Genotypic characterization and prevalence of virulence factors among Canadian Escherichia coli O157:H7 strains

Kim Ziebell, Marina Steele, Yongxiang Zhang, Andrew Benson, Eduardo N. Taboada, Chad Laing, Scott McEwen, Bruce Ciebin, Roger Johnson, Victor Gannon

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In this study, the association between genotypic and selected phenotypic characteristics was examined in a collection of Canadian Escherichia coli O157:H7 strains isolated from humans and cattle in the provinces of Alberta, Ontario, Saskatchewan, and Quebec. In a subset of 69 strains selected on the basis of specific phage types (PTs), a strong correlation between the lineage-specific polymorphism assay (LSPA6) genotype and PT was observed with all strains of PTs 4, 14, 21, 31, 33, and 87 belonging to the LSPA6 lineage I (LSPA6-LI) genotype, while those of PTs 23, 45, 67, and 74 belonged to LSPA6 lineage II (LSPA6-LII) genotypes. This correlation was maintained when additional strains of each PT were tested. E. coli O157:H7 strains with the LSPA6-LI genotype were much more common in the collection than were the LSPA6-LII or lineage I/II (LSPA6-LI/II)-related genotypes (82.6, 11.2, and 5.8%, respectively). Of the strains tested, proportionately more LSPA6-LI than LSPA6-LII genotype strains were isolated from humans (52.7% versus 19.7%) than from cattle (47.8% versus 80.2%). In addition, 96.7% of the LSPA6-LII strains carried the stx2c variant gene, while only 50.0% of LSPA6-LI/II and 2.7% of LSPA6-LI strains carried this gene. LSPA6-LII strains were also significantly more likely to possess the colicin D gene, cda (50.8% versus 23.2%), and have combined resistance to streptomycin, sulfisoxazole, and tetracycline (72.1% versus 0.9%) than were LSPA6-LI strains. The LSPA6 genotype- and PT-related characteristics identified may be important markers of specific ecotypes of E. coli O157:H7 that have unique epidemiological and virulence characteristics.

Original languageEnglish (US)
Pages (from-to)4314-4323
Number of pages10
JournalApplied and environmental microbiology
Volume74
Issue number14
DOIs
StatePublished - Jul 1 2008

Fingerprint

Escherichia coli O157
Virulence Factors
virulence
Bacteriophages
genotype
Genotype
bacteriophages
gene
cattle
Sulfisoxazole
Saskatchewan
Genes
Ecotype
Colicins
Alberta
Quebec
ecotype
Ontario
Streptomycin
Tetracycline

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Genotypic characterization and prevalence of virulence factors among Canadian Escherichia coli O157:H7 strains. / Ziebell, Kim; Steele, Marina; Zhang, Yongxiang; Benson, Andrew; Taboada, Eduardo N.; Laing, Chad; McEwen, Scott; Ciebin, Bruce; Johnson, Roger; Gannon, Victor.

In: Applied and environmental microbiology, Vol. 74, No. 14, 01.07.2008, p. 4314-4323.

Research output: Contribution to journalArticle

Ziebell, K, Steele, M, Zhang, Y, Benson, A, Taboada, EN, Laing, C, McEwen, S, Ciebin, B, Johnson, R & Gannon, V 2008, 'Genotypic characterization and prevalence of virulence factors among Canadian Escherichia coli O157:H7 strains', Applied and environmental microbiology, vol. 74, no. 14, pp. 4314-4323. https://doi.org/10.1128/AEM.02821-07
Ziebell, Kim ; Steele, Marina ; Zhang, Yongxiang ; Benson, Andrew ; Taboada, Eduardo N. ; Laing, Chad ; McEwen, Scott ; Ciebin, Bruce ; Johnson, Roger ; Gannon, Victor. / Genotypic characterization and prevalence of virulence factors among Canadian Escherichia coli O157:H7 strains. In: Applied and environmental microbiology. 2008 ; Vol. 74, No. 14. pp. 4314-4323.
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