Genomic regions of coxsackievirus B3 associated with cardiovirulence

Cheol Lee, Elizabeth Maull, Nora Chapman, Steve Tracy, Charles Gauntt

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The molecular basis for cardiovirulence in the coxsackievirus B3 (CVB3) genome was examined in a murine model of acute myocarditis. Infectious cDNAs representing a highly cardiovirulent coxsackievirus R3 (CVB3(m)) and a noncardio-virulent (CVB3(o)) virus were used to construct infectious chimeric cDNAs. Assays of the resulting recombinant viruses for cardiovirulence in adolescent male CD-1 mice showed that the 5' nontranslated region (5' NTR) of the CVB3(m) genome plays the major role in determining cardiovirulence and that the genomic region encoding the capsid proteins has a minor additive effect in increasing cardiovirulence. Nucleotide sequences in the 5' NTR of COB3(m) and CVB3(o) differ at 23 positions; 14 are located in four stem-loop motifs of the secondary structure and may influence the cardiovirulent phenotype by regulating RNA or protein synthesis. A comparison of predicted amino acid sequences of capsid proteins in CVB3(m) and COB3(o) identified two amino acids as potential candidate contributors to cardiovirulence, i.e., amino acids at positions A207 (Asn-Asp) in the puff structure of the E-F loop of VP2 and A566 (Gln-Glu) in the C terminal of VP3 at the external surface. The data from this study and published literature support the conclusion that cardiovirulence of a CVB3 can depend on several regions of the genome.

Original languageEnglish (US)
Pages (from-to)341-347
Number of pages7
JournalJournal of Medical Virology
Volume52
Issue number3
DOIs
StatePublished - Jul 1 1997

Fingerprint

Enterovirus
Capsid Proteins
Genome
Complementary DNA
Viruses
Amino Acids
Myocarditis
Amino Acid Sequence
RNA
Phenotype

Keywords

  • Chimeric viruses
  • Coxsackieviruses group B
  • Mice
  • Myocarditis
  • Recombinant viruses

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Genomic regions of coxsackievirus B3 associated with cardiovirulence. / Lee, Cheol; Maull, Elizabeth; Chapman, Nora; Tracy, Steve; Gauntt, Charles.

In: Journal of Medical Virology, Vol. 52, No. 3, 01.07.1997, p. 341-347.

Research output: Contribution to journalArticle

Lee, Cheol ; Maull, Elizabeth ; Chapman, Nora ; Tracy, Steve ; Gauntt, Charles. / Genomic regions of coxsackievirus B3 associated with cardiovirulence. In: Journal of Medical Virology. 1997 ; Vol. 52, No. 3. pp. 341-347.
@article{36850b74cf464bbaa57c47492fcf7e91,
title = "Genomic regions of coxsackievirus B3 associated with cardiovirulence",
abstract = "The molecular basis for cardiovirulence in the coxsackievirus B3 (CVB3) genome was examined in a murine model of acute myocarditis. Infectious cDNAs representing a highly cardiovirulent coxsackievirus R3 (CVB3(m)) and a noncardio-virulent (CVB3(o)) virus were used to construct infectious chimeric cDNAs. Assays of the resulting recombinant viruses for cardiovirulence in adolescent male CD-1 mice showed that the 5' nontranslated region (5' NTR) of the CVB3(m) genome plays the major role in determining cardiovirulence and that the genomic region encoding the capsid proteins has a minor additive effect in increasing cardiovirulence. Nucleotide sequences in the 5' NTR of COB3(m) and CVB3(o) differ at 23 positions; 14 are located in four stem-loop motifs of the secondary structure and may influence the cardiovirulent phenotype by regulating RNA or protein synthesis. A comparison of predicted amino acid sequences of capsid proteins in CVB3(m) and COB3(o) identified two amino acids as potential candidate contributors to cardiovirulence, i.e., amino acids at positions A207 (Asn-Asp) in the puff structure of the E-F loop of VP2 and A566 (Gln-Glu) in the C terminal of VP3 at the external surface. The data from this study and published literature support the conclusion that cardiovirulence of a CVB3 can depend on several regions of the genome.",
keywords = "Chimeric viruses, Coxsackieviruses group B, Mice, Myocarditis, Recombinant viruses",
author = "Cheol Lee and Elizabeth Maull and Nora Chapman and Steve Tracy and Charles Gauntt",
year = "1997",
month = "7",
day = "1",
doi = "10.1002/(SICI)1096-9071(199707)52:3<341::AID-JMV18>3.0.CO;2-L",
language = "English (US)",
volume = "52",
pages = "341--347",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Genomic regions of coxsackievirus B3 associated with cardiovirulence

