Genomic lesions associated with a different clinical outcome in diffuse large B-cell lymphoma treated with R-CHOP-21

Marta Scandurra, Michael Mian, Timothy Charles Greiner, Paola M V Rancoita, Cassio P. De Campos, Wing C. Chan, Julie Marie Vose, Ekaterina Chigrinova, Giorgio Inghirami, Annalisa Chiappella, Luca Baldini, Maurilio Ponzoni, Andres J M Ferreri, Silvia Franceschetti, Gianluca Gaidano, Santiago Montes-Moreno, Miguel A. Piris, Fabio Facchetti, Alessandra Tucci, Josep Fr NomdedeuThierry Lazure, Olivier Lambotte, Silvia Uccella, Graziella Pinotti, Giancarlo Pruneri, Giovanni Martinelli, Ken H. Young, Maria Grazia Tibiletti, Andrea Rinaldi, Emanuele Zucca, Ivo Kwee, Francesco Bertoni

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Despite recent therapeutic improvements, the clinical course of diffuse large B-cell lymphoma (DLBCL) still differs considerably among patients. We conducted this retrospective multi-centre study to evaluate the impact of genomic aberrations detected using a high-density genome wide-single nucleotide polymorphism-based array on clinical outcome in a population of DLBCL patients treated with R-CHOP-21 (rituximab, cyclophosphamide, doxorubicine, vincristine and prednisone repeated every 21 d). 166 DNA samples were analysed using the GeneChip Human Mapping 250K NspI. Genomic anomalies were analysed regarding their impact on the clinical course of 124 patients treated with R-CHOP-21. Unsupervised clustering was performed to identify genetically related subgroups of patients with different clinical outcomes. Twenty recurrent genetic lesions showed an impact on the clinical course. Loss of genomic material at 8p23.1 showed the strongest statistical significance and was associated with additional aberrations, such as 17p- and 15q-. Unsupervised clustering identified five DLBCL clusters with distinct genetic profiles, clinical characteristics and outcomes. Genetic features and clusters, associated with a different outcome in patients treated with R-CHOP, have been identified by arrayCGH.

Original languageEnglish (US)
Pages (from-to)221-231
Number of pages11
JournalBritish Journal of Haematology
Volume151
Issue number3
DOIs
StatePublished - Nov 1 2010

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Lymphoma, Large B-Cell, Diffuse
Cluster Analysis
Vincristine
Prednisone
Cyclophosphamide
Single Nucleotide Polymorphism
Genome
DNA
Population

Keywords

  • Comparative genomic hybridization
  • Hepatitis C virus
  • Lymphoma
  • Microarray
  • Prognosis

ASJC Scopus subject areas

  • Hematology

Cite this

Genomic lesions associated with a different clinical outcome in diffuse large B-cell lymphoma treated with R-CHOP-21. / Scandurra, Marta; Mian, Michael; Greiner, Timothy Charles; Rancoita, Paola M V; De Campos, Cassio P.; Chan, Wing C.; Vose, Julie Marie; Chigrinova, Ekaterina; Inghirami, Giorgio; Chiappella, Annalisa; Baldini, Luca; Ponzoni, Maurilio; Ferreri, Andres J M; Franceschetti, Silvia; Gaidano, Gianluca; Montes-Moreno, Santiago; Piris, Miguel A.; Facchetti, Fabio; Tucci, Alessandra; Nomdedeu, Josep Fr; Lazure, Thierry; Lambotte, Olivier; Uccella, Silvia; Pinotti, Graziella; Pruneri, Giancarlo; Martinelli, Giovanni; Young, Ken H.; Tibiletti, Maria Grazia; Rinaldi, Andrea; Zucca, Emanuele; Kwee, Ivo; Bertoni, Francesco.

In: British Journal of Haematology, Vol. 151, No. 3, 01.11.2010, p. 221-231.

Research output: Contribution to journalArticle

Scandurra, M, Mian, M, Greiner, TC, Rancoita, PMV, De Campos, CP, Chan, WC, Vose, JM, Chigrinova, E, Inghirami, G, Chiappella, A, Baldini, L, Ponzoni, M, Ferreri, AJM, Franceschetti, S, Gaidano, G, Montes-Moreno, S, Piris, MA, Facchetti, F, Tucci, A, Nomdedeu, JF, Lazure, T, Lambotte, O, Uccella, S, Pinotti, G, Pruneri, G, Martinelli, G, Young, KH, Tibiletti, MG, Rinaldi, A, Zucca, E, Kwee, I & Bertoni, F 2010, 'Genomic lesions associated with a different clinical outcome in diffuse large B-cell lymphoma treated with R-CHOP-21', British Journal of Haematology, vol. 151, no. 3, pp. 221-231. https://doi.org/10.1111/j.1365-2141.2010.08326.x
Scandurra, Marta ; Mian, Michael ; Greiner, Timothy Charles ; Rancoita, Paola M V ; De Campos, Cassio P. ; Chan, Wing C. ; Vose, Julie Marie ; Chigrinova, Ekaterina ; Inghirami, Giorgio ; Chiappella, Annalisa ; Baldini, Luca ; Ponzoni, Maurilio ; Ferreri, Andres J M ; Franceschetti, Silvia ; Gaidano, Gianluca ; Montes-Moreno, Santiago ; Piris, Miguel A. ; Facchetti, Fabio ; Tucci, Alessandra ; Nomdedeu, Josep Fr ; Lazure, Thierry ; Lambotte, Olivier ; Uccella, Silvia ; Pinotti, Graziella ; Pruneri, Giancarlo ; Martinelli, Giovanni ; Young, Ken H. ; Tibiletti, Maria Grazia ; Rinaldi, Andrea ; Zucca, Emanuele ; Kwee, Ivo ; Bertoni, Francesco. / Genomic lesions associated with a different clinical outcome in diffuse large B-cell lymphoma treated with R-CHOP-21. In: British Journal of Haematology. 2010 ; Vol. 151, No. 3. pp. 221-231.
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AU - Rancoita, Paola M V

AU - De Campos, Cassio P.

AU - Chan, Wing C.

AU - Vose, Julie Marie

AU - Chigrinova, Ekaterina

AU - Inghirami, Giorgio

AU - Chiappella, Annalisa

AU - Baldini, Luca

AU - Ponzoni, Maurilio

AU - Ferreri, Andres J M

AU - Franceschetti, Silvia

AU - Gaidano, Gianluca

AU - Montes-Moreno, Santiago

AU - Piris, Miguel A.

AU - Facchetti, Fabio

AU - Tucci, Alessandra

AU - Nomdedeu, Josep Fr

AU - Lazure, Thierry

AU - Lambotte, Olivier

AU - Uccella, Silvia

AU - Pinotti, Graziella

AU - Pruneri, Giancarlo

AU - Martinelli, Giovanni

AU - Young, Ken H.

AU - Tibiletti, Maria Grazia

AU - Rinaldi, Andrea

AU - Zucca, Emanuele

AU - Kwee, Ivo

AU - Bertoni, Francesco

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KW - Hepatitis C virus

KW - Lymphoma

KW - Microarray

KW - Prognosis

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