHL at birth. All known genotypes of UL55, UL75, UL73 and US28 were represented, and no particular genotype was associated with symptomatic infection or SNHL. UL144 subtype C was more common in symptomatic infants but not associated with SNHL. Mixed infection was observed in 59 infants (45%) and not associated with symptoms (P = 0.43) or SNHL at birth (P = 0.82). In the cohort of 52 infants with long-term hearing outcome, mixed infection at birth was not predictive of SNHL. Conclusions: Mixed infection is common in infants with congenital CMV but is neither associated with symptomatic infection nor associated with SNHL.

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