AU - Lee, Cheol

AU - Maull, Elizabeth

AU - Chapman, Nora

AU - Tracy, Steve

AU - Gauntt, Charles

PY - 1997/7/1

Y1 - 1997/7/1

N2 - The molecular basis for cardiovirulence in the coxsackievirus B3 (CVB3) genome was examined in a murine model of acute myocarditis. Infectious cDNAs representing a highly cardiovirulent coxsackievirus R3 (CVB3(m)) and a noncardio-virulent (CVB3(o)) virus were used to construct infectious chimeric cDNAs. Assays of the resulting recombinant viruses for cardiovirulence in adolescent male CD-1 mice showed that the 5' nontranslated region (5' NTR) of the CVB3(m) genome plays the major role in determining cardiovirulence and that the genomic region encoding the capsid proteins has a minor additive effect in increasing cardiovirulence. Nucleotide sequences in the 5' NTR of COB3(m) and CVB3(o) differ at 23 positions; 14 are located in four stem-loop motifs of the secondary structure and may influence the cardiovirulent phenotype by regulating RNA or protein synthesis. A comparison of predicted amino acid sequences of capsid proteins in CVB3(m) and COB3(o) identified two amino acids as potential candidate contributors to cardiovirulence, i.e., amino acids at positions A207 (Asn-Asp) in the puff structure of the E-F loop of VP2 and A566 (Gln-Glu) in the C terminal of VP3 at the external surface. The data from this study and published literature support the conclusion that cardiovirulence of a CVB3 can depend on several regions of the genome.

AB - The molecular basis for cardiovirulence in the coxsackievirus B3 (CVB3) genome was examined in a murine model of acute myocarditis. Infectious cDNAs representing a highly cardiovirulent coxsackievirus R3 (CVB3(m)) and a noncardio-virulent (CVB3(o)) virus were used to construct infectious chimeric cDNAs. Assays of the resulting recombinant viruses for cardiovirulence in adolescent male CD-1 mice showed that the 5' nontranslated region (5' NTR) of the CVB3(m) genome plays the major role in determining cardiovirulence and that the genomic region encoding the capsid proteins has a minor additive effect in increasing cardiovirulence. Nucleotide sequences in the 5' NTR of COB3(m) and CVB3(o) differ at 23 positions; 14 are located in four stem-loop motifs of the secondary structure and may influence the cardiovirulent phenotype by regulating RNA or protein synthesis. A comparison of predicted amino acid sequences of capsid proteins in CVB3(m) and COB3(o) identified two amino acids as potential candidate contributors to cardiovirulence, i.e., amino acids at positions A207 (Asn-Asp) in the puff structure of the E-F loop of VP2 and A566 (Gln-Glu) in the C terminal of VP3 at the external surface. The data from this study and published literature support the conclusion that cardiovirulence of a CVB3 can depend on several regions of the genome.

KW - Chimeric viruses

KW - Coxsackieviruses group B

KW - Mice

KW - Myocarditis

KW - Recombinant viruses

UR - http://www.scopus.com/inward/record.url?scp=0030741434&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030741434&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9071(199707)52:3<341::AID-JMV18>3.0.CO;2-L

DO - 10.1002/(SICI)1096-9071(199707)52:3<341::AID-JMV18>3.0.CO;2-L

M3 - Article

C2 - 9210047

AN - SCOPUS:0030741434

VL - 52

SP - 341

EP - 347

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 3

ER -